Circulating Cell Free DNA and Citrullinated Histone H3 As Useful Biomarkers of NETosis In Endometrial Cancer.

Ronchetti, Livia; Terrenato, Irene; Corrado, Giacomo; Goeman, Frauke; Donzelli, Sara; Mandoj, Chiara; Merola, Roberta; Zampa, Ashanti; Carosi, Mariantonia; Blandino, Giovanni; Conti, Laura; Lobascio, Anna Maria; Iacobelli, Marcello; Vizza, Enrico; Piaggio, Giulia; Gurtner, Aymone

doi:10.21203/rs.3.rs-998151/v1

Cited by 1 publication

(1 citation statement)

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“…Our results showed that most NET formation pathway genes were down‐regulated after treatment. Among them, CitH3, a maker of NETosis (Ronchetti et al, 2022), was highly down‐regulated in fluctuating sites, but no differences were observed in the progressing samples. NETosis can be activated by a variety of receptors, all of which were down‐regulated in both progressing and fluctuating samples.…”

Section: Discussionmentioning

confidence: 99%

Subgingival host–microbiome metatranscriptomic changes following scaling and root planing in grade II/III periodontitis

Duran-Pinedo

Solbiati

Teles

et al. 2022

J Clinic Periodontology

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Aim To assess the effects of scaling and root planing (SRP) on the dynamics of gene expression by the host and the microbiome in subgingival plaque samples. Materials and Methods Fourteen periodontitis patients were closely monitored in the absence of periodontal treatment for 12 months. During this period, comprehensive periodontal examination and subgingival biofilm sample collection were performed bi‐monthly. After 12 months, clinical attachment level (CAL) data were compiled and analysed using linear mixed models (LMM) fitted to longitudinal CAL measurements for each tooth site. LMM classified the sites as stable (S), progressing (P), or fluctuating (F). After the 12‐month visit, subjects received SRP, and at 15 months they received comprehensive examination and supportive periodontal therapy. Those procedures were repeated at the 18‐month visit, when patients were also sampled. Each patient contributed with one S, one P, and one F site collected at the 12‐ and 18‐month visits. Samples were analysed using Dual RNA‐Sequencing to capture host and bacterial transcriptomes simultaneously. Results Microbiome and host response behaviour were specific to the site's progression classification (i.e., S, P, or F). Microbial profiles of pre‐ and post‐treatment samples exhibited specific microbiome changes, with progressing sites showing the most significant changes. Among them, Porphyromonas gingivalis was reduced after treatment, while Fusobacterium nucleatum showed an increase in proportion. Transcriptome analysis of the host response showed that interleukin (IL)‐17, TNF signalling pathways, and neutrophil extracellular trap formation were the primary immune response activities impacted by periodontal treatment. Conclusions SRP resulted in a significant “rewiring” of host and microbial activities in the progressing sites, while restructuring of the microbiome was minor in stable and fluctuating sites.

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Section: Discussionmentioning

confidence: 99%