Circular RNA CircTTBK2 Facilitates Non-Small-Cell Lung Cancer Malignancy through the miR-873-5p/TEAD1/DERL1 Axis

Abstract

Aim: The underlying mechanisms by which circular RNAs (circRNAs) regulate non-small-cell lung cancer (NSCLC) progression remain elusive. This study investigated the role of circRNA circTTBK2 in NSCLC tumorigenesis. Materials & methods: Quantitative reverse transcriptase polymerase chain reaction analysis of circTTBK2 in NSCLC tissues and cell lines was performed. Cell proliferation, migration, invasion and tumorigenesis were confirmed in vitro and in vivo using CCK-8, EdU incorporation, Transwell assays and xenograft technique. The circTTBK2/miR-873-5p/TEAD1/DERL1 axis was verified by RNA immunoprecipitation, chromatin immunoprecipitation and luciferase reporter assays. Results: Overexpressed circTTBK2 in NSCLC tissues indicates poor prognosis of NSCLC patients. circTTBK2 harbors miR-873-5p, and miR-873-5p directly targets TEAD1. TEAD1 transcriptionally activates DERL1. Conclusion: This study revealed a novel machinery of circTTBK2/miR-873-5p/TEAD1/DERL1 for NSCLC tumorigenesis.

Ferroptosis contributes to the progression of female-specific neoplasms, from breast cancer to gynecological malignancies in a manner regulated by non-coding RNAs: Mechanistic implications

“…4 ) ( Table 3 ). CircTTBK2 (tau tubulin kinase 2) is one of these tumor-associated circRNAs that is markedly expressed in gliomas and is responsible for regulating tumor cell proliferation, migration, and invasion [ 139 ]. This circRNA also controls cell metabolism by sponging MIR1283 , MIR520B , MIR217 , and MIR761 , among which MIR761 can potentially modulate the ferroptotic flux in HCC via targeting MFN2 [ [140] , [141] , [142] ].…”