Circular RNAs: Regulatory functions in respiratory tract cancers

Peng, Ziyi; Fang, Shuai; Jiang, Meina; Zhao, Xiaodong; Zhou, Chengwei; Gong, Zhaohui

doi:10.1016/j.cca.2020.07.042

Cited by 6 publications

(5 citation statements)

References 68 publications

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“…They usually bind to miRNAs and regulate the expression of miRNA target gene transcripts that harbor the same miRNA-binding sites, constructing a complex post-transcriptional regulatory network. According to Peng et al, 11 upregulated circRNAs and 2 downregulated circRNAs were identified in LUAD [55]. These circRNAs have been demonstrated to be associated with the migration, invasion, apoptosis, and other aspects of tumor progression, and they serve as biomarkers for the diagnosis or prognosis of LUAD [20][21][22][23][24][25][26][27][28][29][30][31].…”

Section: Discussionmentioning

confidence: 99%

circ_0000567/miR-421/TMEM100 Axis Promotes the Migration and Invasion of Lung Adenocarcinoma and Is Associated with Prognosis

Yang¹,

Si²,

Xiao³

et al. 2022

J. Cancer

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Purpose: Due to the high metastatic ability and poor prognosis of lung adenocarcinoma (LUAD), we identified novel non-coding RNAs, which constitute approximately 60% of human transcripts, as prognostic biomarkers and potential therapeutic targets for LUAD. Methods: In this study, we downloaded and analyzed microRNA (miRNA) datasets from The Cancer Genome Atlas (TCGA) to identify dysregulated miRNAs correlating with the overall survival (OS) of LUAD patients. miR-421, circ_0000567, and TMEM1000 expression levels were examined by quantitative real-time polymerase chain reaction (qRT-PCR) in NSCLC tissues from 73 patients and adjacent normal tissues. Cell migration and invasion were assayed using wound healing and transwell assays. miR-421 target predictions were conducted using starBase, CircInteractome, circBank, TargetScan, miRanda, MirDB, miRpath, and Gene Expression Omnibus (GEO) databases. The circular structure and stability of circ_0000567 were verified by RNase R digestion and qRT-PCR using oligo(dT) and random primers. A luciferase reporter assay was used to evaluate the relationship between miR-421, circ_0000567, and TMEM100. Results: The miRNA panel associated with OS in patients with LUAD was screened according to the hazard ratio (HR) of miRNAs from high to low. Based on the correlation between these miRNAs and OS, as well as miRNA expression levels, miR-421 was selected for further outcome analysis. High miR-421 expression was an independent risk factor for shorter OS in 73 patients collected from our department. Bioinformatic analyses, luciferase reporter assays, and functional assays showed that circ_0000567 could act as a sponge for miR-421 and prevent it from directly targeting the 3'-untranslated region of TMEM100 mRNA and further degrading it in LUAD. miR-421 expression was significantly upregulated, while circ_0000567 and TMEM100 were downregulated in tumor tissues of LUAD, compared to their counterparts in normal tissues. Gain-and loss-of-function assays showed that miR-421 promoted LUAD cell migration and invasion. Overexpression of circ_0000567 inhibited migration and invasion, whereas co-transfection of circ_0000567 and miR-421 mimics partly counteracted this effect. TMEM1000 was upregulated by enhanced circ_0000567 in LUAD cells, and the expression of TMEM1000 was inversely proportional to miR-421, whereas it was directly proportional to circ_0000567 in 73 LUAD specimens, which confirmed the competitive endogenous RNA (ceRNA) network. Conclusion: Our findings suggest that miR-421 promotes the migration and invasion of lung adenocarcinoma via circ_0000567/miR-421/TMEM100 signaling and could be a prognostic biomarker for LUAD.

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Section: Discussionmentioning

confidence: 99%

circ_0000567/miR-421/TMEM100 Axis Promotes the Migration and Invasion of Lung Adenocarcinoma and Is Associated with Prognosis

Yang¹,

Si²,

Xiao³

et al. 2022

J. Cancer

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“…ceRNA network and tumors. A number of studies have demonstrated the role of circRNAs in respiratory system (40), digestive system (41,42) and female reproductive system tumors (43,44). circRNAs can be upregulated or downregulated in tumors, which regulates mRNA expression in tumor cells by regulating miRNA.…”

Section: Cernamentioning

confidence: 99%

Role of circular RNA as competing endogenous RNA in ovarian cancer (Review)

Ye,

Xiang,

Wang

et al. 2024

Int J Mol Med

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Circular RNA (circRNA), a type of non-coding RNA, plays a regulatory role in biological processes. The special loop structure of circRNA makes it highly stable and specific in diseased tissues and cells, especially in tumors. Competing endogenous RNAs (ceRNAs) compete for the binding of microRNA (miRNA) at specific binding sites and thus regulate gene expression. ceRNAs play an important role in various diseases and are currently recognized as the most prominent mechanism of action of circRNAs. circRNAs can modulate the proliferation, migration, invasion and apoptosis of tumor cells through the ceRNA mechanism. With further research, circRNAs may serve as novel markers and therapeutic targets for ovarian cancer (OC). In the present review, the research progress of circRNAs as ceRNAs in OC was summarized, focusing on the effects of the circRNA/miRNA/mRNA axis on the biological functions of OC cells through mediating pivotal signaling pathways. The role of circRNAs in the diagnosis, prognostic assessment and treatment of OC was also discussed in the present review.

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“…Another study found that the circRNA circ_0067934 promotes the progression of pancreatic cancer by sponging miR-1324 and activating the Wnt/β-catenin signaling pathway [113] . In addition, the circRNA circ_0008450 promotes the spread and invasion of gastric cancer cells by sponging miR-422a and activating the expression of the oncogene SOX4 [72] . These studies highlight the potential of circRNAs as diagnostic and prognostic markers and emphasize therapeutic targets for cancer treatment.…”

Section: Deciphering the Diversity Of Non-coding Rnas In Cancermentioning

confidence: 99%

Unlocking the potential of non-coding RNAs in cancer research and therapy

Uppaluri,

Challa,

Gaur

et al. 2023

Translational Oncology

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Circular RNAs: Regulatory functions in respiratory tract cancers

Cited by 6 publications

References 68 publications

circ_0000567/miR-421/TMEM100 Axis Promotes the Migration and Invasion of Lung Adenocarcinoma and Is Associated with Prognosis

circ_0000567/miR-421/TMEM100 Axis Promotes the Migration and Invasion of Lung Adenocarcinoma and Is Associated with Prognosis

Role of circular RNA as competing endogenous RNA in ovarian cancer (Review)

Unlocking the potential of non-coding RNAs in cancer research and therapy

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