Circular RNAs Regulate Glucose Metabolism in Cancer Cells

Ji, Xiaoyu; Sun, Wei; Lv, Chengzhou; Huang, Jiapeng; Zhang, Hao

doi:10.2147/ott.s316597

Cited by 9 publications

(9 citation statements)

References 191 publications

(109 reference statements)

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“…Through a series of experiments in PTC cells, circPI4KA knockdown was manifested to suppress cell proliferation and motility. Glucose metabolism can provide energy for tumor growth and circRNAs have participated in glucose metabolism of human cancer cells [25]. Cir-cRAD18 silencing affected glucose uptake and lactate production in PTC cell progression [26].…”

Section: Discussionmentioning

confidence: 99%

CircPI4KA Overexpression Enhances Carcinogenesis and Glycolysis Metabolism in Papillary Thyroid Carcinoma by Causing the miR-1287-5p-Mediated NRP2 Expression Elevation

Sun,

Xu,

You

2023

Horm Metab Res

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Circular RNAs (circRNAs) are implicated in regulating the pathogenesis of papillary thyroid carcinoma (PTC). Herein, we aimed to investigate how circRNA phosphatidylinositol 4-kinase IIIα (circPI4KA, hsa_circ_0062389) functioned as an oncogene in PTC. CircPI4KA, microRNA-1287-5p (miR-1287-5p) and Neuropilin-2 (NRP2) level detection were completed by reverse transcription-quantitative polymerase chain reaction assay. Cell proliferation was assessed through Cell Counting Kit-8 assay, colony formation assay, and EdU assay. Transwell assay was used for detecting migration and invasion abilities. Cell migration was also determined by wound healing assay. Cell apoptosis was assessed using flow cytometry assay. The protein examination was performed using western blot. Glycolysis was evaluated via commercial kits. Dual-luciferase reporter assay and RNA immunoprecipitation assay were conducted for target analysis. The role of circPI4KA in vivo was explored and analyzed via tumor xenograft assay. CircPI4KA was significantly upregulated in PTC tissues and cells. Knockdown of circPI4KA suppressed proliferation, migration, invasion, glycolysis, and induced apoptosis of PTC cells. CircPI4KA interacted with miR-1287-5p in PTC cells. The antitumor function of circPI4KA downregulation was reversed by inhibition of miR-1287-5p. The miR-1287-5p directly targeted NRP2, and circPI4KA elevated the NRP2 expression by sponging miR-1287-5p. PTC progression was impeded by miR-1287-5p via targeting NRP2. Silencing circPI4KA inhibited tumor growth in vivo through the miR-1287-5p/NRP2 axis. The collective results revealed that circPI4KA induced the upregulation of NRP2 via sponging miR-1287-5p, thus acting as a carcinogenic factor in PTC.

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Section: Discussionmentioning

confidence: 99%

CircPI4KA Overexpression Enhances Carcinogenesis and Glycolysis Metabolism in Papillary Thyroid Carcinoma by Causing the miR-1287-5p-Mediated NRP2 Expression Elevation

Sun,

Xu,

You

2023

Horm Metab Res

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“…Наконец, гликолиз обеспечивает множество промежуточных продуктов обмена веществ и питательных веществ, которые могут быть использованы в качестве сырья для синтеза важнейших биологических макромолекул (жирных кислот, нуклеиновых кислот, аминокислот) [264]. Предыдущие исследования показали, что эффект Варбурга может регулироваться онкогенами, генами-супрессорами опухолей и ключевыми ферментами метаболизма глюкозы, но этим не исчерпиваются основные механизмы регуляции [160,176,303]. Результаты исследований последних 5 лет показывают, что кольцевые РНК являются новым важным регуляторным фактором эффекта Варбурга (рис.…”

Section: кольцевые рнк в регуляции апоптоза клеток при лечении больны...unclassified

“…Результаты исследований последних 5 лет показывают, что кольцевые РНК являются новым важным регуляторным фактором эффекта Варбурга (рис. 7) [150,163,318].…”

Section: кольцевые рнк в регуляции апоптоза клеток при лечении больны...unclassified

“…По завершении процесса гликолиза молочная кислота высвобождается во внеклеточный матрикс. В последние годы исследования ферментов, регулирующих перепрограммирование метаболизма глюкозы при раке, активизировались, и было обнаружено, что кольцевые РНК оказывают существенное влияние на трансформацию эффекта Варбурга [163,240]. енолаза 1, как основной фермент гликолиза, играет свою особую регулирующую роль в эффекте Варбурга опухолевых клеток.…”

Section: кольцевые рнк и регуляция отдельных ферментов гликолизаunclassified

“…Консервативная последовательность структуры кольцевых РНК уже описана для отдельных видов растений, животных и даже архей [113,156,259,388]. Показано, что консервативная последовательность кольцевых РНК формируется из различных генов: PVT1, HIPK2, IPW, KIAA0182 [160,161,296]. Применив методы биоинформационного анализа, исследователи обнаружили консервативную последовательность кольцевой РНК circMBNL1 в клетках дрозофилы и в клетках мозга людей [158,299].…”

