Circulating miRNAs as Biomarkers for Endometriosis and Endometriosis-Related Ovarian Cancer—An Overview

Moga, Marius; Bălan, Andreea; Dimienescu, Oana Gabriela; Burtea, Victoria; Dragomir, Roxana Maria; Anastasiu, Costin Vlad

doi:10.3390/jcm8050735

Cited by 57 publications

(34 citation statements)

References 113 publications

(127 reference statements)

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“…The incidence of the disease varies between 6-10% [2]. Various sources indicate a constant increase in the number of cases, reaching almost 15% worldwide [3]. Endometriosis is characterized by the migration of endometrial-like cells in ectopic places outside the uterus.…”

Section: Introductionmentioning

confidence: 99%

Biomarkers for the Noninvasive Diagnosis of Endometriosis: State of the Art and Future Perspectives

Anastasiu

Moga

Neculau

et al. 2020

IJMS

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126

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Background: Early and accurate diagnosis of endometriosis is crucial for the management of this benign, yet debilitating pathology. Despite the advances of modern medicine, there is no common ground regarding the pathophysiology of this disease as it continues to affect the quality of life of millions of women of reproductive age. The lack of specific symptoms often determines a belated diagnosis. The gold standard remains invasive, surgery followed by a histopathological exam. A biomarker or a panel of biomarkers is easy to measure, usually noninvasive, and could benefit the clinician in both diagnosing and monitoring the treatment response. Several studies have advanced the idea of biomarkers for endometriosis, thereby circumventing unnecessary invasive techniques. Our paper aims at harmonizing the results of these studies in the search of promising perspectives on early diagnosis. Methods: We selected the papers from Google Academic, PubMed, and CrossRef and reviewed recent articles from the literature, aiming to evaluate the effectiveness of various putative serum and urinary biomarkers for endometriosis. Results: The majority of studies focused on a panel of biomarkers, rather than a single biomarker and were unable to identify a single biomolecule or a panel of biomarkers with sufficient specificity and sensitivity in endometriosis. Conclusion: Noninvasive biomarkers, proteomics, genomics, and miRNA microarray may aid the diagnosis, but further research on larger datasets along with a better understanding of the pathophysiologic mechanisms are needed.

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Section: Introductionmentioning

confidence: 99%

Biomarkers for the Noninvasive Diagnosis of Endometriosis: State of the Art and Future Perspectives

Anastasiu

Moga

Neculau

et al. 2020

IJMS

Self Cite

126

View full text Add to dashboard Cite

show abstract

“…In addition, Wang et al [ 15 ] in a recent meta-analysis comprising a total of 40,609 cases of epithelial ovarian cancer and 368,452 controls from 38 publications, also found that endometriosis was associated with an increased risk of epithelial ovarian cancer (OR = 1.42, 95% CI = 1.28–1.57). Endometriosis and cancer present similarities [ 16 ], such as cell invasion, unrestrained growth, neo-angiogenesis, and decreased apoptosis [ 17 , 18 ], although the first condition is clearly not neoplastic.…”

Section: Introductionmentioning

confidence: 99%

BIRC5/Survivin Expression as a Non-Invasive Biomarker of Endometriosis

et al. 2020

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The etiology of endometriosis is highly complex, and although it is a benign disease, it has several biological behaviors similar to malignant lesions, including cell invasion, neo-angiogenesis, and decreased apoptosis. Survivin is a protein encoded by the BIRC5 gene that plays a role in cell division by inhibiting apoptosis and regulating the process of mitosis in embryonic and cancer cells. Therefore, we aimed to evaluate the expression of BIRC5 in samples of peripheral blood of women with and without endometriosis. This study comprised of 40 women with endometriosis and 10 healthy women as controls. Peripheral blood samples were collected in the three phases of the menstrual cycle (follicular, ovulatory, and luteal). The expression of the BIRC5 gene was evaluated by RT-qPCR using the TaqMan methodology. The BIRC5 expression was significantly higher in all phases of the menstrual cycle in women with endometriosis, regardless of the disease stage. The accuracy of BIRC5 expression in the peripheral blood for the diagnosis endometriosis presented AUC of 0.887 (p < 0.001), with 97.2% of sensitivity and specificity of 65.5% considering the overall endometriosis group. Regarding the minimal/mild endometriosis group, the AUC presented a value of 0.925 (p < 0.001), with 100% of sensitivity and 79.3% of specificity, whereas in the moderate/severe endometriosis group the AUC was 0.868 (p < 0.001), with a sensitivity of 95.8% and specificity of 65.5%. These findings suggest that the expression of BIRC5 may be a potential noninvasive biomarker for the diagnosis of endometriosis.

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“…Substantial numbers of ncRNAs including microRNAs (miRNAs) and long noncoding RNAs (lncRNAs) possess abilities to affect the development and persistence of EMS, unraveling that is of great importance and value to determine novel therapeutic targets and strategies [11]. The miRNAs are a group of short, single-stranded RNAs that control mRNA post-transcriptionally [12].The members of miR-200 family, such as miR-143, 145, miR-20a and miR199a have been reported as the most commonly abnormally expressed miRNAs in EMS [13]. miR-20a is identified as a miRNA that could mediate endothelial and endometrial cell proliferation by inducing fibroblast growth factor-9 [14].…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-20a Mediates the Cytotoxicity of Natural Killer Cells in Endometriosis <i>via</i> ERG/HLX/STAT4/Perforin Axis

Chen¹,

Hu²,

Han³

et al. 2020

SSRN Journal

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Background: Intriguingly, microRNA-20a (miR-20a) has been recently witnessed to be involved in the pathogenesis of endometriosis (EMS) but the molecular mechanism controlled by miR-20a is to be undefined. The present study is designed to probe into how miR-20a acts to regulate the cytotoxicity of natural killer (NK) cells. Methods: Most of all, consistent with the clinical determination in EMS patients, miR-20a was determined to be down-regulated in NK cells isolated from nude mice. miR-20a could specifically bind to ERG and negatively regulates its expression in NK cells. Additionally, shRNA-mediated silencing of ERG decreased the expression of HLX. HLX up-regulated STAT4 by inducing proteasome degradation and inhibited NK cell cytotoxicity. Results: Of great importance, forced expression of miR-20a consequently induced NK cell cytotoxicity in vitro by increasing perforin expression via enhancement of STAT4 that was caused by impairing the binding of ERG to HLX enhancer. The in vivo experiments further confirmed the promoting role of miR-20a in the cytotoxicity of NK cells isolated from EMS nude mice and subsequent protective role of miR-20a against EMS-induced endometrial injury. Conclusion: The aforementioned data suggest that miR-20a potentiates the cytotoxicity of NK via upregulating perforin mediated by ERG/HLX/STAT4, highlighting potential novel mechanisms associated with EMS progression.

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Circulating miRNAs as Biomarkers for Endometriosis and Endometriosis-Related Ovarian Cancer—An Overview

Cited by 57 publications

References 113 publications

Biomarkers for the Noninvasive Diagnosis of Endometriosis: State of the Art and Future Perspectives

Biomarkers for the Noninvasive Diagnosis of Endometriosis: State of the Art and Future Perspectives

BIRC5/Survivin Expression as a Non-Invasive Biomarker of Endometriosis

MicroRNA-20a Mediates the Cytotoxicity of Natural Killer Cells in Endometriosis <i>via</i> ERG/HLX/STAT4/Perforin Axis

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