Circulating extracellular vesicles and rheumatoid arthritis: a proteomic analysis

Alghamdi, Mansour A.; Alamry, Sultan Abdulmughni; Bahlas, Sami; Uversky, Vladimir N.; Redwan, Elrashdy M.

doi:10.1007/s00018-021-04020-4

Cited by 32 publications

(19 citation statements)

References 296 publications

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“…Numerous miRNAs can be encapsulated within cell-derived membranous structures called exosomes that can be transferred to neighboring or distant cells, thereby facilitating crosstalk between donor cells and recipient cells. 60 , 61 Exosomes are the most well-studied class of extracellular vesicles (EVs) and have an average diameter of 50–150 nm. 62 It has been well established that the transfer of metabolites and biological information between different cell types via exosomal miRNAs is crucial for the coordination of various physiological and pathological processes.…”

Section: Basic Biology Of Mirnasmentioning

confidence: 99%

“…Finally, ILVs are secreted into the extracellular space upon fusion of MVEs with the plasma membrane. 60 , 65 ILVs contain and carry complex biomolecules, including various types of proteins, lipids, and nucleic acids, such as DNA sequences, mRNAs, miRNAs, and other noncoding RNAs. Sorting and clustering of these exosomal cargoes into exosomes are largely dependent on particular endosomal sorting machineries.…”

Section: Basic Biology Of Mirnasmentioning

confidence: 99%

“…Growing data have revealed the tight association of exosomal miRNAs with RA pathogenesis. 60 In addition to being recognized as a novel class of endocrine factors for cellular self-regulation, miRNAs might also serve as paracrine messengers to establish intercellular communication in the context of RA. Recent studies exploring the cellular sources of exosomes in RA have focused on MSCs, synoviocytes, and leukocytes (Table 2 ).…”

Section: Roles Of Exosomal Mirnas In Ra Progressionmentioning

confidence: 99%

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Comprehensive overview of microRNA function in rheumatoid arthritis

Peng

Wang

et al. 2023

Bone Res

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MicroRNAs (miRNAs), a class of endogenous single-stranded short noncoding RNAs, have emerged as vital epigenetic regulators of both pathological and physiological processes in animals. They direct fundamental cellular pathways and processes by fine-tuning the expression of multiple genes at the posttranscriptional level. Growing evidence suggests that miRNAs are implicated in the onset and development of rheumatoid arthritis (RA). RA is a chronic inflammatory disease that mainly affects synovial joints. This common autoimmune disorder is characterized by a complex and multifaceted pathogenesis, and its morbidity, disability and mortality rates remain consistently high. More in-depth insights into the underlying mechanisms of RA are required to address unmet clinical needs and optimize treatment. Herein, we comprehensively review the deregulated miRNAs and impaired cellular functions in RA to shed light on several aspects of RA pathogenesis, with a focus on excessive inflammation, synovial hyperplasia and progressive joint damage. This review also provides promising targets for innovative therapies of RA. In addition, we discuss the regulatory roles and clinical potential of extracellular miRNAs in RA, highlighting their prospective applications as diagnostic and predictive biomarkers.

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Section: Basic Biology Of Mirnasmentioning

confidence: 99%

Section: Basic Biology Of Mirnasmentioning

confidence: 99%

Section: Roles Of Exosomal Mirnas In Ra Progressionmentioning

confidence: 99%

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Comprehensive overview of microRNA function in rheumatoid arthritis

Peng

Wang

et al. 2023

Bone Res

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“…In addition, it is worthy for modifying exosomes to improve their targeting effect on macrophages to achieve the precise regulation of M2 macrophages. Moreover, in addition to non-coding RNA, other bioactive substances in exosomes, such as specific components of lipids and proteins, also play an essential role in cellular communication, which are predicted to be new disease biomarkers [65,66]. Therefore, it is necessary to further explore the wider role of exosomes in tendon-bone healing after ACLR surgery.…”

Section: Discussionmentioning

confidence: 99%

BMSC-derived exosomes promote tendon-bone healing after anterior cruciate ligament reconstruction by regulating M1/M2 macrophage polarization in rats

