Circulating fibrocyte mobilization in negative pressure wound therapy

Abstract

Non-healing diabetic wounds are difficult to treat. They also create heavy financial burdens for both patients and society. Negative pressure wound therapy (NPWT) has been adopted to treat intractable wounds and has proved to be effective. However, the mechanisms that underlie the effects of this treatment are not entirely understood. Circulating fibrocytes are unique haematopoietic-derived stem cells that have been reported to play a pivotal role in wound healing. Here, we have investigated the effect of NPWT on fibrocyte mobilization and the role of fibrocyte mobilization in the healing of diabetic wounds during NPWT. We show that the NPWT group exhibited 2.6-fold to 12.1-fold greater numbers of tail vein-injected PKH-26-labelled fibrocytes in the diabetic wound sites compared with the control group. We also demonstrate that the fullthickness skin wounds treated with NPWT exhibit significantly reduced mRNA and protein expression, blood vessel density and proliferating cells when exogenous fibrocyte mobilization is inhibited. We speculate that systemic mobilization of fibrocytes during NPWT may be a mechanism for healing intractable wounds in a diabetic rat model experiment and that enhancement of cell mobilization may represent a potential treatment idea for intractable wound healing across all fields of surgery.

Tranexamic acid is associated with selective increase in inflammatory markers following total knee arthroplasty (TKA): a pilot study

“…IL-4, in combination with GM-CSF, can further promote inflammation by increasing differentiation of monocytes into dendritic cells [ 44 ]. Bellini and colleagues also showed that IL-4 can stimulate a unique circulating leukocyte subpopulation (0.1–0.5%) of bone marrow-derived stem cells known as fibrocytes that leave the blood and enter the site of healing and differentiate into fibroblasts/myofibroblasts with increased production of cell matrix components, growth factors, and inflammatory cytokines [ 45 – 47 ]. Therefore, in the current study, it is possible that IL-4 contributes to a heightened systemic inflammatory response observed in TXA patients (Figs 3 , 4 ).…”

Negative pressure wound therapy in the treatment of diabetic foot ulcers may be mediated through differential gene expression

“…One hypothesis has proposed that NPWT acts through alteration in the gene expression profile. This has been supported in animal models and preliminarily in humans [ 7 – 13 ], however, has never been systematically examined.…”

Fixierung von Hauttransplantaten mit Vakuumversiegelung: Eine sinnvolle Option?

“…Collagen production and cell migration are also induced [9]. The latest data show that NPWT leads to the accumulation of circulating fibrocytes in wounds as well [4]. This paper demonstrates that the NPWT can additionally induce a systemic effect.…”