Circulating interferon production in pigs infected with hog cholera virus

Torlone, V.; Titoli, F.; Gialletti, L.

doi:10.1016/0024-3205(65)90218-3

Cited by 14 publications

(2 citation statements)

References 10 publications

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“…This expression pattern contradicts the host response observed for other viruses, such as African swine fever virus (CSFV) [33], classical swine fever virus [34] and influenza A virus [35], and that was observed in follicles and PAMs of PMWS piglets [36,37]. Increased expression of MHC class II molecular have been shown to be correlated to increased interferon production in pigs infection infected with hog cholera virus [38]. IFN-γ up-regulation have been shown after 48 HPI, its up-regulation could benefit PCV2 replication [39].…”

Section: Mhc Class II Moleculesmentioning

confidence: 80%

Transcription analysis of the porcine alveolar macrophage response to porcine circovirus type 2

Liu

Wang

et al. 2013

BMC Genomics

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BackgroundPorcine circovirus type 2 (PCV2) is the causal agent of postweaning multisystemic wasting syndrome (PMWS), which has severely impacted the swine industry worldwide. PCV2 triggers a weak and atypical innate immune response, but the key genes and mechanisms by which the virus interferes with host innate immunity have not yet been elucidated. In this study, genes that control the response of primary porcine alveolar macrophages (PAMs), the main target of PCV2, were profiled in vitro.ResultsPAMs were successfully infected by PCV2-WH strain, as evidenced quantitative real-time polymerase chain reaction (qPCR) and immunofluorescence assay (IFA) results. Infection-related differential gene expression was investigated using pig microarrays from the US Pig Genome Coordination Program and validated by real-time PCR and enzyme-linked immunosorbent assay (ELISA). Microarray analysis at 24 and 48 hours post-infection (HPI) revealed 266 and 175 unique genes, respectively, that were differentially expressed (false discovery rate <0.05; fold-change >2). Only six genes were differentially expressed between 24 and 48 HPI. The up-regulated genes were principally related to immune response, cytokine activity, locomotion, regulation of cell proliferation, apoptosis, cell growth arrest, and antigen procession and presentation. The down-regulated genes were mainly involved in terpenoid biosynthesis, carbohydrate metabolism, translation, proteasome degradation, signal transducer activity, and ribosomal proteins, which were representative of the reduced vital activity of PCV2-infected cells.ConclusionsPCV2 infection of PAMs causes up-regulation of genes related to inflammation, indicating that PCV2 may induce systematic inflammation. PCV2 persistently induced cytokines, mainly through the Toll-like receptor (TLR) 1 and TLR9 pathways, which may promote high levels of cytokine secretion. PCV2 may prevent apoptosis in PAMs by up-regulating SERPINB9 expression, possibly to lengthen the duration of PCV2 replication-permissive conditions. The observed gene expression profile may provide insights into the underlying immunological response and pathological changes that occur in pigs following PCV2 infection.

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Section: Mhc Class II Moleculesmentioning

confidence: 80%

Transcription analysis of the porcine alveolar macrophage response to porcine circovirus type 2

Liu

Wang

et al. 2013

BMC Genomics

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“…The increase in SLA class I and II expression at 4 DPI may be due to the release of soluble factors such as interferon gamma, which has been described as a potent stimulator of MHC class I and class II antigen expression [22]. This view is supported by the fact that spleen sections from pigs infected with hog cholera, a virus which is known to stimulate high interferon production [23], had higher expression of SLA than controls. Whether the increase in SLA expression seen in spleens from ASFV DR-II infected pigs is due to gamma interferon or to other soluble factors remains to be determined.…”

Section: Discussionmentioning

confidence: 68%

Modulation of splenic macrophages, and swine leukocyte antigen (SLA) and viral antigen expression following African swine fever virus (ASFV) inoculation

González-Juarrero

Lunney²,

Sánchez-Vizcaíno

et al. 1992

Archives of Virology

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Expression of viral and major histocompatibility complex (MHC) antigens and localization of T cells and macrophages was studied in frozen tissue sections of spleens taken from normal pigs or from pigs inoculated with highly virulent Lisbon 60 (L60), or with moderately virulent Dominican Republic 1978 (DR-II), African swine fever virus (ASFV) isolates. Splenic sections from L60 inoculated pigs exhibited a large decrease in macrophage staining, whereas DR-II infected animals appeared more intensely stained in the macrophage sheath arteries. Class I and class II MHC expression was decreased in spleens from pigs infected with either isolate at 3 day post inoculation (DPI). This was reversed in DR-II inoculated pigs at 4 DPI. Splenic tissue sections from L60 inoculated pigs exhibited only a marginal increase in SLA expression at a later time, 6 DPI. We suggest that the recovery of SLA expression during infection of pigs with ASFV is associated with survival or replacement of macrophages in the spleen leading to an effective immune response against the virus.

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Purification of Interferons

Fantes¹

1968

Ciba Foundation Symposium ‐ Interferon

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Circulating interferon production in pigs infected with hog cholera virus

Cited by 14 publications

References 10 publications

Transcription analysis of the porcine alveolar macrophage response to porcine circovirus type 2

Transcription analysis of the porcine alveolar macrophage response to porcine circovirus type 2

Modulation of splenic macrophages, and swine leukocyte antigen (SLA) and viral antigen expression following African swine fever virus (ASFV) inoculation

Purification of Interferons

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