CircRNA circHIPK3 serves as a prognostic marker to promote glioma progression by regulating miR-654/IGF2BP3 signaling

Jin, Peng; Huang, Yi; Zhu, Penglei; Zou, Yi; Shao, Tingting; Ouyang, Wei

doi:10.1016/j.bbrc.2018.07.081

Cited by 151 publications

(125 citation statements)

References 22 publications

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“…28 Chen et al 14 found that circHIPK3 might be a new marker for HCC and may regulate HCC through a circHIPK3/miR-124/AQP3 axis. Jin et al 27 reported that circHIPK3 was upregulated in glioma and that circHIPK3 could influence the malignant behaviors of glioma cells via the circHIPK3/ miR-654/IGF2BP3 axis. Li et al 24 demonstrated that overexpression of circHIPK3 can effectively inhibit aggressiveness and metastasis of bladder cancer cells by targeting the miR-558/HPSE network.…”

Section: Discussionmentioning

confidence: 99%

<p>circHIPK3 Promotes Cell Proliferation and Migration of Gastric Cancer by Sponging miR-107 and Regulating BDNF Expression</p>

Wei¹,

Xu²,

Wei³

et al. 2020

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Background: Circular RNAs (circRNAs) play important regulatory roles in cancer development. However, the mechanisms by which circRNAs regulate gene expression in gastric cancer (GC) remain unclear. Methods: Human GC samples and their matched normal adjacent tissues were obtained from 30 patients to assess the expression of circHIPK3 and its relationship with GC proliferation and migration. A series of in vitro and in vivo functional experiments were carried out to elucidate the role of circHIPK3 in GC proliferation and migration, and its underlying molecular mechanisms. Results: Using a circRNA microarray we found a circRNA termed circHIPK3 that performed a significant regulatory role in GC. circHIPK3 was further confirmed to be upregulated in all GC tissues and cells tested. Furthermore, circHIPK3 levels were associated with Tumor & Lymph Node & Metastasis(TNM) stage (P = 0.032). The area under the receiver operating characteristic curve (ROC) was 0.743 (95% confidence interval 0.615-0.872; P = 0.001). CCK-8, colony formation, Transwell and EdU assays were performed to evaluate the effects of circHIPK3 on cell proliferation and migration in GC. Moreover, circHIPK3 was identified as a sponge of miR-107, and as such it regulated brain-derived neurotrophic factor (BDNF), which plays a pivotal role in the development of GC. Conclusion: circHIPK3 represents a novel potential biomarker and therapeutic target of GC.

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Section: Discussionmentioning

confidence: 99%

<p>circHIPK3 Promotes Cell Proliferation and Migration of Gastric Cancer by Sponging miR-107 and Regulating BDNF Expression</p>

Wei¹,

Xu²,

Wei³

et al. 2020

OTT

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“…The testis-specific circRNA, sex-determining region Y (circ-Sry), dependent on intron pairing-driven circularization, contained 16 miR-138 binding sites and acted as miR-138 sponge in mouse testis [6,36]. Also, circRNA HIPK3 exhibited sponging multiple miRNAs, including tumor suppressor miR-124, miR-7, miR-4288, miR-654 [46,[49][50][51]. Previous studies indicated that circ_0034642 and circ_0076248 promoted glioma proliferation and invasion in the miR-1205/BATF3 axis and miR-181a/SIRT1 axis, respectively [52,53].…”

Section: Functions Of Circular Rnasmentioning

confidence: 99%

“…For example, circRNAHIPK3 could sponge multiple miRNAs, containing miR-654 and miR-124-3p [46]. Jin et al proved that miR-654 was identified as a target of circHIPK3 while miR-654 targeted IGF2BP3, circHIPK3 promoted glioma progression through the circHIPK3/ miR-654/IGF2BP3 network [50]. Another study demonstrated that overexpression of circ-HIPK3 promoted proliferative and invasive capacities of glioma cells by sponging miR-124-3p up-regulating STAT3 level [80].…”

Section: Circrnas Act As Microrna Sponges On the Proliferation And Inmentioning

confidence: 99%

“…To date, the association between circRNA expression in tissues or peptides/proteins encoding by circRNA and clinical parameters have been largely reported. Circ_ 0034642, circ_0074362, cir-ITCH, circHIPK3 and cir-cCPA4 linked to clinical severity and poor prognosis in patients with glioma [50,53,87,113,114]. Wang et al proposed that the reduction of circ_0001649 was linked to larger tumor size and advanced WHO grade, indicating circ_0001649 may be an independent prognostic marker after surgery.…”