Section: Introductionunclassified

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Circular RNAs in eukaryotic cells: origin, characteristics, mechanisms of molecular functioning in human malignant diseases

Vashchenko

Chuklovin

Shabanov

2023

Rev Clin Pharm Drug Ther

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Circular RNAs (circRNAs) are an evolutionarily conserved novel class of non-coding endogenous RNAs (ncRNAs) found in the eukaryotic transcriptome, originally believed to be aberrant RNA splicing by-products with limited functionality. However, recent advances in highthroughput genomic technology have allowed circRNAs to be characterized in detail and revealed their important functions in controlling various biological and molecular processes, the most essential being gene regulation. Due to structural stability, high expression, availability of microRNA (miRNA) binding sites and tissue-specific expression, circRNAs have become hot topic of research in RNA 2 biology. Unlike linear RNAs, circRNAs are produced differentially by backsplicing exons or lariat introns from a pre-messenger RNA (mRNA) forming covalently closed loop-like molecules missing 3' poly-(A) tail or 5' cap structures, thus rendering them resistant to exonuclease-mediated degradation. Previous studies have revealed multiple roles of circRNAs as sponges for miRNA and RNA-binding proteins (RBP), as well as regulators of transcription, translation, and splicing events. Recent advances in the field suggest that the circRNAs are involved in many human disorders, including cancer and neurodegenerative disorders such as Alzheimers and Parkinsons disease, due to their aberrant expression in different pathological conditions. The circRNAs are stable in cells, owing to their circular structure. Participation of circRNAs in programmed cellular destruction by autophagy is discussed in details. The autophagy is a catabolic process which promotes decomposition and recycling of harmful or redundant biological macromolecules and initiates destruction of ageing cells. Processes how circRNAs influence a course of a disease, including an autophagy are in detail discussed, specifying that it joins at the beginning and upon development of various illnesses, and it can influence drug resistance (for example, antitumor efficiency of Cisplatin). The functional versatility exhibited by circRNAs enables them to serve as potential diagnostic or predictive biomarkers for various diseases. This review discusses the properties, characterization, profiling, and the diverse molecular actions of circRNAs and their usage as potential therapeutic targets in different human malignancies.

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Inhibition of glycolysis and SIRT1/GLUT1 signaling ameliorates the apoptotic effect of Leptosidin in prostate cancer cells

Park,

Lee,

Sim

et al. 2024

Phytotherapy Research

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Since the silent information regulation 2 homolog‐1 (sirtuin, SIRT1) and glucose transporter 1 (GLUT1) are known to modulate cancer cell metabolism and proliferation, the role of SIRT1/GLUT1 signaling was investigated in the apoptotic effect of Leptosidin from Coreopsis grandiflora in DU145 and PC3 human prostate cancer (PCa) cells. The 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) assay, cell cycle analysis, Western blotting, cBioportal correlation analysis, and co‐immunoprecipitation were used in this work. Leptosidin showed cytotoxicity, augmented sub‐G1 population, and abrogated the expression of pro‐poly (ADP‐ribose) polymerase (pro‐PARP) and pro‐cysteine aspartyl‐specific protease (pro‐caspase3) in DU145 and PC3 cells. Also, Leptosidin inhibited the expression of SIRT1, GLUT1, pyruvate kinase isozymes M2 (PKM2), Hexokinase 2 (HK2), and lactate dehydrogenase A (LDHA) in DU145 and PC3 cells along with disrupted binding of SIRT1 and GLUT1. Consistently, Leptosidin curtailed lactate, glucose, and ATP in DU145 and PC3 cells. Furthermore, SIRT1 depletion enhanced the decrease of GLUT1, LDHA, and pro‐Cas3 by Leptosidin in treated DU145 cells, while pyruvate suppressed the ability of Leptosidin in DU145 cells. These findings suggest that Leptosidin induces apoptosis via inhibition of glycolysis and SIRT1/GLUT1 signaling axis in PCa cells.

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Circular RNAs Regulate Glucose Metabolism in Cancer Cells

Cited by 9 publications

References 191 publications

CircPI4KA Overexpression Enhances Carcinogenesis and Glycolysis Metabolism in Papillary Thyroid Carcinoma by Causing the miR-1287-5p-Mediated NRP2 Expression Elevation

CircPI4KA Overexpression Enhances Carcinogenesis and Glycolysis Metabolism in Papillary Thyroid Carcinoma by Causing the miR-1287-5p-Mediated NRP2 Expression Elevation

Circular RNAs in eukaryotic cells: origin, characteristics, mechanisms of molecular functioning in human malignant diseases

Inhibition of glycolysis and SIRT1/GLUT1 signaling ameliorates the apoptotic effect of Leptosidin in prostate cancer cells

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