Circulating anodic antigen levels in serum before and after chemotherapy with praziquantel in schistosomiasis mansoni

Jonge, Niels de; Caluwe, P. De; Hilberath, G. W.; Krijger, F. W.; Polderman, A.M.; Deelder, A. M.

doi:10.1016/0035-9203(89)90507-5

Cited by 73 publications

(38 citation statements)

References 16 publications

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“…The test usually becomes negative within 1 week of successful anti-schistosomal therapy. 15,16 CAA testing was performed at the NIMR laboratory in Mwanza using the upconverting phosphor (UCP) technology lateral flow assay as previously described. 17,18 We used the strategy recommended by the WHO/the Special Programme for Research and Training in Tropical Diseases to define a composite reference standard for schistosome infection as the presence of schistosome eggs visualized microscopically and/or a serum CAA value ≥ 30 pg/mL.…”

Section: Methodsmentioning

confidence: 99%

Schistosomiasis and Human Immunodeficiency Virus in Men in Tanzania

Downs

Dood

Dee

et al. 2017

The American Society of Tropical Medicine and Hygiene

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Abstract. Schistosomiasis is a parasitic worm infection that affects over 260 million individuals worldwide. Women with schistosome infections have been demonstrated to have a 4-fold increase in the odds of human immunodeficiency virus (HIV) infection compared with women without schistosome infections. A relationship between schistosome and HIV infections has not been clearly defined in men. Among 674 men aged 18-50 years living in rural Tanzania, we identified 429 (63.6%) who had a schistosome infection as defined by serum positivity for schistosome circulating anodic antigen, visualization of parasite eggs in urine or stool, or both. HIV infection was identified in 38 (5.6%). The odds of HIV infection was 1.3 [95% confidence interval = 0.6-2.5] (P = 0.53) among men with any schistosome infection (Schistosoma haematobium or Schistosoma mansoni), and it was 1.4 [0.6-3.3] (P = 0.43) among men with S. haematobium infection. Men with S. haematobium infection were significantly more likely to report the symptom of hemospermia than men without S. haematobium infection. We conclude that schistosome infections appear to have little to no association with HIV infection in men.

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Section: Methodsmentioning

confidence: 99%

Schistosomiasis and Human Immunodeficiency Virus in Men in Tanzania

Downs

Dood

Dee

et al. 2017

The American Society of Tropical Medicine and Hygiene

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“…17,18 The excretion of the schistosome-circulating antigens in the urine of infected patients makes possible the use of noninvasive techniques for simple diagnosis of schistosomiasis. [19][20][21][22] In addition, low fluctuations of urinary elimination of the circulating antigens have been observed. 23,24 Attallah and others 5 examined the presence of a 74-kD circulating antigen in the urine of infected and uninfected cases using Fast Dot-ELISA based on MAb BRL4.…”

Section: Discussionmentioning

confidence: 99%

Immunochemical characterization and diagnostic potential of a 63-kilodalton Schistosoma antigen.

