Circular RNA ROCK1, a novel circRNA, suppresses osteosarcoma proliferation and migration via altering the miR-532-5p/PTEN axis

Liu, Yize; Qiu, Guanzhen; Luo, Yinzhou; Li, Shanshan; Xu, Yeqiu; Zhang, Yuanzhuang; Hu, Jian; Li, Peifeng; Pan, Hai; Wang, Yong

doi:10.1038/s12276-022-00806-z

Cited by 27 publications

(19 citation statements)

References 52 publications

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“…For example, the circular RNA ciRs7 can adsorb miR-7 to relieve the repression of its target genes [ 12 , 13 ]. circROCK1-E3/E4 suppresses tumor growth and metastasis by upregulating PTEN through miR-532-5p sponging [ 14 ]. Analogously, circ_001678 can sponge miR-326 to increase ZEB1 expression and promote immune escape in NSCLC [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

circRNF10 Regulates Tumorigenic Properties and Natural Killer Cell-Mediated Cytotoxicity against Breast Cancer through the miR-934/PTEN/PI3k-Akt Axis

Liu

Yang

Zheng

et al. 2022

Cancers

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Circular RNA (circRNA), a type of non-coding RNA, has received a great deal of attention with regard to the initiation and progression of tumors. However, the molecular mechanism and function of circRNAs in breast cancer (BC) remain unclear. In the current study, we discovered that hsa_circ_0028899 (also called circRNF10) was significantly reduced in BC tissues, and a higher level of circRNF10 was markedly related to a favorable prognosis. The results of CCK8, colony formation, Transwell, ELISA, and NK cell-mediated cytotoxicity assays indicated that increased circRNF10 expression could significantly repress the proliferation, invasion, and migration of BC cells and enhance the killing efficiency of NK cells against BC cells. According to these biological functions, the possible role and molecular mechanism of circRNF10 in BC cells were further investigated. We used bioinformatics prediction tools to predict circRNF10-bound miRNAs, which were verified by many experimental studies, including FISH, luciferase reporter assays, RIP, and Western blots. These data suggest that circRNF10 serves as a molecular sponge for miR-934 to further regulate PTEN expression and PI3k/Akt/MICA signaling in vitro and tumor growth in vivo. Altogether, these findings reveal that circRNF10 functions as a novel anti-oncogene in BC via sponging miR-934 and suppressing the PI3K/Akt/MICA pathway.

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Section: Introductionmentioning

confidence: 99%

circRNF10 Regulates Tumorigenic Properties and Natural Killer Cell-Mediated Cytotoxicity against Breast Cancer through the miR-934/PTEN/PI3k-Akt Axis

Liu

Yang

Zheng

et al. 2022

Cancers

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“…The secondary structure of pre-mRNAs brings forming exons of circRNA into close proximity and consequently promotes circRNA biogenesis. A previous study indicated that QKI targets the introns flanking the circRNA-forming exons of ROCK1 and NOTCH1 to promote cyclization [ 38 , 39 ]. Consistent with these studies, we found that circCABIN1 has binding sites located in introns 24 and 29 of CABIN1 pre-mRNA that circCABIN1 biogenesis is enhanced via binding to recognition elements within introns in the vicinity of splice sites associated with circCABIN1 formation.…”

Section: Discussionmentioning

confidence: 99%

Exosome-transmitted circCABIN1 promotes temozolomide resistance in glioblastoma via sustaining ErbB downstream signaling

et al. 2023

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Although temozolomide (TMZ) provides significant clinical benefit for glioblastoma (GBM), responses are limited by the emergence of acquired resistance. Here, we demonstrate that exosomal circCABIN1 secreted from TMZ-resistant cells was packaged into exosomes and then disseminated TMZ resistance of receipt cells. CircCABIN1 could be cyclized by eukaryotic translation initiation factor 4A3 (EIF4A3) and is highly expressed in GBM tissues and glioma stem cells (GSCs). CircCABIN1 is required for the self-renewal maintenance of GSCs to initiate acquired resistance. Mechanistically, circCABIN1 regulated the expression of olfactomedin-like 3 (OLFML3) by sponging miR-637. Moreover, upregulation of OLFML3 activating the ErbB signaling pathway and ultimately contributing to stemness reprogramming and TMZ resistance. Treatment of GBM orthotopic mice xenografts with engineered exosomes targeting circCABIN1 and OLFML3 provided prominent targetability and had significantly improved antitumor activity of TMZ. In summary, our work proposed a novel mechanism for drug resistance transmission in GBM and provided evidence that engineered exosomes are a promising clinical tool for cancer prevention and therapy. Graphical Abstract

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“…The study of Liu et al 31 showed that circROCK1-E3/E4, produced from exons 3 and 4 of ROCK1 precursor mRNA, was downregulated in patients with osteosarcoma and associated with poor prognosis. Downregulation of circROCK1-E3/E4 promoted proliferation and migration in U2OS and HOS cells.…”

Section: The Role and Regulatory Mechanism Of Circular Rna In The Occ...mentioning

confidence: 99%

Recent Advances of Circular RNAs as Biomarkers for Osteosarcoma

Wu¹,

Zheng²,

He³

et al. 2023

IJGM

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Osteosarcoma is the most common primary malignant bone tumor in young adult, which is prone to early metastasis and poor prognosis. The current treatment methods need to be improved. Circular RNA is a covalently blocked circular, non-coding RNA that plays an essential role in the occurrence, development, clinical diagnosis, and treatment of various diseases. Recently, an increasing number of circRNAs have been identified in osteosarcoma. Understanding its role in osteosarcoma is conducive to the early detection, diagnosis, and treatment of osteosarcoma. In this paper, we reviewed the mechanism of action of circular RNA in the occurrence and development of osteosarcoma and its clinical application in recent years.

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Circular RNA ROCK1, a novel circRNA, suppresses osteosarcoma proliferation and migration via altering the miR-532-5p/PTEN axis

Cited by 27 publications

References 52 publications

circRNF10 Regulates Tumorigenic Properties and Natural Killer Cell-Mediated Cytotoxicity against Breast Cancer through the miR-934/PTEN/PI3k-Akt Axis

circRNF10 Regulates Tumorigenic Properties and Natural Killer Cell-Mediated Cytotoxicity against Breast Cancer through the miR-934/PTEN/PI3k-Akt Axis

Exosome-transmitted circCABIN1 promotes temozolomide resistance in glioblastoma via sustaining ErbB downstream signaling

Recent Advances of Circular RNAs as Biomarkers for Osteosarcoma

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