Circulating FSH isoform patterns during recurrent increases in FSH throughout the oestrous cycle of heifers

Cooke, D. J.; Crowe, M.A.; Roche, J. F.

doi:10.1530/jrf.0.1100339

Cited by 67 publications

(43 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In contrast, Kojima et al [101] reported no change in FSH isoform distribution in the pituitary during the follicular phase of the estrous cycle in heifers. In agreement with this, Cooke et al [102] also reported no changes in circulatory FSH isoforms during the first or second follicular waves in beef heifers, however, did identify greater amounts of less acidic FSH isoforms during the preovulatory gonadotropin surge which was also associated with increased estradiol concentrations. In addition, Crowe et al [103] examined the resumption of follicular waves post partum and identified no significant differences in FSH heterogeneity between late pregnancy and the early post partum period.…”

Section: Factors Influencing Gonadotropin Isoform Profilesupporting

confidence: 57%

Structural and Functional Roles of FSH and LH as Glycoproteins Regulating Reproduction in Mammalian Species

Mullen¹,

Cooke²,

Crow³

2013

Gonadotropin

View full text Add to dashboard Cite

Section: Factors Influencing Gonadotropin Isoform Profilesupporting

confidence: 57%

Structural and Functional Roles of FSH and LH as Glycoproteins Regulating Reproduction in Mammalian Species

Mullen¹,

Cooke²,

Crow³

2013

Gonadotropin

View full text Add to dashboard Cite

“…Thus, in addition to critical LH pulses, the signal that specifically increases granulosa cell aromatase activity may also have been lacking in anovulatory cows. It has previously been demonstrated that a shift in the FSH isoform distribution occurs prior to ovulation in human beings (13,28) and cattle (10). Though less acidic isoforms have a short half-life in circulation (12), they have potent biological activities at the level of the ovary (11,29), and display a greater 205 capacity to induce aromatase expression than the more acidic isoforms (25).…”

Section: A Concentrations 195mentioning

confidence: 99%

“…The isoelectric point (pI) of FSH has a wide range depending on the abundance of sialic acid residues; heavily sialylated isoforms have an acid pH, and isoforms with little or no sialic acid have a less acidic or basic pH. In 40 cattle, most (~70%) FSH isoforms have a pI less than 5.0 (10). The heterogeneity in glycosylation has significant effects on the biological properties of FSH.…”

Section: Introduction 20mentioning

confidence: 99%

Follicle-stimulating hormone isoforms and plasma concentrations of estradiol and inhibin A in dairy cows with ovulatory and non-ovulatory follicles during the first postpartum follicle wave

Butler

Pelton

Knight

et al. 2008

Domestic Animal Endocrinology

View full text Add to dashboard Cite

Following parturition, all cows display a wave of ovarian follicular growth, but a large proportion fail to generate a preovulatory rise in estradiol, and hence fail to ovulate. Follicle stimulating hormone (FSH) exists as multiple isoforms in the circulation depending on the type and extent of glycosylation, and this has pronounced effects on its biological properties. This 5 study examined differences in plasma FSH, estradiol, and inhibin A concentrations, and the distribution of FSH isoforms in cows with ovulatory or atretic dominant follicles during the first postpartum follicle wave. Plasma FSH isoform distribution was examined in both groups during the period of final development of the dominant follicle by liquid phase isoelectric focusing.Cows with an ovulatory follicle had higher circulating estradiol and inhibin A concentrations, 10 and lower plasma FSH concentrations. The distribution of FSH isoforms displayed a marked shift toward the less acidic isoforms in cows with ovulatory follicles. A higher proportion of the FSH isoforms had a pI >5.0 in cows with ovulatory follicles compared to those with atretic follicles. In addition, cows with ovulatory follicles had greater dry matter intake, superior energy balance, elevated circulating concentrations of insulin and insulin-like growth factor-I, 15 and lower plasma nonesterified fatty acids. The shift in FSH isoforms toward a greater abundance of the less acidic isoforms appears to be a key component in determining the capability for producing a preovulatory rise in estradiol, and this shift in FSH isoforms was associated with more favorable bioenergetic and metabolic status.

show abstract

“…El polimorfismo de las gonadotropinas se ha documentado en rumiantes (6,7,8) y mediante el cromatoenfoque se han identificado isoformas de la LH en el suero (9,10) como en la hipófisis (11,12) y cuya proporción relativa es diferente (6) . Incluso se sabe que la proporción de estas isoformas en la circulación es distinta en diversas etapas del ciclo estral (9,10) .…”

Section: Introductionunclassified

“…Polymorphism in gonadotropins occurs in ruminants (6,7,8) . Using the chromatofocusing protein separation technique, different proportions of LH isoforms have been identified in the serum (9,10) and hypophysis (11,12) .…”

Section: Introductionmentioning

confidence: 99%

Biological and immunological activity in bovine luteinizing hormone charge isoforms

Ortega¹,

Olivares

Murcia³

et al. 2016

Rev. Mex. Cienc. Pecu.

