Circulating Fibroblast Growth Factor-2 precipitates HIV-nephropathy in mice

Abstract

Over 80% of all children living with HIV reside in Africa and are at risk of developing HIV-associated nephropathy (HIVAN). Once HIVAN is established in children, it is difficult to revert its progression to chronic kidney failure even using antiretroviral drugs. Therefore, new therapeutic strategies are needed. Previous studies showed that the risk of developing HIVAN increases in children with high circulating levels of FGF-2, but it is unclear whether FGF-2 per se precipitates HIVAN. To unravel the role of circulating FGF-2 in childhood HIVAN, we used the HIV-Tg26 mouse model of HIVAN. Briefly, we demonstrated that circulating FGF-2 was preferentially recruited in the kidney of HIV-Tg26 mice with renal disease, and precipitated HIVAN in young mice without pre-existing kidney disease by activating the pERK pathway in renal epithelial cells without previously inducing the expression of HIV-1 genes. Wild type mice injected with recombinant adenoviral FGF-2 vectors (rAd-FGF-2) carrying a secreted form of human FGF-2 developed transient and reversible HIVAN-like lesions, including proteinuria and glomerular enlargement. HIV-Tg26 mice injected with rAd-FGF-2 developed more significant proliferative and pro-fibrotic inflammatory lesions, similar to those seen in childhood HIVAN. These lesions were partially reversed in mice treated with the FGF/VEGF receptor tyrosine kinase inhibitor PD173074. In conclusion, we developed a new FGF-2-inducible model of childhood HIVAN, and showed that high circulating levels of FGF-2 precipitated HIVAN without inducing the renal expression of HIV-genes. These findings suggest that high plasma FGF-2 levels may be an independent risk factor for precipitating HIVAN in young children.

New developments in the biology of fibroblast growth factors

“…High serum FGF2 levels were predictive of children at risk of developing HIV-associated nephropathy (HIVAN). In mice, FGF2 activated phospho-ERK in renal epithelial cells and induced transient and reversible HIVAN-like lesions, including proteinuria and glomerular enlargement (Das et al, 2021).…”

L-glutamine supplementation reduced morphological damage in the renal glomerulus of rats with Walker-256 tumor

“…Glomerular and tubular cells react to the inflammatory process with basement membrane lesion and with epithelial-mesenchymal transition, thereby converting the cells into fibroblasts with the ability to produce collagen, which in turn can cause damage to the renal vessels, tubules, and glomeruli, leading to renal fibrosis 35 , stimulated by the action of FGF-2 36 .…”