Circulating microRNAs Are Associated With Metabolic Markers in Adolescents With Hepatosteatosis

Abstract

BackgroundAltered hepatic microRNA (miRNA) expression may play a role in the development of insulin resistance (IR) and non-alcoholic fatty liver disease (NAFLD). Circulating miRNAs could mirror the liver metabolism.ObjectiveThis study aimed to assess the relationship between serum miRNA profile in children with obesity, IR, and NAFLD.MethodsAdolescents with obesity (n = 31) were stratified based on insulin resistance and NAFLD status. One-hundred seventy-nine miRNAs were determined in the serum by quantitative RT-PCR. Differentially expressed miRNAs were compared between groups, and log-transformed levels correlated with metabolic markers and intrahepatic triglyceride.ResultsSerum miR-21-5p, -22-3p, -150-5p, and -155-5p levels were higher in children with IR and NAFLD, and their expression levels correlated with hepatic fat and serum triglyceride. In patients with NAFLD, miR-155-5p correlated with ALT (r = 0.68, p<0.01) and AST (r = 0.64, p<0.01) and miR-21-5p and -22-3p levels correlated with plasma adiponectin (r = -0.71 and r = -0.75, respectively, p<0.05) and fibroblast growth factor-21 (r = -0.73 and r = -0.89, respectively, p<0.01). miR-27-3a level was higher in children without IR and NAFLD.ConclusionsSeveral miRNAs are differentially expressed in children with IR and NAFLD. Determining their mechanistic roles may provide newer diagnostic tools and therapeutic targets for pediatric NAFLD.

Diagnostic Significance of hsa-miR-21-5p, hsa-miR-192-5p, hsa-miR-155-5p, hsa-miR-199a-5p Panel and Ratios in Hepatocellular Carcinoma on Top of Liver Cirrhosis in HCV-Infected Patients

“…Correlation studies in all patients (n = 79) revealed that plasma hsa-miR-21-5p, expression level was positively correlated with serum insulin and the presence of insulin resistance and dyslipidemia (increased TC and TAG). Lin et al [ 34 ] suggested that altered plasma miRs might reflect liver lipid metabolism and said that hepatic miR expression contributes to the development of insulin resistance. This response is per both hsa-miR-21-5p and hsa-miR-155-5p would control the expression of genes involved in hepatic TAG and cholesterol metabolism, evidenced by silencing hepatic miR21 genes, where reduced hepatic inflammation and enhanced fibrosis were achieved [ 35 , 36 ].…”

Exploration of Shared Gene Signatures and Molecular Mechanisms Between Periodontitis and Nonalcoholic Fatty Liver Disease

“…We speculated that periodontitis might be affected by miR-125b-5p. Similarly, miR-17-5p and miR-21-5p were reported to play a part in periodontitis and migration of DCs and were predicted to get involved in NAFLD (Du et al, 2016;Kim et al, 2017;Reis et al, 2018;Cui et al, 2019;Zhang et al, 2020;Lin et al, 2022). Although these miRNAs have not been verified in the microenvironment of comorbidity with periodontitis and NAFLD, they also provide important therapeutic targets.…”

Diagnostic Significance of hsa-miR-21-5p, hsa-miR-192-5p, hsa-miR-155-5p, hsa-miR-199a-5p Panel and Ratios in Hepatocellular Carcinoma on top of Liver Cirrhosis in HCV Infected Patients

“…Correlation studies in all patients (n=79) revealed that plasma hsa-miR-21-5p expression level was positively correlated with serum insulin and the presence of insulin resistance, dyslipidemia (increased TC and TAG). Lin et al [26] suggested that altered plasma miRs might reflect liver lipid metabolism and stated that hepatic miRs expression contributes to the development of insulin resistance. This is per both hsa-miR-21-5p and hsa-miR-155-5p would control the expression of genes involved in hepatic TAG and cholesterol metabolism, evidenced by silencing hepatic MIR21 gene, where reduced hepatic inflammation and an enhanced fibrosis was achieved [27,28].…”