Circulating miR-1826 in plasma correlates with circulating tumor cells and is a prognostic marker in colorectal cancer

Abstract

Our previous study showed that miR-1826 was a newly identified oncogenic non-coding RNA in colorectal cancer. But the potential relationship between miR-1826 and tumor metastasis has not been fully elucidated. The purpose of this study was to evaluate the clinical significance of circulating miR-1826 and its possible associations with circulating tumor cells in colorectal cancer. Our results first found that serum miR-1826 was significantly upregulated in colorectal cancer patients, compared with that in healthy volunteers (p = 0.003). Similar results were also found in colorectal cancer with distant metastasis (p = 0.001) and advanced colorectal cancer (p < 0.001) patients, respectively. Clinicopathological analysis implied that circulating miR-1826 was positively associated with pT stage (p = 0.026), lymphatic metastasis (p = 0.034), distant metastasis (p = 0.012), and tumor-node-metastasis stage (p = 0.020). Besides, our univariate and multivariate analyses demonstrated that high serum miR-1826 expression could act as a prognostic and independent factor for overall survival of colorectal cancer patients (p < 0.05), which led to a poorer 5-year overall survival rate (p = 0.025). The area under the curve value of circulating miR-1826 was up to 0.848 ± 0.043, which strongly suggested serum miR-1826 as an effective diagnostic biomarker in colorectal cancer patients (p < 0.001). Our subsequent experiments demonstrated that patients with high level of circulating tumor cells showed a higher level of miR-1826 expression, compared with the circulating tumor cell-negative patients (p = 0.011). Similar results also showed that the amount of circulating tumor cells in high miR-1826 group was significantly higher than that in low miR-1826 group (p = 0.001). Furthermore, the relationship between serum miR-1826 and circulating tumor cells was analyzed using SPSS software and a significant logarithmic relationship was found, which meant that circulating miR-1826 closely correlated with the amount of circulating tumor cells in colorectal cancer patient serum (r = 0.283, p < 0.01). Our findings strongly suggested that serum miR-1826 could serve as an effective and non-invasive biomarker for diagnosis and prognosis of colorectal cancer. Circulating miR-1826 may be an important target in colorectal cancer therapy.

Downregulation of miR-1826 Indicates a Poor Prognosis for Osteosarcoma Patients and Regulates Tumor Cell Proliferation, Migration, and Invasion

“…Xue et al focused on the role of miR-638 in OS progression and demonstrated that miR-638 could enhance OS cell apoptosis and inhibit cell proliferation and invasion [22]. miR-1826 has been studied in some human cancers, such as melanoma [14], colorectal carcinoma [13], renal cancer [15], and bladder cancer [23]. The expression patterns of miR-1826 present a contradictory situation that the increased miR-1826 expression was found in colorectal carcinoma, while the decreased miR-1826 expression was observed in melanoma, renal cancer, and bladder cancer.…”

“…Chen et al reported that miR-191-5p was upregulated in OS tissues and associated with poor prognosis of OS patients [27]. For miR-1826, its aberrant expression has also been described as an indicator for the overall survival of colorectal carcinoma [13] and renal cancer [15]. According to the survival analysis in this study, the OS patients with low miR-1826 expression had a shorter survival time, and the downregulation of miR-1826 was determined as an independent prognostic indicator for the overall survival in OS patients, indicating that miR-1826 might serve as a novel biomarker for the prognosis of OS.…”

“…MicroRNA-1826 (miR-1826) has been studied in several cancers. In human melanoma and colorectal carcinoma, the deregulated miR-1826 has been identified as a biomarker for disease diagnosis and prognosis [13,14]. In renal cancer, miR-1826 played a tumor suppressor role by inhibiting tumor cell proliferation, migration, and invasion [15].…”