Circular RNA: A new star of noncoding RNAs

Qu, Shibin; Yang, Xisheng; Li, X; Wang, Jianlin; Gao, Yuan; Shang, Runze; Sun, Wei; Dou, Kefeng; Li, Haimin

doi:10.1016/j.canlet.2015.06.003

Cited by 1,496 publications

(1,170 citation statements)

References 69 publications

(164 reference statements)

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“…It has been proposed that circRNAs may act as competing endogenous RNAs to bind proteins (protein sponges) [30,31]. Then, we focused on the sequence of hsa_circ_0001649 to find its potential RNA-binding proteins (RBPs) binding sites (starBase v2.0) [32], and we found that hsa_circ_0001649 had 6 potential protein binding sites for U2 auxiliary factor 65 kDa subunit (U2AF65), 5 potential protein binding sites for Eukaryotic initiation factor 4A-III (EIF4A3), and 1 potential protein binding site for Regulator of nonsense transcripts 1 (UPF1).…”

Section: Discussionmentioning

confidence: 99%

Hsa_circ_0001649: A circular RNA and potential novel biomarker for hepatocellular carcinoma

Qin

Liu

Huo

et al. 2016

CBM

392

299

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Abstract. BACKGROUND: It has been shown that circular RNA (circRNA) is associated with human cancers, however, few studies have been reported in hepatocellular carcinoma (HCC). OBJECTIVE: To estimate clinical values of a circular RNA, Hsa_circ_0001649, in HCC. METHODS: Expression level of hsa_circ_0001649 was detected in HCC and paired adjacent liver tissues by real-time quantitative reverse transcription-polymerase chain reactions (qRT-PCRs). Differences in expression level of hsa_circ_0001649 were analyzed using the paired t-test. Tests were performed between clinical information and hsa_circ_0001649 expression level by analysis of variance (ANOVA) or welch t-test and a receiver operating characteristics (ROC) curve was established to estimate the value of hsa_circ_0001649 expression as a biomarker in HCC. RESULTS: hsa_circ_0001649 expression was significantly downregulated in HCC tissues (p = 0.0014) based on an analysis of 89 paired samples of HCC and adjacent liver tissues and the area under the ROC curve (AUC) was 0.63. Furthermore, hsa_circ_0001649 expression was correlated with tumor size (p = 0.045) and the occurrence of tumor embolus (p = 0.017) in HCC. CONCLUSIONS: We first found hsa_circ_0001649 was significantly downregulated in HCC. Our findings indicate hsa_circ_0001649 might serve as a novel potential biomarker for HCC and may function in tumorigenesis and metastasis of HCC.

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Section: Discussionmentioning

confidence: 99%

Hsa_circ_0001649: A circular RNA and potential novel biomarker for hepatocellular carcinoma

Qin

Liu

Huo

et al. 2016

CBM

392

299

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“…CircRNA-targeted miRNA-gene network was constructed using Targetscan and miRanda. CircRNAs contain many miRNA response elements (MREs) [33], we then selected the top 5 predicted miRNA targets of 3 randomly differentially upregulated circRNAs (chr5: 51521674-51524828, chr3: 3137166-3138170 +, chr4: 59505289-59510641-) in tumor liver samples, meanwhile, the top 10 target genes for each miRNA were collected. Finally, the integrated circRNA-targeted miRNA-gene network was determined ( Figure 6).…”

Section: Mirna Binding Sites Prediction and Circrnatargeted Mirna-genmentioning

confidence: 99%

WITHDRAWN: Identification of aberrantly expressed circRNAs in subgroup J avian leucosis virus induced tumor livers by RNA sequencing

