Circular RNA circCSPP1 promotes the occurrence and development of colon cancer by sponging miR-431 and regulating ROCK1 and ZEB1

Wang, Jin; Zhou, Lei; Bingxin, Chen; Yu, Zhuwen; Zhang, Jianglei; Zhang, Zhe; Hu, Chenrui; Bai, Yanjin; Ruan, Xiaokang; Wang, Shengjia; Ouyang, Jingping; Wu, Airong; Zhao, Xin

doi:10.1186/s12967-022-03240-x

Cited by 6 publications

(4 citation statements)

References 53 publications

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“…In the other way, circCSPP1 positively regulates Rho-associated coiled-coil containing protein kinase 1 (ROCK1) abundance through miR-431 inhibition. ROCK1 further promotes cyclin D1 and CDK4 expression, which, in turn, phosphorylate Rb to allow the G0/G1-S phase transition [ 116 ].…”

Section: Circrna and Cell Cycle Regulators In Cancermentioning

confidence: 99%

Circular RNAs in Cell Cycle Regulation of Cancers

Thamjamrassri,

Ariyachet

2024

IJMS

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Cancer has been one of the most problematic health issues globally. Typically, all cancers share a common characteristic or cancer hallmark, such as sustaining cell proliferation, evading growth suppressors, and enabling replicative immortality. Indeed, cell cycle regulation in cancer is often found to be dysregulated, leading to an increase in aggressiveness. These dysregulations are partly due to the aberrant cellular signaling pathway. In recent years, circular RNAs (circRNAs) have been widely studied and classified as one of the regulators in various cancers. Numerous studies have reported that circRNAs antagonize or promote cancer progression through the modulation of cell cycle regulators or their associated signaling pathways, directly or indirectly. Mostly, circRNAs are known to act as microRNA (miRNA) sponges. However, they also hold additional mechanisms for regulating cellular activity, including protein binding, RNA-binding protein (RBP) recruitment, and protein translation. This review will discuss the current knowledge of how circRNAs regulate cell cycle-related proteins through the abovementioned mechanisms in different cancers.

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Section: Circrna and Cell Cycle Regulators In Cancermentioning

confidence: 99%

Circular RNAs in Cell Cycle Regulation of Cancers

Thamjamrassri,

Ariyachet

2024

IJMS

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“…For example, lncRNA LINC01578 modulates the NF-kB/YY1 axis to strengthen metastasis in colon cancer (7). CircRNA circCSPP1 combines with miR-431 to regulate ROCK1 and ZEB1, then facilitates the tumorigenesis of colon cancer (8). HOXD13 enhances PTPRN2 expression to aggravate colon cancer progression (9).…”

Section: Introductionmentioning

confidence: 99%

E2F3 accelerates the stemness of colon cancer cells by activating the STAT3 pathway

Gao

et al. 2023

Front. Oncol.

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IntroductionColon cancer is one of the most prevalent malignancies and causes of cancer-related deaths worldwide. Thus, further research is required to explicate the latent molecular mechanisms and look for novel biomarkers. E2F3 has been confirmed to be an oncogene in a variety of cancers. However, the particular regulation of E2F3 in colon cancer needs further investigation.MethodsThe self-renewal ability was detected through a sphere formation assay. The tumorigenic ability was measured through nude mice in vivo assay. The protein expression of genes was examined through a Western blot. The expression of E2F3 in tumor tissues was detected through an IHC assay. The resistance to cisplatin was assessed through the CCK-8 assay. The cell migration and invasion abilities were measured after upregulating or suppressing E2F3 through the Transwell assay.ResultsResults uncovered that E2F3 was upregulated in spheroid cells. In addition, E2F3 facilitates stemness in colon cancer. Moreover, E2F3 facilitated colon cancer cell migration and invasion. Finally, it was revealed that E2F3 affected the STAT3 pathway to modulate stemness in colon cancer. E2F3 served as a promoter regulator in colon cancer, aggravating tumorigenesis and stemness in colon cancer progression through the STAT3 pathway.ConclusionE2F3 may be a useful biomarker for anticancer treatment in colon cancer.

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“…The following supporting information can be downloaded at: , Figure S1: Role of circ_hsa_0008234 in HCT-8 cell line; Figure S2: GSEA analysis of ETS1 based on TCGA database; Table S1 [ 12 , 15 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 ]: Some circRNAs studies in colon cancer.…”

mentioning

confidence: 99%

“…cell line; FigureS2: GSEA analysis of ETS1 based on TCGA database; TableS1[12,15,[35][36][37][38][39][40][41][42][43][44]: Some circRNAs studies in colon cancer.…”

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confidence: 99%

CircRNA RNA hsa_circ_0008234 Promotes Colon Cancer Progression by Regulating the miR-338-3p/ETS1 Axis and PI3K/AKT/mTOR Signaling

et al. 2023

Cancers

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Circular RNAs (circRNAs) have been shown to play a crucial role in cancer occurrence and progression. This present work investigated the link between hsa_circ_0008234 and colon cancer. Data retrieved from GSE172229 was used to compare the circRNA profiles of colon cancer and surrounding non-tumorous tissues. The amount of RNA and protein in the molecules was determined using quantitative real-time PCR (qRT-PCR) and Western blot analysis, respectively. The cell proliferation ability was assessed using CCK8, EdU, colon formation, and nude mice tumorigenesis tests. Cell invasion and migration abilities were evaluated using transwell wound healing and mice lung metastasis model. Hsa_circ_0008234 piqued our interest because bioinformatics and qRT-PCR analyses revealed that it is upregulated in colon cancer tissue. Cell phenotypic studies suggest that hsa_circ_0008234 may significantly increase colon cancer cell aggressiveness. Mice experiments revealed that inhibiting hsa_circ_0008234 significantly reduced tumor growth and metastasis. Moreover, the fluorescence in situ hybridization experiment demonstrated that hsa_circ_0008234 is primarily found in the cytoplasm, implying that it potentially functions via a competitive endogenous RNA pathway. These findings indicated that hsa_circ_0008234 may act as a “molecular sponge” for miR-338-3p, increasing the expression of miR-338-target 3p’s ETS1. In addition, the traditional oncogenic pathway PI3K/AKT/mTOR signaling was found to be the potential downstream pathway of the hsa_circ_0008234/miR-338-3p/ETS1 axis. In conclusion, hsa_circ_0008234 increases colon cancer proliferation, infiltration, and migration via the miR-338-3p/ETS1/PI3K/AKT axis; therefore, it could serve as a target and a focus for colon cancer therapy.

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Circular RNA circCSPP1 promotes the occurrence and development of colon cancer by sponging miR-431 and regulating ROCK1 and ZEB1

Cited by 6 publications

References 53 publications

Circular RNAs in Cell Cycle Regulation of Cancers

Circular RNAs in Cell Cycle Regulation of Cancers

E2F3 accelerates the stemness of colon cancer cells by activating the STAT3 pathway

CircRNA RNA hsa_circ_0008234 Promotes Colon Cancer Progression by Regulating the miR-338-3p/ETS1 Axis and PI3K/AKT/mTOR Signaling

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