Circular RNA circ_IRAK3 contributes to tumor growth through upregulating KIF2A via adsorbing miR-603 in breast cancer

Wang, Fang; Li, Jingruo; Li, Lin; Chen, Zhuo; Wang, Nan; Zhu, Mingzhi; Mi, Hailong; Xiong, Youyi; Guo, Guangcheng; Gu, Yuanting

doi:10.1186/s12935-022-02497-y

Cited by 8 publications

(4 citation statements)

References 63 publications

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“…As part of homeostasis, proliferation, inflammation, and apoptosis are highly regulated cellular processes [41] . Previous studies have shown that CircRNA is involved in various biological processes, including cell proliferation, apoptosis, invasion, and migration [42][43][44][45] . In the present study, in vitro cell experiments were used to thoroughly investigate the role of hsa cir 0072697 in the pathogenesis of AS.…”

Section: Discussionmentioning

confidence: 99%

Identification and Functional Analysis of Circular RNAs in Ankylosing Spondylitis Patient Peripheral Blood Mononuclear Cells

Huang,

Liu,

WANG

et al. 2024

Preprint

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Objectives:To analyze the function and roles of circRNAs in the peripheral blood mononuclear cells (PBMCs) of ankylosing spondylitis (AS) patients. Methods: The expression of AS-related circRNAs were detected by high-throughput RNA-sequencing within the PBMCs of 5 AS cases and healthy controls. After profiling circRNA expression in these samples, differentially expressed circRNAs (DECs) were identified, and a qPCR-based validation approach was used to confirm the differential expression of six of these DECs in patient samples. Spearman’s correlation tests and ROC curve analyses were further used to assess the relationship between disease severity and the expression of DECs of interest in AS patients, after which a putative circRNA-microRNAs (miRNAs) interaction network was constructed leading to the detection of six validated DECs with competing endogenous RNA (ceRNA) functionality. Besides, cell experiments were also performed to investigate the potential mechanism of key circRNA in AS. Results: 10,441 circRNAs were identified in these 10 PBMC samples, with 131 total DECs including 89 and 42 that were up- and down-regulated, respectively. In qPCR validation assays, patterns of hsa_circ_0000702, hsa_circ_0006209, hsa_circ_0047920, hsa_circ_0001543, hsa_circ_0072697 and hsa_circ_0005076 were confirmed to align well with RNA-seq results. In addition, the expression levels of hsa_circ_0006209, hsa_circ_0047920, and hsa_circ_0072697 were detected to be positively correlated with disease severity. ROC curve analyses suggested that hsa_circ_0072697 may offer value as a diagnostic biomarker for AS. Cell experiments indicated that hsa_circ_0072697 could suppress the progression of AS by targeting NF-κB pathway Conclusions: The identification of six circRNAs with putative ceRNA functionality in AS patients highlights potential molecular mechanisms governing this debilitating disease. However, further research will be necessary to formally confirm the roles of these DECs and their target miRNAs in AS. Further, hsa_circ_0072697 has a good diagnostic value in AS patients, and it could suppress the progression of AS by targeting NF-κB pathwa

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Section: Discussionmentioning

confidence: 99%

Identification and Functional Analysis of Circular RNAs in Ankylosing Spondylitis Patient Peripheral Blood Mononuclear Cells

Huang,

Liu,

WANG

et al. 2024

Preprint

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“…There are many circRNAs that can regulate EMT and metastasis and further contribute to the carcinogenic process, and the circular RNA-associated ceRNA network involved in biological processes may be quite crucial in the occurrence and development of cancer. For example, circ_0047303 may be a potential key regulator to mediate the upregulation of key angiogenesis-related genes, including HIF-1, EIF4E2, and VEGFA in TNBC, via sponging the tumor-suppressive miRNAs [64], circ_0000518 promotes breast cancer progression through the miRNA-1225-3p/SRY-box transcription factor 4 pathway, and it is oncogenic in BC [65], circ_0089153 is an oncogene in breast cancer, which enhances proliferation and metastasis via sponging miR-2467-3p/E2F6 [66], circ_IRAK3 exerts a promoting effect on BC progression by modulating the miR-603/KIF2A axis [67], circRNA_000554 up-regulates ZFP36 by sponging miR-182 [68], circ_RPPH1 up-regulates ARHGAP1 via sponging miR-542-3p [69], circ_ZFR up-regulates FABP7 by sponging miR-223-3p [70]. Hsa-circ-0061825 (circ-TFF1) contributes to BC via targeting miR-326/TFF1 [71], and circ-NOT2 can prevent breast cancer invasion, migration and EMT by regulating twist family BHLH transcription factor via targeting miR-409-3p/Twist1 [72].…”