Section: Circrnas Are Potential Novel Biomarkers For Diagnosis and Prmentioning

confidence: 99%

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Functions and clinical significance of circular RNAs in glioma

et al. 2020

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CircRNAs are a class of single-stranded RNA molecules with a covalently closed loop structure and have been characterized by high stability, abundance, conservation, and display tissue/developmental stage-specific expression, furthermore, based on the abundance in distinct body fluids or exosomes, circRNAs present novel biomarkers and targets for the diagnosis and prognosis of cancers. Recently, the regulatory mechanisms of biogenesis and molecular functions, including miRNAs and RBPs sponge, translation as well as transcriptional and splicing regulation, have been gradually uncovered, although various aspects remained to be elucidated in combination with deep-sequence and bioinformatics. Accumulating studies have indicated that circRNAs are more enriched in neuronal tissues partly due to the abundance of specific genes promoting circularization, suggesting dysregulation of circRNAs is closely related to diseases of the nervous system, including glioma. In this review, we elaborate on the biogenesis, functions, databases as well as novel advances especially involved in the molecular pathways, highlight its great value as diagnostic or therapeutic targets in glioma.

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“…Previous studies have reported that circRNAs could be considered as ceRNA molecules or miRNA sponges and serve a crucial role in certain diseases (such as pathological hypertrophy and glioma) by influencing gene expression (7,10,(21)(22)(23)(24). For instance, Wang et al (21) demonstrated that heart-related circRNAs (HRCRs) could affect miR-223 activity in cardiac hypertrophy by acting as an endogenous miR-223 sponge.…”

Section: Discussionmentioning

confidence: 99%

Comprehensive circular RNA profiling reveals the regulatory role of circ_100242/miR-145 pathway in bladder cancer

et al. 2020

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Bladder cancer is a common genitourinary carcinoma with high morbidity and mortality rates. Circular RNAs (circRNAs), which are a type of single-stranded RNAs, have been characterized as stable, conserved and tissue-specific molecules in mammalian cells. The present study explored the circRNA expression profile in four bladder cancer tissues and matched normal samples by using microarray analysis. Furthermore, bioinformatics analyses were performed to investigate the potential function of dysregulated circRNAs in bladder cancer. The results demonstrated that 89 circRNAs were downregulated and 210 circRNAs were upregulated in bladder cancer tissues. The results from RT-qPCR demonstrated that hsa_circ_100241, hsa_circ_100242 and hsa_circ_101303 were markedly upregulated whereas hsa_circ_104510 was significantly downregulated in bladder cancer tissues compared with normal tissue. Following circRNA/microRNA (miRNA) interaction network generation via Cytoscape, it was demonstrated that hsa_circ_100242 contained a miRNA response element for miR-145-5p, which is a tumor suppressor in bladder carcinoma. In addition, results from the Kyoto Encyclopedia of Genes and Genomes analysis showed that the MAPK signaling pathway was the most significant pathway of the differentially expressed circRNAs in bladder cancer tissues. In conclusion, the present study demonstrated that circRNAs were dysregulated in bladder cancer tissues compared with matched normal samples. Pathway analysis was also performed to predict the binding of miRNAs to the dysregulated circRNAs. The results revealed that hsa_circ_100242 may be involved in bladder cancer initiation and progression by sponging miR-145. These findings may provide further insights into the functional and therapeutic roles of circRNAs in bladder cancer.

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CircRNA circHIPK3 serves as a prognostic marker to promote glioma progression by regulating miR-654/IGF2BP3 signaling

Cited by 151 publications

References 22 publications

<p>circHIPK3 Promotes Cell Proliferation and Migration of Gastric Cancer by Sponging miR-107 and Regulating BDNF Expression</p>

<p>circHIPK3 Promotes Cell Proliferation and Migration of Gastric Cancer by Sponging miR-107 and Regulating BDNF Expression</p>

Functions and clinical significance of circular RNAs in glioma

Comprehensive circular RNA profiling reveals the regulatory role of circ_100242/miR-145 pathway in bladder cancer

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