Circular RNA expression profile in peripheral whole blood of lung adenocarcinoma by high

Mu, Yinyu; Xie, Fuyi; Huang, Yunfei; Yang, Dongdong; Xu, Guodong; Wang, Chunnian; Wu, Qing

doi:10.1097/md.0000000000017601

Cited by 9 publications

(9 citation statements)

References 26 publications

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“…Previous evidence has recently indicated that circRNAs are closely implicated in the malignant tumor progression of NSCLC. [25][26][27] In terms of the PI3K/AKT pathway, several circRNAs were declared to modulate this signaling pathway in NSCLC. For example, the PI3K inhibitor-LY264002 could reverse the promoting effect of circGFRA1 overexpression on cell viability, colony formation, and expression of cyclin A1 and cyclin B1 in NSCLC cells in vitro and in vivo.…”

Section: Discussionmentioning

confidence: 99%

<p>Knockdown of circPRKCA Restrained Cell Growth, Migration, and Invasion of NSCLC Cells Both in vitro and in vivo via Regulating miR-330-5p/PDK1/AKT Pathway</p>

Bai¹,

Peng²,

Sun³

2020

CMAR

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Background: Protein kinase Cα (PRKCA) is an oncogene in multiple cancers including non-small-cell lung cancer (NSCLC) and can be transcribed into a number of circular PRKCAs (circPRKCAs). Here, we aimed to elaborate the role and mechanism of circPRKCA_024 (circPRKCA) in malignant progression of NSCLC. Methods: Expression of circPRKCA, miRNA (miR)-330-5p and 3-phosphoinositidedependent protein kinase-1 (PDK1) was measured by real-time quantitative PCR and Western blotting, and their relationship was testified by dual-luciferase reporter assay, RNA immunoprecipitation, and RNA pull-down assay. Cell behaviors were evaluated by cell counting kit (CCK)-8, flow cytometry, and transwell assays. AKT activity was confirmed by Western blotting. Xenograft experiment assessed tumor growth. Results: Expression of circPRKCA and PDK1 was upregulated, and miR-330-5p was downregulated in NSCLC tissues and cell lines. High circPRKCA was correlated with TNM stage and lymph node metastasis of NSCLC patients. Silencing circPRKCA could suppress cell viability, migration, and invasion in A549 and H1299 cells, accompanied with apoptosis rate promotion. Moreover, circPRKCA knockdown retarded tumor growth of A549 cells in vivo. Molecularly, miR-330-5p was sponged by circPRKCA, and PDK1 was a target of miR-330-5p. Inhibiting miR-330-5p could attenuate the suppression of circPRKCA knockdown on cell growth, migration, and invasion; contrarily, promoting miR-330-5p caused inhibition on those cell behaviors by downregulating PDK1. Analogously, AKT activity was suppressed by circPRKCA downregulation and miR-330-5p upregulation in NSCLC cells both in vitro and in vivo. Conclusion: Depleting circPRKCA inhibited PDK1 to suppress NSCLC cell malignant behaviors through miR-330-5p/PDK1/AKT pathway.

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Section: Discussionmentioning

confidence: 99%

<p>Knockdown of circPRKCA Restrained Cell Growth, Migration, and Invasion of NSCLC Cells Both in vitro and in vivo via Regulating miR-330-5p/PDK1/AKT Pathway</p>

Bai¹,

Peng²,

Sun³

2020

CMAR

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“…Previous evidence has revealed the involvement of circRNAs in human diseases [ 7 ]. Multiple expression profiles of circRNAs have also been uncovered in tissues [ 8 ], peripheral whole blood [ 9 ], and plasma exosomes [ 10 ] from LUAD patients. Several circRNAs are suggested to be noninvasive diagnostic biomarkers for LUAD [ 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

