Circular RNA PVT1 promotes metastasis via miR-145 sponging in CRC

Wang, Zhongmiao; Shao, Meng; Xiang, Bowen; Zhao, Kai; Qin, Baoli

doi:10.1016/j.bbrc.2019.03.121

Cited by 77 publications

(68 citation statements)

References 20 publications

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“…In addition, a number of researches have expounded that circPVT1 could effectively sponge miR-125b, miR-145 and miR-497 to promote cell growth and metastasis in colorectal cancer and NSCLC [13,24,31]. Hence, we verified the correlation of circPVT1 and miR-199a-5p as well as the impact of circPVT1 and miR-199a-5p on human glioma cell lines.…”

Section: Discussionmentioning

confidence: 52%

RETRACTED ARTICLE: Silencing hsa_circ_PVT1 (circPVT1) suppresses the growth and metastasis of glioblastoma multiforme cells by up-regulation of miR-199a-5p

Chi

Yang

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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2020) Silencing hsa_circ_PVT1 (circPVT1) suppresses the growth and metastasis of glioblastoma multiforme cells by up-regulation of miR-ABSTRACT Background: Glioblastoma multiforme (GBM) is one of the most prevailing primary brain tumours among adults and most aggressive cancers. Despite multiple developments in medical and surgical treatments, GBM is still a deadly disease with a high mortality rate. Here, this study was performed to investigate the function of circPVT1 on GBM. Methods: CCK-8 and flow cytometry were utilised to estimate viability and apoptosis in both cells. qRT-PCR was performed to determine circPVT1 and miR-199a-5p expression. Western blot was conducted to determine apoptosis, migration and EMT-related proteins levels when silencing circPVT1. Subsequently, these parameters were re-tested after up-regulating miR-199a-5p. Results: CircPVT1 was highly expressed in GBM tissues. Silencing circPVT1 raised two cells apoptosis and reduced viability and migration capacity. Moreover, EGF-induced EMT was repressed by silencing circPVT1. In addition, miR-199a-5p expression was elevated when silencing circPVT1. And silencing circPVT1 exerted above changes via up-regulating miR-199a-5p. Finally, silencing circPVT1 repressed YAP1 and PI3K/AKT pathways via up-regulating miR-199a-5p. Conclusion: Our data suggested that silencing circPVT1 inhibited viability, migration, EGF-induced EMT and promoted apoptosis as well as repressed YAP1 and PI3K/AKT pathways by up-regulating miR-199a-5p. HIGHLIGHTS 1. CircPVT1 expression is highly expressed in GBM tissues; 2. Si-circPVT1 represses migration and promoted apoptosis in U539 and U251 cells; 3. Si-circPVT1 represses migration and promoted apoptosis when elevating miR-199a-5p; 4. Si-circPVT1 represses EGF-induced EMT when increasing miR-199a-5p; 5. Si-circPVT1 suppresses YAP1 and PI3K/AKT pathways by up-regulating miR-199-5p. ARTICLE HISTORY

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Section: Discussionmentioning

confidence: 52%

RETRACTED ARTICLE: Silencing hsa_circ_PVT1 (circPVT1) suppresses the growth and metastasis of glioblastoma multiforme cells by up-regulation of miR-199a-5p

Chi

Yang

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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“…For circ‐PVT1, it has been found dysregulated in some cancer cells, and a few studies emerge to display the role of circ‐PVT1 as an oncogene in various cancers . For example, circ‐PVT1 expression is elevated in gastric cancer cells compared to normal gastric epithelium cells, and is also increased in CRC cell lines compared to normal human colon epithelial cell line . As to the effect of circ‐PVT1 in cancer cells, a study displays that circ‐PVT1 downregulation inhibits cell proliferation, and induces cell apoptosis via suppressing the c‐Myc and Bcl‐2 expressions in ALL cells .…”

Section: Discussionmentioning

confidence: 99%

“…[9][10][11][12][13][14] For example, circ-PVT1 expression is elevated in gastric cancer cells compared to normal gastric epithelium cells, and is also increased in CRC cell lines compared to normal human colon epithelial cell line. 9,11 As to the effect of circ-PVT1 in cancer cells, a study displays that circ-PVT1 downregulation inhibits cell proliferation, and induces cell apoptosis via suppressing the c-Myc and Bcl-2 expressions in ALL cells. 10 Besides, a study shows that circ-PVT1 downregulation represses cell proliferation, while enhances cell apoptosis through negatively regulating miR-497, and reduces Bcl-2 expression in NSCLC cells.…”

Section: Cells; and (Ii)mentioning

confidence: 99%

“…Among the identified circRNA, circular RNA PVT1 (circ‐PVT1) is derived from the PVT1 locus, which is located on the well‐known cancer risk region (chromosome 8q24) . Circular RNA PVT1 (Circ‐PVT1) has been reported to promote cell proliferation, migration and invasion but suppress cell apoptosis in several cancers, such as nonsmall cell lung cancer (NSCLC), colorectal cancer (CRC) and head/neck squamous cell carcinoma, indicating that circ‐PVT1 might function as an oncogene in these cancers . In clinical practices, the abnormally overexpressed circ‐PVT1 has shown the potential to be a predictive factor for poor prognosis in patients with different malignancies as well .…”