Zhang¹,

Yan²,

Lei³

et al. 2018

Oncotarget

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ALV-J (subgroup J avian leucosis virus) is a kind of oncogenic exogenousretrovirus and diseases associated with ALV-J have caused severe reproduction problems in the poultry industry worldwide. However, the pathogenesis of ALV-Jinduced tumor formation is still unclear. In recent years, circRNAs are validated to be related to cancer, which prompts us to explore whether aberrant expression of circRNAs exists in ALV-J-infected tumor samples. In this study, a total of 2822 differentially expressed circRNAs were detected by RNA sequencing in the tumor livers of ALV-J-susceptible chickens (n = 3) and the normal liver samples of ALV-J-resistant chickens (n = 3), among which 2808 circRNAs in the tumor liver samples were detected as unregulated compared to those in the normal liver samples. As circRNAs are considered as miRNA sponge, we predicted top 5 miRNA targets for 24 selected upregulated circRNAs in the ALV-J-induced tumor livers. CircRNA-miRNA-mRNA regulatory axes were also constructed to provide clear interaction relationship among them. Furthermore, Gene Ontology and KEGG pathway analyses were performed for miRNA target genes, the result of which implied that metabolism plays a key role in circRNAs alterations in ALV-J-induced tumor livers. In summary, we identified that circRNAs are involved in ALV-J-induced tumor formation in chickens. However, the pathogenicity mechanism still needs to be further explored.

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“…They are widely expressed in mammalian cells and play crucial roles in the regulation of gene expression at the transcriptional or post-transcriptional levels [3, 4] . Compared with traditional linear RNA, circRNAs do not have the free 3′ or 5′ ends, but form covalently closed continuous loops [5, 6]. This confers resistance to RNase R treatment, allowing circRNAs to be selectively enriched during sample processing and making them more suitable biomarkers than other types of RNA [7-9].…”

Section: Introductionmentioning

confidence: 99%

Microarray Expression Profile of Circular RNAs in Peripheral Blood Mononuclear Cells from Rheumatoid Arthritis Patients

Ouyang

Jiang

et al. 2017

Cell Physiol Biochem

135

102

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Background/Aims: Circular RNAs (circRNAs) compose a large class of RNAs that can be used as biomarkers in clinical blood samples. This study aimed to determine the expression of circRNAs in peripheral blood mononuclear cells (PBMCs) from rheumatoid arthritis (RA) patients to identify novel biomarkers for RA screening. Methods: We started with a microarray screening of circRNA changes in PBMCs from 5 RA patients and 5 healthy controls. We then confirmed the selected circRNA changes in PBMCs from 30 RA patients and 25 age- and sex-matched controls using the real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Spearman correlation test was performed to assess the correlation of circRNAs and clinical variables. Receiver operating characteristic (ROC) curve was calculated to evaluate the diagnostic value. Results: We identified and verified five circRNAs (092516, 003524, 103047, 104871, 101873) that were significantly elevated in PBMCs from RA patients. Among these RA patients, we detected no significant correlation between the five circRNAs and the disease severity, including disease activity score (DAS28), erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), and health assessment questionnaire (HAQ). Yet, ROC curve analysis suggested that circRNA_104871 has significant value of RA diagnosis (AUC=0.833, P<0.001), followed by circRNA_003524 (AUC = 0.683, P = 0.020), circRNA_101873 (AUC = 0.676, P = 0.026), and circRNA_103047 (AUC = 0.671, P = 0.030). Conclusions: This study suggests that increased expression of circRNAs circRNA_104871, circRNA_003524, circRNA_101873 and circRNA_103047 in PBMC from RA patients may serve as potential biomarkers for RA patient diagnosis.

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Circular RNA: A new star of noncoding RNAs

Cited by 1,496 publications

References 69 publications

Hsa_circ_0001649: A circular RNA and potential novel biomarker for hepatocellular carcinoma

Hsa_circ_0001649: A circular RNA and potential novel biomarker for hepatocellular carcinoma

WITHDRAWN: Identification of aberrantly expressed circRNAs in subgroup J avian leucosis virus induced tumor livers by RNA sequencing

Microarray Expression Profile of Circular RNAs in Peripheral Blood Mononuclear Cells from Rheumatoid Arthritis Patients

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