Circulating Free Nucleic Acid Expression in Spinal Cord Injury

Abstract

Background: Spinal Cord Injury (SCI) remains one of the most devastating and difficult to manage medical pathologies despite the tremendous progress in neuroscience and neurosurgery. These injuries bear substantial personal and economic burden. SCI is the underlying cause for 1 of every 40 patients admitted to a major trauma centre in EGYPT. The prevalence rate of SCD was 63/100,000 for the total population. Traumatic spinal cord injury had a prevalence of 18/100,000, while non-traumatic SCI was found in 45/100,000 Aim of the work: we aimed to explore the role of circulating TP53INP2 mRNA in spinal cord injury pathogenesis. Patients and methods: We used bioinformatics analysis to identify autophagy related pathway and spinal cord-specific mRNA, real-time reverse-transcription PCR for determining the relative expression of TP53INP2 mRNA in sera and tissue samples. We assessed the serum expression of the chosen mRNA in 23 individuals with acute spinal cord injury, 41 individuals with chronic spinal cord injury, and 36 healthy control. Results: Our study revealed that TP53INP2 mRNA expression was significantly increased in the serum of SCI patients compared with that of the control group. Conclusion: The results demonstrated the significant high expression ofTP53INP2 mRNA in SCI patients, further findings arising from this study will help to guide therapeutic strategies for SCI.