Circular RNA expression profiles significantly altered in UVA‑irradiated human dermal fibroblasts

Lin, Mengbi; Zheng, Yue; Li, Qian; Liu, Yufang; Xu, Qingfang; Li, Yuying; Wei, Lai

doi:10.3892/etm.2020.9292

Cited by 5 publications

(10 citation statements)

References 34 publications

(40 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We recently found that there were 128 differentially expressed circRNA in HDFs chronic light injury model through circRNA chip technology. Moreover, it was verified that circ_0011129 has anti-photoaging effect in HDF cells [ 25 ]. However, the instability in vivo and low delivery efficiency to the target cell limited their widely application.…”

Section: Discussionmentioning

confidence: 99%

“…Previously, we found that circ_0011129 acts as a miR-6732-5p adsorption sponge to inhibit the reduction of type I collagen and the denaturation and accumulation of elastin in HDF exposed to UVA at the same time [ 25 ]. Consistent with previous studies, circ_0011129, delivered into HDF cells using 3D-sEVs, interfered with the process of cell photoaging by decreasing SA-β-gal activity, preventing the expression of MMP1, MMP3, and Cathepsin K, and accelerating the expression of collagen I, collagen III, and elastin.…”

Section: Discussionmentioning

confidence: 99%

“…To sum up, the best way to intervene skin photoaging is to regulate collagen and elastin at the same time. We have identified recently that circ_0011129 acts as a miR-6732-5p adsorption sponge to inhibit photoaging in vitro [ 25 ]. These suggested that circRNA has an excellent application prospect.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Circ_0011129 Encapsulated by the Small Extracellular Vesicles Derived from Human Stem Cells Ameliorate Skin Photoaging

Zhang

Yao

et al. 2022

IJMS

Self Cite

View full text Add to dashboard Cite

Photoaging is not only the main cause of skin aging caused by exogenous factors, it is also related to a variety of skin diseases and even malignant tumors. Excessive and repeated exposure to ultraviolet radiation, especially UVA induces oxidative stress, DNA damage, inflammation, and collagen and elastin degeneration, ultimately leads to skin photoaging, manifested by skin redness, coarse wrinkles, and pigmentation even skin cancer. There has been a large demand of effective prevention and medications but approaches in the current management of photoaging are very limited. In the previous study, we found that a non-coding circular RNA circ_0011129 acts as a miR-6732-5p adsorption sponge to inhibit the reduction of type I collagen and the denaturation and accumulation of elastin in UVA-induced HDF cells photoaging model. However, in vivo instability and efficient delivery to the target cell of circRNA is a major challenge for its clinical application. Therefore, improving its stability and delivery efficiency are desired. In this study, we proposed a strategy of delivering circ_0011129 with small extracellular vesicles (sEVs) from human adipose-derived stem cells (hADSCs) to intervene in the photoaging process. The results showed that sEVs from hADSCs in 3D bioreactor culture (3D-sEVs) can prevent photoaging. Consequently, by overexpressing circ_0011129 in hADSCs, we successfully loaded it into 3D-sEVs (3D-circ-sEVs) and its protective effect was better. Our studies provide a novel approach to preventing skin photoaging, which has important clinical significance and application value for the development of non-coding RNA drugs to treat skin photoaging. We first screened out hADSCs-derived sEVs with excellent anti-oxidant effects. We then compared the sEVs collected from traditional 2D culture with 3D bioreactor culture. By miRNA-seq and GEO data analysis, we found that miRNAs in 3D-sEVs were enriched in cell activities related to apoptosis, cellular senescence, and inflammation. Subsequently, we prepared circ_0011129-loaded 3D-sEVs (3D-circ-sEVs) by overexpressing it in hADSCs for the treatment of photoaging in vitro. We proved that 3D-circ-sEVs can interfere with the process of cell photoaging and protect cells from UVA radiation damage, as well as in a H2O2-induced oxidative stress model.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Circ_0011129 Encapsulated by the Small Extracellular Vesicles Derived from Human Stem Cells Ameliorate Skin Photoaging

