Circular RNA expression in pancreatic ductal adenocarcinoma

Zhang, Qian; Wang, Jin Yan; Zhou, Shu; Yang, Su Jin; Zhong, Shan

doi:10.3892/ol.2019.10624

Cited by 21 publications

(23 citation statements)

References 40 publications

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“…22,23 CircRNAs had been proved to be involved in the development, chemotherapy resistance, proliferation, invasion, and prognosis of PC. [24][25][26] In our study, the level of circHIPK3 was increased in PC tissues, GEMresistant PC tissues, and GEM-resistant PC cells. PC patients with high expression of circHIPK3 displayed poorer overall survival than those with lower circHIPK3 expression.…”

Section: Discussionsupporting

confidence: 53%

<p>CircHIPK3 Promotes Gemcitabine (GEM) Resistance in Pancreatic Cancer Cells by Sponging miR-330-5p and Targets RASSF1</p>

Liu¹,

Li²,

Luo³

et al. 2020

CMAR

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Background: Pancreatic cancer is one of the most common malignant diseases in the world. Gemcitabine chemotherapy remains the most important clinical treatment. However, research found that pancreatic cancer cells have chemoresistance to gemcitabine and the effect is not satisfactory. Therefore, it is urgent to find an effective early diagnosis and treatment strategy. Circular RNA is one of the most popular prognostic biomarkers in GEM-resistant PC. Materials and Methods: The present study was designed to evaluate the role of circHIPK3 in PC. The expression of circHIPK3 in PC tissues and cells and its effect on proliferation, migration, invasion, EMT, and apoptosis were investigated in vitro; its effect on tumor xenografts was assessed in vivo. Used bioinformation analysis to predict which miRNAs could potentially interact with circHIPK3, mRNA, and miR-330-5p. Results: RT-PCR showed that the level of circHIPK3 was increased in PC tumor tissues; moreover, circHIPK3 was also increased in GEM-resistant PC tumors tissues and GEMresistant PC cells. Sh-circHIPK3 could knockdown circHIPK3 in PANC-1-GEM and SW-1990-GEM and could significantly inhibit cell proliferation, invasion, migration, EMT and enhance cell apoptosis, compare with control group, the tumor xenografts of circHIPK3 knockdown group were significantly smaller. CircHIPK3 served as a sponge for miR-330-5p, and miR-330-5p directly bound to the 3′ UTR of RASSF1 were revealed by dual luciferase assay and RIP in PC cells. CircHIPK3 knockdown of RASSF1 expression could neutralize the cytological function of PC cells by miR-330-5p inhibitor mediated GEM-resistance. Conclusion: CircHIPK3 promotes gemcitabine (GEM) resistance in pancreatic cancer cells by targeting RASSF1 via miR-330-5p and regulates proliferation, invasive, migration, EMT, and apoptosis. Our research revealed that circHIPK3 may be a novel biomarker in GEMresistant PC and could be used as a prognostic target.

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Section: Discussionsupporting

confidence: 53%

<p>CircHIPK3 Promotes Gemcitabine (GEM) Resistance in Pancreatic Cancer Cells by Sponging miR-330-5p and Targets RASSF1</p>

Liu¹,

Li²,

Luo³

et al. 2020

CMAR

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“…CircRNAs are widely considered to act as vital regulators under multiple physiological and pathological conditions. Studies have shown that circRNAs are abundantly expressed in pancreatic tissues and are related to the development and progression of pancreatic diseases (Xu et al, 2015;Zhang et al, 2019). Stoll et al (2018) reported that circHIPK3 and ciRS-7/CDR1as impaired insulin secretion by regulating the expression of key β-cell genes in diabetic conditions.…”

Section: Discussionmentioning

confidence: 99%

CircRNA Expression Profiles and the Potential Role of CircZFP644 in Mice With Severe Acute Pancreatitis via Sponging miR-21-3p

et al. 2020

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Severe acute pancreatitis (SAP) is the most serious type of pancreatitis with high morbidity and mortality. The underlying mechanism behind SAP pathogenesis is complex and remains elusive. Circular RNAs (circRNAs) are emerging as vital regulators of gene expression in various diseases by sponging microRNAs (miRNAs). However, the roles of circRNAs in the pathophysiology of SAP remain unknown. In the present study, next-generation RNA sequencing was utilized to identify circRNA transcripts in the pancreatic tissues from three SAP mice and three matched normal tissues. The differentially expressed circRNAs were confirmed by real-time PCR, and the biological functions of their interaction with miRNAs and mRNAs were analyzed. Our results demonstrate that 56 circRNAs were differentially expressed in SAP mice compared with normal controls. Six differentially expressed circRNAs were confirmed with the sequencing data. Importantly, we characterized a significantly downregulated circRNA derived from the ZFP664 gene in SAP. CircZFP644 was found to be negatively correlated with miR-21-3p, with a perfectly matched binding sequence to miR-21-3p. In conclusion, CircZFP644 may play an important role in the pathogenesis of SAP through sponging miR-21-3p. Our findings may provide novel insights regarding the workings of the pathophysiological mechanism of SAP and offer novel targets for SAP.

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“…One study identified circRNAs in six pairs of PDAC and para-cancerous tissues using a microarray (74). Additional microarray data revealed that there were 115 upregulated and 141 downregulated circRNAs in PDACs (75). Another study uncovered that 453 circRNAs were differentially expressed and were significantly different in extracellular vesicles isolated from the plasma of 8 PDAC patients or healthy controls (76).…”

Section: Circrnas In Pdacmentioning

confidence: 99%