Circular RNA expression alteration in whole blood of premature infants with periventricular white matter damage

Qiao, Lixing; Mo, Si-Si; Zhou, Yan; Zhang, Yi; Li, Bangbang; Wu, She–Ching; Lin, Lili; Zhu, Lihua; Zhao, Ran

doi:10.1016/j.ygeno.2020.03.027

Cited by 10 publications

(10 citation statements)

References 45 publications

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“…According to analysis in the databases [ 22 – 24 ], circFOXK2, also named hsa_circ_0000816, was located in 2–3 exons of the FOXK2 gene. Qiao et al reported that the upregulation of circFOXK2 is associated with periventricular white matter damage (PWMD) of premature infants [ 38 ]. Also, circFOXK2 may participate in the regulation of myotonic dystrophy [ 39 ].…”

Section: Discussionmentioning

confidence: 99%

CircRNA circFOXK2 facilitates oncogenesis in breast cancer via IGF2BP3/miR-370 axis

Zhang

Liu

Jiang

et al. 2021

Aging

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Metastasis is the leading cause of breast cancer (BC)-related deaths. Circular RNAs (circRNAs) have emerged as essential regulators for cancer progression and metastasis. Therefore, the objective of this study was to investigate the role of circRNAs in BC metastasis and related mechanism. In this study, we established the BC cell line with high or low potential of metastasis. RNA sequencing, migration and invasion assay, Fluorescence in situ hybridization, luciferase report assay, circRNA pulldown, and transmission electron microscopy were performed to elucidate the molecular mechanism. The results showed that circRNA circFOXK2 was significantly increased in BC cells with high metastatic ability, and the upregulation of circFOXK2 was correlated with poor clinicopathological characteristics. Functional experiments demonstrated that overexpression of circFOXK2 promoted migration and invasion of BC cells. Also. circFOXK2 could act with IGF2BP3, an RNA-binding protein, and miR-370 to synergistically promote BC metastasis. Moreover, miR-370 could be transferred through exosomes to enhance the metastatic ability of recipient cells. In conclusion, circFOXK2 functions as a key regulator in BC metastasis, and the role of circFOXK2 on BC metastasis is tightly associated with the involvement of IGF2BP3 and miR-370. CircFOXK2 might serve as a potential biomarker for the diagnosis and treatment of BC.

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Section: Discussionmentioning

confidence: 99%

CircRNA circFOXK2 facilitates oncogenesis in breast cancer via IGF2BP3/miR-370 axis

Zhang

Liu

Jiang

et al. 2021

Aging

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“…We have previously carried out a preliminary study on circRNA expression signatures in peripheral venous blood from PWMD patients and performed functional analysis (Qiao et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

“…We collected the samples following the protocol described in our previous study (Qiao et al, 2020). Thereafter, a total of six samples (N12, N15, N27 from PWMD group and N13, N55, N78 from control group) were used for RNA sequencing (Table 1).…”

Section: Samplesmentioning

confidence: 99%

Dissecting the Roles of LncRNAs in the Development of Periventricular White Matter Damage

et al. 2021

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Long non-coding RNA (LncRNA) has high expression in the brain. Animal studies have shown that lncRNA plays an important role in brain functions and mediates the development of many neurological diseases. However, data on the expression of lncRNAs and the clinical significance in prematurely born infants with diseases such as periventricular white matter damage (PWMD) remains scant. Here, we compared the expression of the lncRNAs in whole blood samples obtained from prematurely born infants with PWMD with samples from prematurely born infants without PWMD. Our data demonstrated differential expression of the lncRNAs between the two groups. Further, we showed that the lncRNAs play important roles in the development of PWMD. Our findings give insights into the functions of the lncRNAs in PWMD and provide evidence for the improvement of diagnostic and treatment strategies in infants with PWMD.

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“…The GSE131475 dataset was used to compare circRNA expression levels in peripheral blood of preterm infants with periventricular white matter damage (PWMD) and without damage. [ 16 ].…”

Section: Methodsmentioning

confidence: 99%

“…In addition to its targeted regulatory relationship with mRNA, miRNA can also form ceRNA, circRNA-miRNA-mRNA, or a competitive binding miRNA mechanism of circRNA-miRNA-lncRNA to mRNA and genes including circRNA and lncRNA, which is thought to play an important role in biological behaviour. Comparisons of peripheral blood circRNA sequencing data from patients with premature ventricular white matter injury revealed 119 differentially expressed circRNAs, with the same preliminary construction of a miRNA-circRNA network [ 16 ]. The above studies revealed differential genetic changes during brain injury among preterm infants, with the exact mechanisms being unclear.…”

Section: Introductionmentioning

confidence: 99%

A Potential ceRNA Network for Neurological Damage in Preterm Infants

Jin

Liang

Cai

2021

BioMed Research International

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Objective. This study is aimed at identifying key genes involved in neurological damage in preterm infants and at determining their potential circRNA-miRNA-mRNA regulatory mechanisms. Methods. Differentially expressed miRNAs, mRNAs, and circRNAs were downloaded from the GEO database. GO and KEGG enrichment analyses were used to determine possible relevant functions of differentially expressed mRNAs. The TTRUST database was used to predict differential TF-mRNA regulatory relationships. Then, CircMIR, miRDB, TargetScan and miRTarBase were then used to map circRNA/miRNA-TF/mRNA interaction networks. Finally, GSEA enrichment analysis was performed on the core transcription factors. Results. A total of 640 mRNAs, 139 circRNAs, and 206 differentially expressed miRNAs associated with neurological injury in preterm infants were obtained. Based on the findings of Cytoscape and PPI network analysis, the hsa_circ_0008439-hsa-mir-3665-STAT3-MMP3 regulatory axis was established. GSEA analysis revealed that suppressed expression levels of STAT3 were associated with upregulated oxidative phosphorylation pathways in the neurological injury group of preterm infants. Conclusions. The circRNA-miRNA-TF-mRNA regulatory network of neurological injury in preterm infants can be used to elucidate on the pathogenesis of brain injury and help us with the early detection of brain injury in preterm infants.

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Circular RNA expression alteration in whole blood of premature infants with periventricular white matter damage

Cited by 10 publications

References 45 publications

CircRNA circFOXK2 facilitates oncogenesis in breast cancer via IGF2BP3/miR-370 axis

CircRNA circFOXK2 facilitates oncogenesis in breast cancer via IGF2BP3/miR-370 axis

Dissecting the Roles of LncRNAs in the Development of Periventricular White Matter Damage

A Potential ceRNA Network for Neurological Damage in Preterm Infants

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