Circulating MicroRNAs and the Occurrence of Acute Myocardial Infarction in Chinese Populations

Abstract

A cute myocardial infarction (AMI) is a leading cause of morbidity and mortality worldwide. 1 Multiple conventional risk factors and serum atherosclerotic biomarkers have been established for coronary heart disease (CHD). 2 However, advances in genomics and proteomics, especially gene-expression profiling using microarray and quantitative real-time polymerase chain reaction (qPCR), have promoted the generation of many novel molecular biomarkers for AMI with potential clinical values. 3,4 Recently, microRNAs (miRNAs) have attracted extensive interest in the field of cardiovascular diseases. 5 Clinical Perspective on p 198MiRNAs are short, endogenous, noncoding RNAs that regulate gene expressions at the posttranscriptional level by binding to the 3′-untranslated regions of their target mRNAs. 6,7 MiRNAs have now emerged as key regulators of cardiac growth, vascular development, and angiogenesis. 8,9 Recent studies demonstrated that miRNAs can be detected in circulating blood and may be useful as disease biomarkers. 10,11 The levels and identities of circulating miRNAs in cardiovascular diseases have been evaluated in a series of studies. For instance, levels of some vascular and inflammation-associated miRNAs were found to be significantly lower in the plasma of 67 patients with CHD when compared with controls, 12 and the cardiac-specific miRNA, miR-208a, was only detectable in 33 patients with AMI and not Background-Circulating microRNAs ( miRNAs) are emerging as novel disease biomarkers. We aimed to explore the association between circulating miRNAs and the occurrence of acute myocardial infarction (AMI) in Chinese populations. Methods and Results-In the discovery stage, the plasma of 20 patients with AMI and 20 controls were pooled respectively and profiled by massively parallel sequencing. Seventy-seven miRNAs showed differential expression. Selected miRNAs were validated in 178 patients with AMI and 198 controls using quantitative reverse transcriptase polymerase chain reaction assays and further replicated in 150 patients with AMI and 150 controls. Results suggest that miR-320b and miR-125b levels were significantly lower in patients with AMI than in controls in both validation populations (P<0.0001). Lower levels of miR-320b and miR-125b were associated with increased occurrence of AMI (adjusted odds ratio, 4.71; 95% confidence interval, 2.96-7.48 and odds ratio, 4.27; 95% confidence interval, 2.84-6.41, respectively). Addition of the 2 miRNAs to traditional risk factors led to a significant improvement in the area under the curve from 0.822 (95% confidence interval, 0.787-0.856) to 0.871 (95% confidence interval, 0.842-0.900), with a net reclassification improvement of 20.45% (P<0.0001) and an integrated discrimination improvement of 0.16 (P<0.0001) for patients with AMI. A functional study showed that miR-320b and miR-125b could regulate the expression profiles of genes enriched in several signal transduction pathways critical for coronary heart disease in human vascular endothelial cells. Conclusions-The...

Effect of microRNA-208a on mitochondrial apoptosis of cardiomyocytes of neonatal rats

“…According to previous researches, miRNA was involved in the physiological process of apoptosis, electrical conduction change, cardiomyocytes regeneration and cardiac remodeling during the myocardial ischemia and anoxia [14][15][16][17]. Besides, several researches have explained the mechanism of many miRNAs in the acute myocardial ischemia, which provide the reference for the treatment of heart diseases through the molecular drugs.…”

Circulating MiR-19b-3p, MiR-134-5p and MiR-186-5p are Promising Novel Biomarkers for Early Diagnosis of Acute Myocardial Infarction

“…Then, the RNA pellet was purged with 1 mL ethanol (75% v/v) to remove residual salt. For normalization, each sample was supplemented with 25 pM Caenorhabditi selegans miR-39 (cel-miR-39) after the addition of TRIzol, as previously reported [35].…”

Polycyclic Aromatic Hydrocarbons–Associated MicroRNAs and Heart Rate Variability in Coke Oven Workers

“…39 When we prepared for this study, we also demonstrated in another study that plasma level of miR-320b was significantly lower in acute myocardial infarction patients compared with control subjects. 40 MiR-27a-3p was involved in endothelial cell pathophysiology, 41 and miR-142-5p expression was decreased in the peripheral blood mononuclear cells of chronic heart failure patients. 42 Moreover, the predicted target genes of miR-142-5p overlapped much with the known chronic heart failure-modulated genes.…”