Circular RNA-Centered Regulatory Networks in the Physiopathology of Cardiovascular Diseases

Gabriel, André F; Costa, Marina C.; Enguita, Francisco J.

doi:10.3390/ijms21020456

Cited by 11 publications

(8 citation statements)

References 79 publications

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“…In this sense, hsa_circ_0071542 was upregulated in children with fulminant myocarditis in leukocytes isolated from peripheral blood [24]. Nevertheless, this area remains mostly unexplored in DCM [22,25]. Recent studies have described several circRNA expression profiles in the DCM population compared to healthy patients.…”

Section: Discussionmentioning

confidence: 99%

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Circulating circRNA as biomarkers for dilated cardiomyopathy etiology

et al. 2021

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Dilated cardiomyopathy (DCM) is the third most common cause of heart failure. The multidisciplinary nature of testing — involving genetics, imaging, or cardiovascular techniques — makes its diagnosis challenging. Novel and reliable biomarkers are needed for early identification and tailored personalized management. Peripheral circular RNAs (circRNAs), a leading research topic, remain mostly unexplored in DCM. We aimed to assess whether peripheral circRNAs are expressed differentially among etiology-based DCM. The study was based on a case–control multicentric study. We enrolled 130 subjects: healthy controls (n = 20), idiopathic DCM (n = 30), ischemic DCM (n = 20), and familial DCM patients which included pathogen variants of (i) LMNA gene (n = 30) and (ii) BCL2-associated athanogene 3 (BAG3) gene (n = 30). Differentially expressed circRNAs were analyzed in plasma samples by quantitative RT-PCR and correlated to relevant systolic and diastolic parameters. The pathophysiological implications were explored through bioinformatics tools. Four circRNAs were overexpressed compared to controls: hsa_circ_0003258, hsa_circ_0051238, and hsa_circ_0051239 in LMNA-related DCM and hsa_circ_0089762 in the ischemic DCM cohort. The obtained areas under the curve confirm the discriminative capacity of circRNAs. The circRNAs correlated with some diastolic and systolic echocardiographic parameters with notable diagnostic potential in DCM. Circulating circRNAs may be helpful for the etiology-based diagnosis of DCM as a non-invasive biomarker. Key messages The limitations of cardiac diagnostic imaging and the absence of a robust biomarker reveal the need for a diagnostic tool for dilated cardiomyopathy (DCM). The circular RNA (circRNA) expression pattern is paramount for categorizing the DCM etiologies. Our peripheral circRNAs fingerprint discriminates between various among etiology-based DCM and correlates with some echocardiographic parameters. We provide a potential non-invasive biomarker for the etiology-based diagnosis of LMNA-related DCM and ischemic DCM.

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Section: Discussionmentioning

confidence: 99%

“…Regarding biological implications, circRNAs spring from introns or exons of their parental genes by backspliced circularization [25]. Hence, the ratio between linear and circular fractions affects gene expression.…”

Section: Discussionmentioning

confidence: 99%

Circulating circRNA as biomarkers for dilated cardiomyopathy etiology

et al. 2021

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show abstract

“…In this sense, hsa_circ_0071542 was upregulated in children with fulminant myocarditis in leukocytes isolated from peripheral blood [24]. Nevertheless, this area remains mostly unexplored in DCM [22,25]. Hence, further analysis of circRNAs among DCM etiologies might provide early, precise characterization of the disease and lead to novel pathological information, beyond the traditional biomarkers.…”

Section: Discussionmentioning

confidence: 99%

“…Regarding biological implications, circRNAs spring from introns or exons of their parental genes by back-spliced circularization [25]. Hence, the ratio between linear and circular fractions affects gene expression.…”

Section: Discussionmentioning

confidence: 99%

Circulating circRNA as Biomarkers For Dilated Cardiomyopathy Etiology

Costa

Calderón‐Domínguez

Mangas

et al. 2021

Preprint

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Background: Dilated cardiomyopathy (DCM) is the third most common cause of heart failure. The multidisciplinary nature of testing, -involving genetics, imaging, or cardiovascular techniques-, makes its diagnosis challenging. Novel and reliable biomarkers are needed for early identification and tailored personalized management. Peripheral circular RNAs (circRNAs), a leading research topic, remain mostly unexplored in DCM. We aimed to assess whether peripheral circRNAs are expressed differentially among etiology-based DCM. The study was based on a case-control multicentric study. Methods: We enrolled 130 subjects: healthy controls (n=20), idiopathic DCM (n=30), ischemic DCM (n=20) and familial DCM patients which included pathogen variants of i) LMNA gene (n=30) and ii) BCL2-associated athanogene 3 (BAG3) gene (n=30). Differentially expressed circRNAs were analyzed in plasma samples by quantitative RT-PCR and correlated to relevant systolic and diastolic parameters. The pathophysiological implications were explored through bioinformatics tools. Results: Four circRNAs were overexpressed compared to controls: hsa_circ_0003258, hsa_circ_0051238, and hsa_circ_0051239 in LMNA-related DCM; and hsa_circ_0089762 in the ischemic DCM cohort. The obtained areas under the curve confirm the discriminative capacity of circRNAs. Conclusions: The circRNAs correlated with some diastolic and systolic echocardiographic parameters with notable diagnostic potential in DCM. Circulating circRNAs may be helpful for the etiology-based diagnosis of DCM as a non-invasive biomarker.

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“…A substantial number of circRNAs have been identified ( Table 1 ) to be expressed differently for a variety of diseases, including CHD, atherosclerosis, myocardial fibrosis (MF), MI, 62 HF, hypertension, and aneurysm, among others. 63 , 64 , 65 circRNAs were also vertically studied, and their expression was reported as tissue- and stage-specific, indicating that circRNAs are also involved in cardiac cell differentiation. 66 Therefore, circRNAs have strong potential to be a biomarker of CVDs and signals that direct cell differentiation, and they may have important roles in future regenerative medicine.…”

Section: Main Textmentioning

confidence: 99%

Rapid Development of Targeting circRNAs in Cardiovascular Diseases

Zhang

Huo

et al. 2020

Molecular Therapy - Nucleic Acids

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Circular RNAs (circRNAs) are circularized, single-stranded RNAs that are covalently linked. With their abundance in tissues and developmental stage-specific expression, circRNAs participate in a variety of physiological and pathological processes. In this review, we discuss the development of circRNAs used as biomarkers and therapeutic targets for cardiovascular diseases (CVDs), focusing on recent discoveries and applications of exosomal circRNAs that highlight opportunities and challenges. Some studies have identified a spectrum of circRNAs that are differentially expressed in CVDs, while other studies further manipulated specific circRNA expression and showed an ameliorated pathogenic state such as ischemic injury, hypertrophy, and cardiac fibrosis. Studies and applications of circRNAs are being rapidly developed. We expect to see clinical use of circRNAs as biomarkers and targets for disease treatment in the near future.

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Circular RNA-Centered Regulatory Networks in the Physiopathology of Cardiovascular Diseases

Cited by 11 publications

References 79 publications

Circulating circRNA as biomarkers for dilated cardiomyopathy etiology

Circulating circRNA as biomarkers for dilated cardiomyopathy etiology

Circulating circRNA as Biomarkers For Dilated Cardiomyopathy Etiology

Rapid Development of Targeting circRNAs in Cardiovascular Diseases

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