Circulating endothelial progenitor cells are not affected by acute systemic inflammation

Abstract

Vascular injury causes acute systemic inflammation and mobilizes endothelial progenitor cells (EPCs) and endothelial cell (EC) colony-forming units (EC-CFUs). Whether such mobilization occurs as part of a nonspecific acute phase response or is a phenomenon specific to vascular injury remains unclear. We aimed to determine the effect of acute systemic inflammation on EPCs and EC-CFU mobilization in the absence of vascular injury. Salmonella typhus vaccination was used as a model of acute systemic inflammation. In a double-blind randomized crossover study, 12 healthy volunteers received S. typhus vaccination or placebo. Phenotypic EPC populations enumerated by flow cytometry [CD34+VEGF receptor (VEGF)R-2+CD133+, CD14+VEGFR-2+Tie2+, CD45−CD34+, as a surrogate for late outgrowth EPCs, and CD34+CXCR-4+], EC-CFUs, and serum cytokine concentrations (high sensitivity C-reactive protein, IL-6, and stromal-derived factor-1) were quantified during the first 7 days. Vaccination increased circulating leukocyte (9.8 ± 0.6 vs. 5.1 ± 0.2 × 109cells/l, P < 0.0001), serum IL-6 [0.95 (0–1.7) vs. 0 (0–0) ng/l, P = 0.016], and VEGF-A [60 (45–94) vs. 43 (21–64) pg/l, P = 0.006] concentrations at 6 h and serum high sensitivity C-reactive protein at 24 h [2.7 (1.4–3.6) vs. 0.4 (0.2–0.8) mg/l, P = 0.037]. Vaccination caused a 56.7 ± 7.6% increase in CD14+cells at 6 h ( P < 0.001) and a 22.4 ± 6.9% increase in CD34+cells at 7 days ( P = 0.04). EC-CFUs, putative vascular progenitors, and the serum stromal-derived factor-1 concentration were unaffected throughout the study period ( P > 0.05 for all). In conclusion, acute systemic inflammation causes nonspecific mobilization of hematopoietic progenitor cells, although it does not selectively mobilize putative vascular progenitors. We suggest that systemic inflammation is not the primary stimulus for EPC mobilization after acute vascular injury.

Endothelial Progenitor Cell Homing Decreases Postoperative Complications in Patients Undergoing Cardiac Surgery with Cardiopulmonary Bypass

“…Our results are consistent with recent reports that acute injuries induced by S. typhus vaccination injection (28) or acute hypoxia (29) do not cause immediate mobilization of vascular progenitors.…”

Extrarenal Progenitor Cells Do Not Contribute to Renal Endothelial Repair

“…This rare presence of circulating BM-derived EC-CFUs is also consistent with existing literature. 13,[32][33][34] To evaluate BM chimerism and provide a control for further transplantationinduced side effects, we decided to perform the assessment of the inflammatory response in parallel. Many BM-derived inflammatory cells (macrophages, neutrophils, DCs, B cells and T cells) were recruited to the kidney and this could be found to the same extent in all chimeric mice, demonstrating that the BM and BM originating cells were functional.…”

Endothelial progenitor cells, atheroma burden and clinical outcome in patients with coronary artery disease

“…Putative EPC and angiogenic monocytes were identified by flow cytometry and analysed as described previously17 using the following preconjugated antihuman monoclonal antibodies: anti-CD45-PercP (Becton Dickinson, UK), anti-CD34-FITC, anti-VEGFR-2-PE, anti-Tie-2-APC (R&D systems, USA), anti-CD-133-PE (Miltenyi Biotec, UK) and anti-CD14-FITC (Caltag Systems, UK) (figure 1). A total of 500 000 events were acquired in the leukocyte gate for each sample using a FACS-Calibur flow-cytometer (Becton Dickinson, UK).…”