Section: Circrna-associated Cerna Network In Breast Cancermentioning

confidence: 99%

Critical Roles of Circular RNA in Tumor Metastasis via Acting as a Sponge of miRNA/isomiR

Guo

Jia

Luo

et al. 2022

IJMS

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Circular RNAs (circRNAs), a class of new endogenous non-coding RNAs (ncRNAs), are closely related to the carcinogenic process and play a critical role in tumor metastasis. CircRNAs can lay the foundation for tumor metastasis via promoting tumor angiogenesis, make tumor cells gain the ability of migration and invasion by regulating epithelial-mesenchymal transition (EMT), interact with immune cells, cytokines, chemokines, and other non-cellular components in the tumor microenvironment, damage the normal immune function or escape the immunosuppressive network, and further promote cell survival and metastasis. Herein, based on the characteristics and biological functions of circRNA, we elaborated on the effect of circRNA via circRNA-associated competing endogenous RNA (ceRNA) network by acting as miRNA/isomiR sponges on tumor angiogenesis, cancer cell migration and invasion, and interaction with the tumor microenvironment (TME), then explored the potential interactions across different RNAs, and finally discussed the potential clinical value and application as a promising biomarker. These results provide a theoretical basis for the further application of metastasis-related circRNAs in cancer treatment. In summary, we briefly summarize the diverse roles of a circRNA-associated ceRNA network in cancer metastasis and the potential clinical application, especially the interaction of circRNA and miRNA/isomiR, which may complicate the RNA regulatory network and which will contribute to a novel insight into circRNA in the future.

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“…Among them, the levels of exosome-specific miR-583 and miR-603 were strongly correlated with the severity of AP [ 97 ]. MiR-583 and miR-603 are considered to be tumor suppressors, and their related studies are mainly focused on tumors such as breast cancer [ 98 ], hepatocellular carcinoma [ 99 ], and colorectal cancer [ 100 ]. There are no mechanistic studies on the association of miR-603 with AP or SIRS.…”

Section: Exosome-specific Ncrnasmentioning

confidence: 99%

Fighting Fire with Fire: Exosomes and Acute Pancreatitis-Associated Acute Lung Injury

et al. 2022

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Acute pancreatitis (AP) is a prevalent clinical condition of the digestive system, with a growing frequency each year. Approximately 20% of patients suffer from severe acute pancreatitis (SAP) with local consequences and multi-organ failure, putting a significant strain on patients’ health insurance. According to reports, the lungs are particularly susceptible to SAP. Acute respiratory distress syndrome, a severe type of acute lung injury (ALI), is the primary cause of mortality among AP patients. Controlling the mortality associated with SAP requires an understanding of the etiology of AP-associated ALI, the discovery of biomarkers for the early detection of ALI, and the identification of potentially effective drug treatments. Exosomes are a class of extracellular vesicles with a diameter of 30–150 nm that are actively released into tissue fluids to mediate biological functions. Exosomes are laden with bioactive cargo, such as lipids, proteins, DNA, and RNA. During the initial stages of AP, acinar cell-derived exosomes suppress forkhead box protein O1 expression, resulting in M1 macrophage polarization. Similarly, macrophage-derived exosomes activate inflammatory pathways within endothelium or epithelial cells, promoting an inflammatory cascade response. On the other hand, a part of exosome cargo performs tissue repair and anti-inflammatory actions and inhibits the cytokine storm during AP. Other reviews have detailed the function of exosomes in the development of AP, chronic pancreatitis, and autoimmune pancreatitis. The discoveries involving exosomes at the intersection of AP and acute lung injury (ALI) are reviewed here. Furthermore, we discuss the therapeutic potential of exosomes in AP and associated ALI. With the continuous improvement of technological tools, the research on exosomes has gradually shifted from basic to clinical applications. Several exosome-specific non-coding RNAs and proteins can be used as novel molecular markers to assist in the diagnosis and prognosis of AP and associated ALI.

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Circular RNA circ_IRAK3 contributes to tumor growth through upregulating KIF2A via adsorbing miR-603 in breast cancer

Cited by 8 publications

References 63 publications

Identification and Functional Analysis of Circular RNAs in Ankylosing Spondylitis Patient Peripheral Blood Mononuclear Cells

Identification and Functional Analysis of Circular RNAs in Ankylosing Spondylitis Patient Peripheral Blood Mononuclear Cells

Critical Roles of Circular RNA in Tumor Metastasis via Acting as a Sponge of miRNA/isomiR

Fighting Fire with Fire: Exosomes and Acute Pancreatitis-Associated Acute Lung Injury

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