CircRNA ZNF609 functions as a competitive endogenous RNA to regulate FOXP4 expression by sponging miR‐138‐5p in renal carcinoma

Abstract

Circular RNA (circRNA) play important roles in the pathological processes of many diseases. By analyzing the results of the GSE100186 chip, we found that the expression of circRNA ZNF609 (circ‐ZNF609) was significantly increased in renal cell carcinoma. Recently, there are studies showing that circ‐ZNF609 can regulate cell proliferation and invasion ability of various cells. In this study, we investigated whether circ‐ZNF609 may affect cell invasion and proliferation in renal carcinoma. Quantitative reverse transcription polymerase chain reaction was performed to detect the expression of circ‐ZNF609 in renal carcinoma cell lines and renal epithelial cells. The direct interaction between microRNA‐138‐5p (miR‐138‐5p) and forkhead box P4 (FOXP4) or circ‐ZNF609 was confirmed by luciferase reporter assay and RNA immunoprecipitation assay. We use Cell Counting Kit‐8, 5‐ethynyl‐2′‐deoxyuridine, and Matrigel assays to assess the effect of miR‐138‐5p or circ‐ZNF609 on cell proliferation or invasion ability. And we found that circ‐ZNF609 is significantly increased in renal carcinoma cell lines. In addition, the high expression of circ‐ZNF609 promotes cell proliferation and invasion ability. In short, our current study reveals the role of the circ‐ZNF609/miR‐138‐5p/FOXP4 regulatory network in renal carcinoma and provides a new perspective for the pathogenesis of renal carcinoma.

Retracted: Circular RNA circFNDC3B protects renal carcinoma by miR‐99a downregulation

“…Recent studies have demonstrated that circRNA played efficacy in RC by mediating other factors. A study reflected circZNF609 exerted promoting carcinoma efficacies by modulating miR‐138‐5p in RC (Xiong, Zhang, & Song, ), other research reflected circciRs‐126 can be a landmark in kidney damage (Kolling et al, ). Furthermore, circFNDC3B exerted anticarcinoma efficacies by modulating miR‐1178‐3p in bladder carcinoma (Liu et al, ).…”

Circular RNA hsa_circ_0061825 (circ‐TFF1) contributes to breast cancer progression through targeting miR‐326/TFF1 signalling

“…In a word, these results validated that suppression of circ-TFF1 hindered breast cancer cell growth in vivo. 26 However, the investigation of circRNAs in cancers was still inadequate.…”

The circular RNA NRIP1 plays oncogenic roles by targeting miR-505 through the activation of AMPK and PI3K/AKT/mTOR pathways in the renal carcinoma cell lines

“…For instance, circ-HIAT1 acted as a metastatic inhibitor to prevent androgen receptor (AR)-accelerated RCC cell migration and invasion by de-regulating miR-195-5p [7]. circ-ZNF609 was significantly overproduced in RCC cell lines, and thus promoted the cell proliferation and invasion through a novel network that was comprised by circ-ZNF609 and miR-138-5p [17]. What's important, Zhang et al demonstrated that circNRIP1 promoted tumor metastasis in vivo by inducing proliferation and migration as well as the activation of AKT/mTOR cascade in GC [8].…”