Zhang

Yao

et al. 2022

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

“…The candidate miRNAs and circRNAs interacting with CTSD mRNA were predicted with Arraystar homemade miRNA target prediction software based on TargetScan & miRanda. Also, differentially expressed circRNAs ( p ‐value < .05 and absolute fold‐change ≥ 1.5) were derived from our circRNA microarray dataset using the limma package in the R software 14 . The potential circRNAs associated with CTSD expression in photoaged fibroblasts were selected through the intersection of predicted circRNAs and our circRNA microarray profiles.…”

Section: Methodsmentioning

confidence: 99%

“…Nonetheless, the role of circRNAs in photoaging remains elusive. Our previous study has revealed an altered circRNA expression pattern in UVA‐induced photoaged dermal fibroblasts through performing the circRNA microarray analysis 14 . Which circRNA can regulate CTSD expression in photoaged fibroblasts is unknown.…”

Section: Introductionmentioning

confidence: 99%

CircRNA‐406918 enhances the degradation of advanced glycation end products in photoaged human dermal fibroblasts via targeting cathepsin D

Wang

Lan

et al. 2023

Photoderm Photoimm Photomed

Self Cite

View full text Add to dashboard Cite

BackgroundLysosomal cathepsin D (CTSD) can degrade internalized advanced glycation end products (AGEs) in dermal fibroblasts. CTSD expression is decreased in photoaged fibroblasts, which contributes to intracellular AGEs deposition and further plays a role in AGEs accumulation of photoaged skin. The mechanism under downregulated CTSD expression is unclear.ObjectiveTo explore possible mechanism of regulating CTSD expression in photoaged fibroblasts.MethodsDermal fibroblasts were induced into photoaging with repetitive ultraviolet A (UVA) irradiation. The competing endogenous RNA (ceRNA) networks were constructed to predict candidate circRNAs or miRNAs related with CTSD expression. AGEs‐BSA degradation by fibroblasts was studied with flow cytometry, ELISA, and confocal microscopy. Effects of overexpressing circRNA‐406918 via lentiviral transduction on CTSD expression, autophagy, AGE‐BSA degradation were analyzed in photoaged fibroblasts. The correlation between circRNA‐406918 and CTSD expression or AGEs accumulation in sun‐exposed and sun‐protected skin was studied.ResultsCTSD expression, autophagy, and AGEs‐BSA degradation were significantly decreased in photoaged fibroblasts. CircRNA‐406918 was identified to regulate CTSD expression, autophagy, and senescence in photoaged fibroblasts. Overexpressing circRNA‐406918 potently decreased senescence and increased CTSD expression, autophagic flux, and AGEs‐BSA degradation in photoaged fibroblasts. Moreover, circRNA‐406918 level was positively correlated with CTSD mRNA expression and negatively associated with AGEs accumulation in photodamaged skin. Further, circRNA‐406918 was predicted to mediate CTSD expression through sponging eight miRNAs.ConclusionThese findings suggest that circRNA‐406918 regulates CTSD expression and AGEs degradation in UVA‐induced photoaged fibroblasts and might exert a role in AGEs accumulation in photoaged skin.

show abstract

Promotion of the genipin crosslinked chitosan-fiber hydrogel loaded with sustained release of clemastine fumarate in diabetic wound repair

Zuo

Shi

Jiang

et al. 2023

International Journal of Biological Macromolecules

View full text Add to dashboard Cite

Circular RNA expression profiles significantly altered in UVA‑irradiated human dermal fibroblasts

Cited by 5 publications

References 34 publications

Circ_0011129 Encapsulated by the Small Extracellular Vesicles Derived from Human Stem Cells Ameliorate Skin Photoaging

Circ_0011129 Encapsulated by the Small Extracellular Vesicles Derived from Human Stem Cells Ameliorate Skin Photoaging

CircRNA‐406918 enhances the degradation of advanced glycation end products in photoaged human dermal fibroblasts via targeting cathepsin D

Promotion of the genipin crosslinked chitosan-fiber hydrogel loaded with sustained release of clemastine fumarate in diabetic wound repair

Contact Info

Product

Resources

About