Circular RNA ABCB10 contributes to laryngeal squamous cell carcinoma (LSCC) progression by modulating the miR-588/CXCR4 axis

Abstract

Laryngeal squamous cell carcinoma (LSCC) is a common head and neck cancer with a high metastasis and poor prognosis. Circular RNAs (circRNAs) are a type of non-coding RNAs (ncRNAs) with regulatory function and broadly participate in cancer development. However, the correlation of circular RNA ABCB10 (circABCB10) with LSCC remains unclear. Here, we were interested in the role of circABCB10 in the modulation of LSCC progression. Our data demonstrated that the depletion of circABCB10 significantly inhibited the proliferation and induced the apoptosis of LSCC cells. Meanwhile, circABCB10 knockdown was able to remarkably reduce the invasion and migration of LSCC cells. Mechanically, circABCB10 served as a sponge for microRNAs-588 (miR-588) and miR-588 could target and down-regulated chemokine receptor 4 (CXCR4) expression in LSCC cells. The overexpression of CXCR4 or miR-588 inhibitor could reverse circABCB10 depletion-attenuated malignant phenotypes of LSCC cells. Functionally, the depletion of circABCB10 alleviated the tumor growth of LSCC cells in the tumorigenicity analysis of nude mice. The CXCR4 expression was decreased while the miR-588 expression was enhanced by circABCB10 depletion in vivo . Thus, we concluded that circABCB10 was involved in the malignant progression of LSCC by regulating miR-588/CXCR4 axis. Our finding provides new insights into the mechanism of circRHOT1 contributing to the development of LSCC. CircABCB10 and miR-588 may be used as potential targets for the treatment of LSCC.

High hsa_circ_0081621 expression indicates a poor prognosis of laryngeal squamous cell carcinoma: A bioinformatics analysis and retrospective clinical study

“…circ_0067934 is an oncogene promoting LSCC and may be a viable prognostic biomarker and target for diagnosis and treatment ( 28 ). Zhao et al ( 29 ) reported that circ ATP binding cassette subfamily B member 10 promotes LSCC progression and may act as a possible target in LSCC therapy. In addition, circRNA_103862 promotes LSCC proliferation by targeting the miR-493-5p/golgi membrane protein 1 axis and might serve as a potential prognosis marker and therapy target for LSCC ( 30 ).…”

CircRNAs: Roles in regulating head and neck squamous cell carcinoma

“…CircRNAs have shown great potential as therapeutic molecular tools in many cancers such as HNSCC ( 110 , 166 , 167 ), breast cancer ( 168 ), hepatocellular carcinoma ( 169 ), papillary thyroid cancer ( 170 ) gastric cancer ( 171 ). Compared with existing linear miRNA antagonists, circRNAs are structurally more stable, more resistant to degradation by nucleases without chemical modification, and well tolerated in vivo ( 172 ).…”

CircBFAR correlates with poor prognosis and promotes laryngeal squamous cell cancer progression through miR‐31‐5p/COL5A1 axis

“…A variety of circRNAs have been uncovered to be involved in the development of LSCC, 26 , 27 , 28 and we studied the function of circBFAR in LSCC. Guo et al 29 found that circBFAR was upregulated as an oncogene in patients with pancreatic ductal adenocarcinoma (PDAC), which was pertinent to tumor node metastasis and poor prognosis of PDAC patients.…”