Tong

et al. 2022

Stem Cell Res Ther

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Background Recent studies have shown that bone marrow stromal cell-derived exosomes (BMSC-Exos) can be used for tissue repair. However, whether the BMSC-Exos can promote tendon-bone healing after anterior cruciate ligament reconstruction (ACLR) is still unclear. In this study, we observed in vivo and in vitro the effect of rat BMSC-Exos on tendon-bone healing after ACLR and its possible mechanism. Methods Highly expressed miRNAs in rat BMSC-Exos were selected by bioinformatics and verified in vitro. The effect of overexpressed miRNA in BMSC-Exos on M2 macrophage polarization was observed. A rat model of ACLR was established. The experimental components were divided into three groups: the control group, the BMSC-Exos group, and the BMSC-Exos with miR-23a-3p overexpression (BMSC-Exos mimic) group. Biomechanical tests, micro-CT, and histological staining were performed for analysis. Results Bioinformatics analysis showed that miR-23a-3p was highly expressed in rat BMSC-Exos and could target interferon regulatory factor 1 (IRF1, a crucial regulator in M1 macrophage polarization). In vitro, compared with the control group or the BMSC-Exos group, the BMSC-Exos mimic more significantly promoted the polarization of macrophages from M1 to M2. In vivo, at 2 weeks, the number of M2 macrophages in the early local stage of ACLR was significantly increased in the BMSC-Exos mimic group; at 4 and 8 weeks, compared with the control group or the BMSC-Exos group, the bone tunnels of the tibia and femur sides of the rats in the BMSC-Exos mimic group were significantly smaller, the interface between the graft and the bone was narrowed, the bone volume/total volume ratio (BV/TV) increased, the collagen type II alpha 1 level increased, and the mechanical strength increased. Conclusions BMSC-Exos promoted M1 macrophage to M2 macrophage polarization via miR-23a-3p, reduced the early inflammatory reaction at the tendon-bone interface, and promoted early healing after ACLR.

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“…Exosomes are endosomal membrane-derived vesicles, approximately 50–150 nm in size, formed during endocytosis and secreted by almost all types of cells, and are present in large numbers in body fluids such as blood, urine, cerebrospinal fluid, tears, and saliva. Their main constituents are lipids, proteins, and nucleic acids, including microRNAs (miRNAs), messenger RNA (mRNA), and DNA derived from secretory cells transferred to other cells [ 12 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 , 75 , 76 , 77 , 78 , 79 , 80 , 81 , 82 , 83 , 84 , 85 , 86 , 87 , 88 , 89 , 90 , 91 , 92 , 93 , 94 , 95 , 96 , 97 , 98 , 99 , 100 , 101 , 102 , 103 , 104 , 105 , 106 ,…”

Section: Introductionmentioning

confidence: 99%

Regulation of Extracellular Vesicle-Mediated Immune Responses against Antigen-Specific Presentation

Matsuzaka

Yashiro

2022

Vaccines

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Extracellular vesicles (EVs) produced by various immune cells, including B and T cells, macrophages, dendritic cells (DCs), natural killer (NK) cells, and mast cells, mediate intercellular communication and have attracted much attention owing to the novel delivery system of molecules in vivo. DCs are among the most active exosome-secreting cells of the immune system. EVs produced by cancer cells contain cancer antigens; therefore, the development of vaccine therapy that does not require the identification of cancer antigens using cancer-cell-derived EVs may have significant clinical implications. In this review, we summarise the molecular mechanisms underlying EV-based immune responses and their therapeutic effects on tumour vaccination.

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Circulating extracellular vesicles and rheumatoid arthritis: a proteomic analysis

Cited by 32 publications

References 296 publications

Comprehensive overview of microRNA function in rheumatoid arthritis

Comprehensive overview of microRNA function in rheumatoid arthritis

BMSC-derived exosomes promote tendon-bone healing after anterior cruciate ligament reconstruction by regulating M1/M2 macrophage polarization in rats

Regulation of Extracellular Vesicle-Mediated Immune Responses against Antigen-Specific Presentation

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