A Regulatory Axis of circ_0008193/miR-1180-3p/TRIM62 Suppresses Proliferation, Migration, Invasion, and Warburg Effect in Lung Adenocarcinoma Cells Under Hypoxia

et al. 2020

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Background Expression profiles of circular ribonucleic acids (circRNAs) have been recently reported in lung cancers including lung adenocarcinoma (LUAD). Hypoxia is a hallmark of lung cancers. However, the role of hsa_circ_0008193 (circ_0008193) in LUAD under hypoxia remains to be illuminated. Material/Methods Gene expression levels were detected using real-time quantitative polymerase chain reaction and western blotting. Cell proliferation, migration, invasion, and Warburg effect were detected using 3-(4, 5-dimethylthiazol-2-yl)-2, 5 diphenyltetrazolium bromide assay, transwell assays, special kits, and xenograft experiments. The relationship among circ_0008193, micro (mi)RNA (miR)-1180-3p, and tripartite motif containing 62 (TRIM62) was confirmed by dual-luciferase reporter assay and RNA immunoprecipitation. Results Expression of circ_0008193 was downregulated in human LUAD tumor tissues and cell lines (A549 and H1975), accompanied by miR-1180-3p upregulation and TRIM62 downregulation. Moreover, circ_0008193 downregulation was correlated with tumor size and lymph node metastasis. Functionally, circ_0008193 overexpression inhibited cell viability, glucose uptake, lactate production, migration, and invasion, as well as expression of hexokinase II, lactate dehydrogenase A, matrix metalloproteinase 2 (MMP2), and MMP9 in hypoxic LUAD cells in vitro . Furthermore, tumor growth of A549 cells in vivo was also hindered by circ_0008193 overexpression. Mechanically, circ_0008193 regulated TRIM62 expression via sponging miR-1180-3p, and TRIM62 was targeted by miR-1180-3p. Both miR-1180-3p upregulation and TRIM62 downregulation could abolish the suppressive role of circ_0008193 in LUAD cells. Conclusions Upregulating circ_0008193 inhibited LUAD cell proliferation, migration, invasion, and Warburg effect under hypoxia in vitro and in vivo through regulation of the miR-1180-3p/TRIM62 axis.

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“…In another study, 356 circRNAs were dysregulated in lung adenocarcinoma, including 204 upregulated circRNAs and 152 downregulated circRNAs ( 55 ). By utilizing circRNA chips, Mu et al ( 56 ) identified and annotated a total of 10,566 circRNAs in the peripheral whole blood of patients with lung adenocarcinoma. Amongst these, 78.14% of the circRNAs were exonic, and 3,009 circRNAs were upregulated, whereas 1,381 circRNAs were downregulated.…”

Section: Circrnas and Nsclcmentioning

confidence: 99%

Emerging roles of circular RNAs in non‑small cell lung cancer (Review)

Liu

Qiu

et al. 2021

Oncol Rep

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Circular RNAs (circRNAs) are a class of novel endogenous transcripts with limited protein-coding abilities. CircRNAs have been demonstrated to function as critical regulators of tumor development and distant metastasis through binding to microRNAs (miRNAs) and interacting with RNA-binding proteins, thereby regulating transcription and translation. Emerging evidence has illustrated that certain circRNAs can serve as biomarkers for diagnosis and prognosis of cancer, and/or serve as potential therapeutic targets. Expression of functional circRNAs is commonly dysregulated in cancer and this is correlated with advanced Tumor-Node-Metastasis stage, lymph node status, distant metastasis, poor differentiation and shorter overall survival of cancer patients. Recently, an increasing number of studies have shown that circRNAs are closely associated with NSCLC. Functional experiments have revealed that circRNAs are intricately associated with the pathological progression of NSCLC. The present review provides an overview of the regulatory effect of circRNAs in the development and progression of NSCLC, taking into consideration various physiological and pathological processes, such as proliferation, apoptosis, invasion and migration, and their potential value as biomarkers and therapeutic targets.

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Circular RNA expression profile in peripheral whole blood of lung adenocarcinoma by high

Cited by 9 publications

References 26 publications

<p>Knockdown of circPRKCA Restrained Cell Growth, Migration, and Invasion of NSCLC Cells Both in vitro and in vivo via Regulating miR-330-5p/PDK1/AKT Pathway</p>

<p>Knockdown of circPRKCA Restrained Cell Growth, Migration, and Invasion of NSCLC Cells Both in vitro and in vivo via Regulating miR-330-5p/PDK1/AKT Pathway</p>

A Regulatory Axis of circ_0008193/miR-1180-3p/TRIM62 Suppresses Proliferation, Migration, Invasion, and Warburg Effect in Lung Adenocarcinoma Cells Under Hypoxia

Emerging roles of circular RNAs in non‑small cell lung cancer (Review)

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