Attallah

Yones²,

Ismail³

et al. 1999

The American Journal of Tropical Medicine and Hygiene

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Abstract. Schistosoma circulating antigens were used for the detection of active infection. Anti-S. mansoni IgG2a monoclonal antibody (MAb) designated C5C4 was generated. The target epitope of this MAb was detected in adult worms, eggs, and cercariae antigenic extracts of S. mansoni and S. haematobium, had a molecular size of 63 kD, and was not detected in Fasciola hepatica and Ascaris. In addition, a 50-kD degradation product was identified only in the urine of infected individuals. Analysis by high-performance liquid chromatography of the purified antigen demonstrated only one peak. The 63-kD antigen was characterized as a protein containing 40.4% hydrophobic, 7.5% acidic, and 8.8% basic amino acids. The C5C4 MAb was used in a Fast Dot-ELISA for rapid and simple diagnosis of human schistosomiasis. The 63-kD circulating antigen was detected in 92% of urine samples from 330 S. mansoniinfected individuals, with 16% false-positive results among 130 noninfected individuals.The identification and characterization of schistosome antigens in different developmental stages and the assessment of their role in host-parasite interactions are needed as a step toward the diagnosis of human schistosomiasis. 1 Several investigators have isolated and characterized many of the schistosomiasis antigens in different developmental stages of the parasite that have a potential application in immunodiagnosis. [2][3][4] In the present study, we have identified and characterized a schistosome antigen using a specific anti-Schistosoma mansoni mouse monoclonal antibody (MAb) and evaluated the suitability of this MAb in the immunodiagnosis of S. mansoni infection. MATERIALS AND METHODSMonoclonal antibody subtype and isotype determination. A hybridoma cell line designated C5C4 was produced from mice infected with S. mansoni as described by Attallah and others. 5 An ELISA was used to characterize the anti-S. mansoni MAb (C5C4). A microliter plate (Costar, Cambridge, MA) was coated with 50 g/ml of this MAb in 0.05 M carbonate/bicarbonate buffer, pH 9.6. After blocking with 200 l/well of 0.3% nonfat milk, a 1:500 dilution of mouse IgG1, IgG2a, IgG2b, and IgG3 MAbs produced in sheep (The Binding Site, Birmingham, United Kingdom) were added and incubated at 37ЊC for 2 hr. Sheep anti-mouse IgGalkaline phosphatase conjugate (Sigma, St. Louis, MO) was added at a dilution of 1:1,000 for 1 hr at 37ЊC. Fifty microliters per well (1 mg/ml) of p-nitrophenyl phosphate in 0.1 M glycine buffer, pH 10.4, were added and the plate was incubated for 20 min at 37ЊC. The reaction was stopped with 3 N NaOH and the optical density was read at 405 nm using an EL 311 microplate autoreader (Bio-Tek Instruments, Winooski, VT).Preparation of antigenic extracts. The S. mansoni and S. haematobium antigenic extracts of worms (SWAP), cercariae (CAP), and eggs (SEA) were prepared according to the method of Da Silva and Ferri. 6 The same method was used for the preparation of Fasciola hepatica and Ascaris lumbricoides antigenic extracts. The protein content was determined u...

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“…In studies on populations living in endemic areas, the levels of these antigens were found to be related to the presence of an active infection; following successful treatment, CAA and CCA levels became negative within 3-6 weeks (De Jonge et al 1989;Van Lieshout et al 1994).…”

Section: Discussionmentioning

confidence: 99%

“…Antigen levels are related to intensity of infection, as expressed by parasite egg excretion. In studies with hospitalized patients CAA and CCA levels dropped to zero within 3-6 weeks after successful chemotherapy (De Jonge et al 1989;Van Lieshout et al 1991, 1993.…”

Section: Introductionmentioning

confidence: 99%

Detection of the circulating antigens CAA and CCA in a group of Dutch travellers with acute schistosomiasis

Lieshout

Polderman

Visser

et al. 1997

Tropical Med Int Health

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SummaryDetection of circulating anodic antigen (CAA) and circulating cathodic antigen (CCA) in serum and urine is a highly specific and sensitive alternative for the diagnosis of schistosome infections in endemic areas. However, it is not known how soon after the onset of infection these antigens can be detected in humans. Neither has there been much research on the detection of these antigens in individuals normally living in non-endemic areas. We studied the kinetics of CAA and CCA in serum and urine of a group of 28 Dutch tourists, shortly after accidental exposure to a Schistosoma infection during a visit to Mali. Twenty-seven were found to be positive for Schistosoma eggs and/or specific antibodies. From each individual, 1-4 serum samples were tested for circulating antigen level, 4-15 weeks after exposure, and urine samples were also tested from 22 subjects. CAA and CCA levels were quantified by monoclonal antibody-based ELISAs and TRIFMAs. In serum, 23 individuals (85%) were positive at least once for one or both antigens, but titres were generally very low. CAA and CCA could be detected 5 and 6 weeks after exposure, respectively. Urines were all found to be negative. Almost all cases were negative at 7 months' follow-up. keywords acute schistosomiasis, diagnosis, circulating antigens, CAA, CCA correspondence L. van Lieshout,

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Circulating anodic antigen levels in serum before and after chemotherapy with praziquantel in schistosomiasis mansoni

Cited by 73 publications

References 16 publications

Schistosomiasis and Human Immunodeficiency Virus in Men in Tanzania

Schistosomiasis and Human Immunodeficiency Virus in Men in Tanzania

Immunochemical characterization and diagnostic potential of a 63-kilodalton Schistosoma antigen.

Detection of the circulating antigens CAA and CCA in a group of Dutch travellers with acute schistosomiasis

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