View full text Add to dashboard Cite

RESUMENLa hormona luteinizante (LH) sufre modificaciones postraduccionales que dan origen a isoformas de carga. El estudio determinó la actividad biológica (B) e inmunológica (I) de distintas isoformas de LH bovina. Las isoformas se aislaron mediante el cromatoenfoque a partir del extracto glicoprotéico obtenido de lóbulos anteriores de hipófisis de bovino. La actividad biológica se evaluó en un bioensayo in vitro midiendo la producción de AMPc. La actividad inmunológica se midió con un radioinmunoensayo (RIA) específico para LH. El USDA-bLH-B5 se utilizó como referencia. Las isoformas se agruparon tomando como referencia el rango de pH de elución, en básicas (A, pH, 10.75-9.75; B, pH, 9.58-8.41), neutras (C, pH, 7.98-6.89) y ácidas (D, pH, 6.88-5.41; E, pH, 5.36-3.46). El peso molecular del heterodímero de cada isoforma y del estándar fue similar, estimado en 36.5 kDa. La actividad inmunológica y biológica se comportó de forma dosis-dependiente. Con respecto al estándar, se requirió una mayor concentración de proteína de cada isoforma para obtener el IC50 en la curva de inhibición. En el bioensayo, el valor EC50 para la producción de AMPc fue significativamente diferente entre isoformas; la isoforma neutra mostró un EC50 inferior lo que se interpretó como la proteína más bioactiva, en contraste, la isoforma ácida, mostró un valor de EC50 superior y resultó ser la menos bioactiva; la básica tuvo un comportamiento intermedio (P<0.05). En conclusión, los resultados sugieren un efecto diferenciado de las isoformas de carga, sobre la producción cuantitativa de AMPc por unidad de LH inmunoreactiva. PALABRAS CLAVE: Hormona luteinizante, Actividad biológica, Actividad inmunológica. ABSTRACT Luteinizing hormone (LH) undergoes posttranslational modifications that originate different charge isoforms. The study evaluated the differences in biological (B) and immunological (I) activity between isoforms of bovine LH.Isoforms were isolated by chromatofocusing from anterior pituitary glycoprotein extract. The biological activity was evaluated in an in vitro bioassay. Immunological activity was measured with a radioimmunoassay (RIA) specific for LH. The USDA-bLH-B5 standard was utilized as the reference. LH isoforms were grouped by their pH range of elution, in basic (A, pH, 10.75-9.75; B, pH 9.58-8.41), neutral (C pH, 7.98-6.89) and acidic (D, pH, 6.88-5.41; E, pH 5.36-3.46). The molecular weight of the heterodimer of each isoform was similar to the LH standard, estimated to be 36.5 kDa. Immunological and biological activity behaved in a dose-dependent manner. With respect to the LH standard, all isoforms required higher protein concentration to reach the IC50 of the inhibition curve. In the bioassay EC50 value for cAMP production was significantly different among isoforms; the neutral isoform showed a lower EC50 which was interpreted as more bioactive, the acidic isoform E showed an EC50 being the least bioactive and the basic was intermediate (P<0.05). In conclusion, the results suggest a quantitative effect of LH charge iso...

show abstract

Circulating FSH isoform patterns during recurrent increases in FSH throughout the oestrous cycle of heifers

Cited by 67 publications

References 10 publications

Structural and Functional Roles of FSH and LH as Glycoproteins Regulating Reproduction in Mammalian Species

Structural and Functional Roles of FSH and LH as Glycoproteins Regulating Reproduction in Mammalian Species

Follicle-stimulating hormone isoforms and plasma concentrations of estradiol and inhibin A in dairy cows with ovulatory and non-ovulatory follicles during the first postpartum follicle wave

Biological and immunological activity in bovine luteinizing hormone charge isoforms

Contact Info

Product

Resources

About