Circulating miRNA-3552 as a Potential Biomarker for Ischemic Stroke in Rats

Abstract

Objective. With the growing incidence of ischemic stroke worldwide, there is an urgent need to identify blood biomarkers for ischemic stroke patients. Thus, our aim was to identify potential circulating microRNA (miRNA) as a potential biomarker and to explore its potential mechanism for ischemic stroke in rats. Methods. The mRNA dataset GSE97537 and miRNA dataset GSE97532 were downloaded from the Gene Expression Omnibus (GEO) GSE97537 including 7 middle cerebral artery occlusion (MCAO) rat brain tissues and 5 sham-operated rat brain tissues GSE97532 including 6 MCAO rat blood samples and 3 sham-operated rat blood samples. Differentially expressed mRNAs and miRNAs with corrected p value ≤ 0.01 and fold change ≥2 or ≤0.05 were identified. To explore potential biological processes and pathways of differentially expressed mRNAs, functional enrichment analysis was performed. The target mRNAs of differentially expressed miRNAs were predicted using DNA Intelligent Analysis (DIANA)-microT tools. The target mRNAs and differentially expressed mRNAs were intersected. Results. 1228 differentially expressed mRNAs in MCAO rat brain tissues were identified. Highly expressed mRNAs were mainly enriched in the inflammatory responses. Nine differentially expressed miRNAs were identified in MCAO rat blood samples. A total of 673 target mRNAs were predicted to significantly bind these differentially expressed miRNAs. Among them, 54 target mRNAs were differentially expressed in MCAO rat blood samples. Enrichment analysis results showed that these 54 target mRNAs were closely related to neurological diseases and immune responses. Among all miRNA-mRNA relationship, miR-3552-CASP3 interaction was identified, indicating that CASP3 might be mediated by miR-3552. Functional enrichment analysis revealed that CASP3 was involved in the apoptosis pathway, indicating that miR-3552 might participate in apoptosis by CASP3. Conclusion. Our findings reveal that circulating miR-3552 shows promise as a potential biomarker for ischemic stroke in rats.

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“… 20 , 21 , 22 Aberrant expression of a miRNA was associated with ischemia stroke. 23 , 24 , 25 miR-125b-5p expression levels were upregulated in the brain tissue and blood plasma of mice 24 hours after ischemic stroke. 26 Therefore, we speculated that miR-125b-5p may regulate the neuroprotection in ischemia stroke.…”

Dysregulation Serum miR-19a-3p is a Diagnostic Biomarker for Asymptomatic Carotid Artery Stenosis and a Promising Predictor of Cerebral Ischemia Events

“… 24 Circulating miR-3352 may be a biomarker for ischemic stroke in rats. 25 The elevation of serum miR-374 in atherosclerosis is its diagnostic biomarker. 26 MiR-19a-3p is upregulated in pancreatic cancer and can be used as a diagnostic marker.…”

Effects of ischemic postconditioning and long non-coding RNAs in ischemic stroke

“…Progressive neuroinflammation not only damages brain cells, but also plays a positive role in nerve repair activities after cerebral ischemia. Studies have found that the differentially expressed circulating microRNA (miRNA)-3552 interacts with caspase-3 to participate in neuroinflammatory responses in ischemic stroke [ 12 ]. All stages of an ischemic episode, from cessation of cerebral blood flow to late recirculation of ischemic brain tissue, are accompanied by changes in neuroinflammation in the brain [ 13 ].…”