Circulating Long Noncoding RNA UCA1 as a Novel Biomarker of Acute Myocardial Infarction

Yan, Youyou; Zhang, Bin; Liu, Ning; Qi, Chao; Yang, Xiao; Tian, Xin; Li, Tianyi; Liu, Bin

doi:10.1155/2016/8079372

Cited by 108 publications

(98 citation statements)

References 26 publications

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“…LncRNA discrimination antagonising non-protein coding RNA (DANCR) encodes human chromosome 4q12 and it was firstly identified as epidermal cell differentiation suppressor [12], but further study revealed that it acted as an oncogene in various kinds of cancers, such as colon cancer [13], prostate cancer [14] and hepatocellular carcinoma [15]. However, the effect of DANCR in cardiovascular diseases has never been explored.…”

Section: Introductionmentioning

confidence: 99%

Long non-coding RNA DANCR alleviates hypoxia-caused H9c2 cells damage through up regulation of HIF-1α

Qiu

Zhao

Dai

et al. 2020

Artificial Cells, Nanomedicine, and Biotechnology

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Hypoxia is an important cause of myocardial cell loss, further inducing various heart illnesses, including acute myocardial infraction (AMI). Long non-coding RNA (LncRNA) discrimination antagonising non-protein coding RNA (DANCR) was firstly identified as epidermal cell differentiation suppressor. Here, we aimed to explore the effects and mechanism of DNACR in hypoxia-induced H9c2 cells. Hypoxic cells were made through 94% N 2 , 5% CO 2 , and 1% O 2 environment for 24 h. Cell viability and apoptosis were detected via methyl thiazolyl tetrazolium (MTT) method and flow cytometry analysis, respectively. The expression of DANCR and HIF-1a was examined via qRT-PCR. The expression of proteins related to cell apoptosis and PI3K/AKT/mTOR and ERK1/2 signal pathways was examined through western blot analysis. We found that hypoxia induced obvious cell activity inhibition and apoptosis increasing in H9c2 cells. DANCR was negatively regulated under hypoxia condition. Overexpression of DANCR rescued activity and attenuated apoptosis. Moreover, the overexpression of DANCR elevated the activation of PI3K/AKT/mTOR and ERK1/2 pathways. Further study indicated that DANCR could up-regulate the expression of HIF-1a. Si-HIF-1a transfection could remove the beneficial effects of DANCR overexpression in hypoxia-caused H9c2 cells damage. In conclusion, DANCR alleviated hypoxia-caused H9c2 cells damage through positive regulation of HIF-1a.GRAPHICAL ABSTRACT ARTICLE HISTORY

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Section: Introductionmentioning

confidence: 99%

Long non-coding RNA DANCR alleviates hypoxia-caused H9c2 cells damage through up regulation of HIF-1α

Qiu

Zhao

Dai

et al. 2020

Artificial Cells, Nanomedicine, and Biotechnology

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“…Based on this, lncRNAs have been previously investigated for their potential role as cancer biomarkers in body fluids (either in plasma, serum or urine) [13][14][15][16][17][18]. For example, the lnc-RNA UCA1 (urothelial carcinoma associated 1) is a lncRNA revealed as a potential biomarker for bladder cancer [19], Also in prostate cancer patients, fragments from different regions of MALAT1 transcript were detected in plasma at higher copy number than in non-prostate cancer patients [20].…”

Section: Introductionmentioning

confidence: 99%

Three Circulating LncRNA Predict Early Progress of Esophageal Squamous Cell Carcinoma

Zheng

2016

Cell Physiol Biochem

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Background/Aims: Previous studies revealed that circulating (either from plasma or serum) long non-coding RNA may predict the occurrence or prognosis of multiple human malignant tumors. In this study, we mainly explored whether circulating lncRNAs can be utilized as biomarkers predicting the development of human esophageal squamous cell carcinoma (ESCC). Methods: LncRNA microarray was applied to screen the potential biomarkers for ESCC. Each group contained three individual plasma samples. A multi-stage validation and risk score formula detection were used for validation. Results: Eleven dysregulated lncRNAs were obtained after Venny analysis. Further validation in a larger cohort including 205 ESCC patients, 82 patients suffering from esophagus dysplasia and 210 healthy controls confirmed that increased Linc00152, CFLAR-AS1 and POU3F3 might be potential biomarkers for predicting the early progress with an area under curve (AUC) of 0.698, 0.651 and 0.584, respectively. The merged AUC of the three factors and merged with CEA was 0.765 and 0.955, respectively. We also revealed that circulating levels of three lncRNAs were associated with poor post-surgery prognosis of ESCC patients. Conclusions: The three circulating lncRNAs might serve as potential biomarkers for predicting the early occurrence of ESCC.

