Circulating IL-10 is compromised in patients predisposed to developing and in patients with severe knee osteoarthritis

Abstract

The purpose of this investigation was to identify if serum interleukin (IL)-10 and tumor necrosis factor (TNF)-α concentrations and their ratio (IL-10/TNF-α) are altered in subjects predisposed to developing knee osteoarthritis following ligamentous injury and in those with severe knee osteoarthritis. Serum IL-10 and TNF-α concentrations were measured in four groups of subjects (n = 218): (1) reportedly-healthy and non-injured control subjects (CON; n = 92), (2) subjects scheduled to undergo anterior cruciate ligament surgery (ACL; n = 42), (3) non-surgical subjects with knee osteoarthritis (OA; n = 60), and (4) subjects with knee osteoarthritis scheduled to undergo total knee arthroplasty (TKA; n = 24). X-ray images were used to grade the severity of knee osteoarthritis. Serum IL-10 and the serum IL-10/TNF-α ratio were significantly lower while serum TNF-α was not significantly perturbed with severe compared to moderate knee osteoarthritis (i.e., Kellgren-Lawrence grade 4 vs. 3, respectively). Serum IL-10 was significantly lower in the absence of serum TNF-α alterations in the ACL group. We conclude that serum IL-10 concentrations are compromised in subjects predisposed to developing knee osteoarthritis following ligamentous trauma and in subjects with radiographic evidence of severe knee osteoarthritis.

Small Extracellular Vesicles from Inflamed Adipose Derived Stromal Cells Enhance the NF-κB-Dependent Inflammatory/Catabolic Environment of Osteoarthritis

“…Synoviocytes showed a similar trend: at 4 h, the presence of sEVs_IL-1 induced a higher expression of cytokines/chemokines, extracellular matrix degradative enzymes, and inflammatory/pain genes (IL-6, -8, MCP-1, IL-1 β , TNF- α , MMP-1, -13, ADAMTS-4, COX-2, and VEGF,), compared to the CTR (Figures 4 and 5 and Supplementary Figure 5 ). As with the chondrocytes, in comparison with the control cells, at 15 h, sEVs_IL-1 did not further increase the expression of these genes [ 36 ].…”

Individual immune cell and cytokine profiles determine platelet-rich plasma composition

“…This is highly relevant, since high systemic levels of distinct T cell subsets have been associated with impaired tissue healing [ 23 , 49 ]. These TEMRA T cells accumulate in large numbers also at the injury site and are the major local producers of pro-inflammatory cytokines [ 23 ], which are, again, linked to the age-related phenotype [ 50 ] and the development of OA [ 51 ]. Furthermore, a local downregulation of these cells led to a decreased concentration of pro-inflammatory cytokines and an improved bone regeneration in a preclinical fracture model [ 52 ].…”

Vitamin D Supplementation Has No Impact on Cardiorespiratory Fitness, but Improves Inflammatory Status in Vitamin D Deficient Young Men Engaged in Resistance Training

“…Our second hypothesis that Vit-D supplementation would potentiate the anti-inflammatory effect of RT in Vit-D-deficient young healthy men is supported by the increased IL-10/TNF-α ratio observed in the VD group over the 12-week supplementation and RT period, while there was no such change in the PLC group. IL-10 and TNF-α are considered as a quintessential anti-inflammatory cytokine and prototypical pro-inflammatory cytokine, respectively [72]. As IL-10 reciprocally down-regulates pro-inflammatory cytokine production [72], serum IL-10/TNF-α ratio has been used as an anti-inflammatory index in many previous studies [41,[73][74][75].…”