Section: Introductionmentioning

confidence: 99%

“…[9][10][11][12][13] In clinical practices, the abnormally overexpressed circ-PVT1 has shown the potential to be a predictive factor for poor prognosis in patients with different malignancies as well. 9,11,14 Considering different cancers present with the common feature of malignant proliferation, we hypothesized that the functions of circ-PVT1 on promoting cancer cell proliferation and suppressing apoptosis might also be existed in EOC cells. As to EOC, several studies have revealed that PVT1 (wherein circ-PVT1 derives from) functions as an oncogene through promoting cell proliferation and reducing apoptosis in EOC cells.…”

Section: Introductionmentioning

confidence: 99%

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Circular RNA PVT1 enhances cell proliferation but inhibits apoptosis through sponging microRNA‐149 in epithelial ovarian cancer

Sun

Luo

Gao

2020

J of Obstet and Gynaecol

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Aim This study aimed to investigate the influence of circular RNA PVT1 (circ‐PVT1) on epithelial ovarian cancer (EOC) cell proliferation and apoptosis, more importantly, to identify the target microRNAs (miRNA) of circ‐PVT1 in EOC. Methods Circ‐PVT1 expression in EOC cell lines and nonmalignant control cells was detected. Cell proliferation, apoptosis and candidate target miRNA (miR‐149, miR‐183 and miR‐194) expressions were detected in circ‐PVT1 overexpression treated CAOV3 cells and circ‐PVT1 knock‐down treated SKOV3 cells. Furthermore, miR‐149 overexpression and miR‐149 knock‐down plasmids were transfected into circ‐PVT1 dysregulated CAOV3 cells and SKOV3 cells, respectively, and cell proliferation as well as apoptosis were detected. Results Circ‐PVT1 expression was increased in human EOC cell lines (CAOV3, SKOV3, SNU119 and OVCAR3) compared to human normal ovary surface epithelial cell line (HOSEpiC). In SKOV3 cells, cell proliferation was reduced at 48 and 72 h but cell apoptosis rate was increased at 48 h by circ‐PVT1 knock‐down. In CAOV3 cells, cell proliferation was increased at 48 and 72 h but cell apoptosis rate was decreased at 48 h by circ‐PVT1 overexpression. Besides, circ‐PVT1 negatively regulated miR‐149 but not miR‐183 or miR‐194 in SKOV3 and CAOV3 cells. Rescue experiments showed that miR‐149 knock‐down increased cell proliferation but decreased apoptosis in circ‐PVT1 knock‐down treated SKOV3 cells, while miR‐149 overexpression reduced cell proliferation but enhanced apoptosis in circ‐PVT1 overexpression treated CAOV3 cells. Conclusion Circ‐PVT1 enhances cell proliferation but inhibits cell apoptosis through sponging miR‐149 in EOC cells, which suggests that circ‐PVT1 may serve as a treatment target in EOC.

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Circular RNA: A promising new star for the diagnosis and treatment of colorectal cancer

Zhang

Sun

et al. 2021

Cancer Medicine

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Colorectal cancer (CRC) is one of the most common malignant tumors of the digestive tract, and morbidity and mortality rank third and second in malignant tumors. [1][2][3] Currently, early screening for CRC can effectively improve the survival rate of patients with CRC, but approximately 25% of CRC patients develop metastatic disease from an early stage. [4][5][6] Therefore, a promising field of antimetastatic therapy lies in the targeted inhibition of cancer cells with high metastatic potential from the primary site. 7,8 Recently, there has been a qualitative leap in tumor medical technology, such as the application of laparoscopic surgery, nano-assembly technology, PET/ CT imaging technology, and dynamic network biomarker (DNB), etc. 9-11 However, the cure rate and survival rate of CRC are still very low, mainly lacking new drug treatment targets and biomarkers for the treatment of CRC. 12 According to the research of circular RNAs in the CRC field, circular RNAs are expected to be therapeutic targets

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Circular RNA PVT1 promotes metastasis via miR-145 sponging in CRC

Cited by 77 publications

References 20 publications

RETRACTED ARTICLE: Silencing hsa_circ_PVT1 (circPVT1) suppresses the growth and metastasis of glioblastoma multiforme cells by up-regulation of miR-199a-5p

RETRACTED ARTICLE: Silencing hsa_circ_PVT1 (circPVT1) suppresses the growth and metastasis of glioblastoma multiforme cells by up-regulation of miR-199a-5p

Circular RNA PVT1 enhances cell proliferation but inhibits apoptosis through sponging microRNA‐149 in epithelial ovarian cancer

Circular RNA: A promising new star for the diagnosis and treatment of colorectal cancer

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