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“…The plasma concentration of the lncRNA CoroMarker was recently recognized as a novel biomarker for the diagnosis of coronary artery disease due to the stability, sensitivity and specific properties of CoroMarker in these patients [30]. The circulating concentration of the lncRNA UCA1 has also been identified as a potential novel biomarker for the prognosis of AMI [31]. These observations support the potential for lncRNAs to be used as biomarkers and therapeutics in ischaemic heart injury, especially AMI.…”

Section: Discussionmentioning

confidence: 83%

Circulating Long Noncoding RNA HOTAIR is an Essential Mediator of Acute Myocardial Infarction

Gao¹,

Liu²,

Guo³

et al. 2017

Cell Physiol Biochem

124

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Background/Aims: Acute myocardial infarction (AMI) is one of the leading causes of death in the world. However, specific diagnostic biomarkers have not been fully determined, and candidate regulatory targets for AMI have not been identified to date. Long noncoding RNAs (lncRNAs) are a class of RNA molecules that have diverse regulatory functions during embryonic development, normal life, and disease in higher organisms. However, research on the role of lncRNAs in cardiovascular diseases, particularly AMI, is still in its infancy. HOX antisense intergenic RNA (HOTAIR), a 2.2 kb lncRNA, was initially described as a modulator of HOX gene expression. Recent studies have illustrated the important role of HOTAIR in cancer progression, but few studies have reported its function in cardiac disease, including AMI. In the current study, we aimed to detect the expression of HOTAIR during AMI and to explore its function in hypoxia-induced cardiomyocyte injury in neonatal cardiomyocytes. Methods: In 50 consecutively enrolled AMI patients, we examined the serum expression levels of HOTAIR and analysed its correlation with cardiac troponin I (cTnI) expression. Another 50 age- and sex-matched subjects served as healthy controls. Next, the HOTAIR expression was detected in the serum from C57BL/6J mice subjected to coronary artery ligation and in neonatal rat cardiomyocytes induced by hypoxia. Cultured cardiomyocytes apoptosis were measured by terminal deoxynucleotide transferase dUTP nick end labelling (TUNEL) staining. A search for miRNAs that had complementary base paring with HOTAIR was performed utilizing an online software program, and the interaction between miR-1 and HOTAIR was examined using a luciferase reporter assay. Results: Our study revealed that HOTAIR expression was significantly decreased in the serum of AMI patients compared with that of the healthy controls. Similarly, we observed that HOTAIR was downregulated in the serum of mice subjected to coronary artery ligation and in cultured cardiomyocytes exposed to hypoxia. Furthermore, we observed that the adenovirus vector-driven overexpression of HOTAIR dramatically limited hypoxia-induced myocyte apoptosis, whereas knockdown HOTAIR by AdshHOTAIR (adenoviral short hairpin HOTAIR) exhibited the opposite phenotype. Mechanistically, we discovered that the cardioprotective function of HOTAIR is partly based on the negative regulation of miR-1. Conclusions: Taken together, the results of our study suggest that HOTAIR is a protective factor for cardiomyocytes and that the plasma concentration of HOTAIR may serve as a biomarker for human AMI diagnosis.

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Circulating Long Noncoding RNA UCA1 as a Novel Biomarker of Acute Myocardial Infarction

Cited by 108 publications

References 26 publications

Long non-coding RNA DANCR alleviates hypoxia-caused H9c2 cells damage through up regulation of HIF-1α

Long non-coding RNA DANCR alleviates hypoxia-caused H9c2 cells damage through up regulation of HIF-1α

Three Circulating LncRNA Predict Early Progress of Esophageal Squamous Cell Carcinoma

Circulating Long Noncoding RNA HOTAIR is an Essential Mediator of Acute Myocardial Infarction

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