Circulating Microparticles and Procoagulant Activity in Elderly Patients

Forest, A; Pautas, Éric; Ray, Patrick; Bonnet, Dominique; Verny, Marc; Amabile, Nicolas; Boulanger, Chantal M.; Riou, Bruno; Tedgui, Alain; Mallat, Ziad; Boddaert, Jacques

doi:10.1093/gerona/glp187

Cited by 44 publications

(35 citation statements)

References 44 publications

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“…Interestingly, angiotensin II may up-regulate the generation of pro-coagulant MPs by human mononuclear cells that confirms a hypothesis about linking between the renin-angiotensin systems to thrombosis [45]. Therefore, there is evidence that elderly persons compared with young persons may have different patterns of expression of mononuclear cells derived MPs and pro-coagulant activity in stable condition and diseases [46].…”

Section: Biological Role and Function Of Mpssupporting

confidence: 59%

The Clinical Utility of Circulating Microparticles’ Measurement in Heart Failure Patients

Berezin¹

2016

Vasc Med Surg

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Heart failure (HF) continues to have a sufficient impact on morbidity, mortality and disability in developed countries. Growing evidence supports the hypothesis that microparticles (MPs) might contribute to the pathogenesis of the HF development paling a pivotal role in the regulation of the endogenous repair system, thrombosis, coagulation, inflammation, immunity and metabolic memory phenomenon. Therefore, there is a large body of data clarifying the predictive value of MP numerous in circulation among subjects with HF. Although determination of MP signature is better than measurement of single MP circulating level, there is not yet closely confirmation that immune phenotype of cells produced MPs are important for HF prediction and development. The aim of the review: to summarize knowledge regarding the measurement of number of various MPs subsets in HF patients.

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Section: Biological Role and Function Of Mpssupporting

confidence: 59%

The Clinical Utility of Circulating Microparticles’ Measurement in Heart Failure Patients

Berezin¹

2016

Vasc Med Surg

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“…31 Conversely, Forest et al reported that endothelial MPs decrease in individuals aged 75 years and older. 32 Each of these studies involved in vivo measurement of plasma MP levels, where MP clearance may be a significant determinant of concentration. As aging is known to impair clearance of apoptotic cells, 33 we examined MP formation in vitro using a model of endothelial senescence through long-term culture as previously detailed.…”

Section: Discussionmentioning

confidence: 99%

Microparticles Induce Cell Cycle Arrest Through Redox‐Sensitive Processes in Endothelial Cells: Implications in Vascular Senescence

Burger

Kwart

Montezano

et al. 2012

JAHA

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BackgroundChronic disease accelerates endothelial dysfunction in aging, a process associated with cell senescence. However, the mechanisms underlying this process are unclear. We examined whether endothelial cell (EC)-derived microparticles (MPs) facilitate EC senescence and questioned the role of reactive oxygen species in this process.Methods and ResultsSenescence was induced by sequential passaging of primary mouse ECs. Cells retained phenotypic characteristics of ECs from passage 4 through passage 21. Passage 21 ECs exhibited features of senescence, including increased staining of senescence-associated β-galactosidase (SA-βgal), a greater percentage of cells in G1/G0 phase of the cell cycle, and increased phosphorylation of p66Shc (P<0.05). Microparticle formation from passage 21 ECs was increased versus passage 4 ECs (∼2.2-fold increase versus passage 4, P<0.05), and the Rho kinase inhibitor fasudil blocked this increase. Exposure of passage 4 ECs to MPs shifted cells from a proliferating to a nonproliferating phenotype, as indicated by cell cycle analysis and increased senescence-associated β-galactosidase staining. MPs increased EC generation of O2•− (∼2.7-fold) and H2O2 (∼2.6-fold), effects blocked by apocynin (nicotinamide adenine dinucleotide phosphate oxidase inhibitor) and rotenone (mitochondrial oxidase inhibitor) but not by allopurinol (xanthine oxidase inhibitor). MPs increased expression of cell cycle proteins p 21 cip1 and p16ink4a and stimulated phosphorylation of p66Shc in ECs (P<0.05 versus untreated ECs). Pretreatment with the reactive oxygen species scavenger sodium 4,5-dihydroxybenzene-1,3-disulfonate (tiron) abrogated the prosenescent effects of MPs.ConclusionsMPs promote EC senescence through nicotinamide adenine dinucleotide phosphate oxidase- and mitochondrial-derived reactive oxygen species. Such redox-sensitive processes may be important in vascular dysfunction in aging. (J Am Heart Assoc. 2012;1:e001842 doi: 10.1161/JAHA.112.001842.)

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“…The present results contrast, however, with those of Forest et al . [15] The latter authors reported healthy elderly subjects to produce fewer endothelial MVs. It may be that minor, undetected illnesses, influence the production of MVs.…”

Section: Discussionmentioning

confidence: 99%

Microvesicles from the plasma of elderly subjects and from senescent endothelial cells promote vascular calcification

Alique

Ruíz-Torres

Bodega

et al. 2017

Aging

106

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Vascular calcification is commonly seen in elderly people, though it can also appear in middle-aged subjects affected by premature vascular aging. The aim of this work is to test the involvement of microvesicles (MVs) produced by senescent endothelial cells (EC) and from plasma of elderly people in vascular calcification. The present work shows that MVs produced by senescent cultured ECs, plus those found in the plasma of elderly subjects, promote calcification in vascular smooth muscle cells. Only MVs from senescent ECs, and from elderly subjects' plasma, induced calcification. This ability correlated with these types of MVs' carriage of: a) increased quantities of annexins (which might act as nucleation sites for calcification), b) increased quantities of bone-morphogenic protein, and c) larger Ca contents. The MVs of senescent, cultured ECs, and those present in the plasma of elderly subjects, promote vascular calcification. The present results provide mechanistic insights into the observed increase in vascular calcification-related diseases in the elderly, and in younger patients with premature vascular aging, paving the way towards novel therapeutic strategies.

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Circulating Microparticles and Procoagulant Activity in Elderly Patients

Abstract: Elderly patients expressed a different pattern of MP in stable conditions, with a different response to sepsis in procoagulant activity modification.

Cited by 44 publications

References 44 publications

The Clinical Utility of Circulating Microparticles’ Measurement in Heart Failure Patients

The Clinical Utility of Circulating Microparticles’ Measurement in Heart Failure Patients

Microparticles Induce Cell Cycle Arrest Through Redox‐Sensitive Processes in Endothelial Cells: Implications in Vascular Senescence

Microvesicles from the plasma of elderly subjects and from senescent endothelial cells promote vascular calcification

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