Circulating neutrophils may play a role in the host response in cervical cancer

Abstract

Neutrophil migration is a key event in the inflammatory response of any origin, and neutrophils may present antitumor activity. We investigated the number and function of circulating neutrophils obtained from patients with cervical neoplasia at different stages. Patients with preinvasive (cervical intraepithelial neoplasia, CIN3, n= 6) or microinvasive ([MICRO] stage IA1, n= 4) neoplasia were evaluated together as CIN/MICRO group (n= 10), while patients at stages II-IV were evaluated as invasive group (INV, n= 12). Healthy women served as controls (n= 15). For patients, analysis of leukogram on diagnosis showed a significant elevated neutrophil count in INV group compared with that in CIN/MICRO group. A neutrophil/lymphocyte ratio >/=5 was observed in 67% patients from INV group compared with only 10% from CIN/MICRO group. Neutrophil migration, assayed in a microchemotaxis chamber in response to the chemoattractants (10(-7) M) N-formyl-l-methionyl-l-leucyl-l-phenylalanine, leukotriene B(4), or interleukin-8, was reduced in INV group than in controls or CIN/MICRO group. Surgical treatment in randomly selected patients from CIN/MICRO group (four CIN, one MICRO) increased neutrophil migration to all chemoattractants compared with time on diagnosis. The serum levels of nitric oxide (NO) metabolites, assayed by the Griess reaction, were higher in patients (n= 19) than in controls (n= 15), without differences related to tumor stage, but were reduced in patients after surgery compared with pretreatment (n= 10). Taken together, the results suggest that neutrophils play a role in the host response in cervical cancer. Soluble circulating mediators released by tumor cells, such as NO, could interfere early in the capacity of neutrophils to migrate, thus impairing host immune response.

Prognostic Use of Pretreatment Hematologic Parameters in Patients Receiving Definitive Chemoradiotherapy for Cervical Cancer

“…In cervical cancer, elevated neutrophil counts, fewer lymphocytes, or high NLR have proved to be significantly predictive of survival. 10,13,19,30,31 Whereas Wang et al 32 failed to demonstrate the value of pretreatment NLR in predicting survival of patients subjected Lee et al 10 also analyzed the prognostic value of NLR in 1,061 patients with cervical cancer (early stage, 82%) who were variably treated and followed for a median of 53 months. Patients with higher NLR were younger and had more advanced disease.…”

Preoperative Neutrophil-Lymphocyte Ratio Before Platelet-Lymphocyte Ratio Predicts Clinical Outcome in Patients With Cervical Cancer Treated With Initial Radical Surgery

“…These studies include colorectal cancer, 12 gastric cancer treated with FOLFOX chemotherapy 10 or neoadjuvant chemotherapy, 13 and small hepatocellular carcinoma undergoing radiofrequency ablation. 14 In cervical cancer, some reports showed that low circulating lymphocytes, 15 elevated levels of neutrophil counts, 16 or high pretreatment NLR 17 is a risk indicator for prognosis. However, Wang et al 18 found that pretreatment NLR and PLR failed to predict the survival of patients with cervical cancer treated with neoadjuvant chemotherapy and radical hysterectomy.…”

Glucocorticoid-induced tumor necrosis factor receptor expression in patients with cervical human papillomavirus infection

“…However, it has been observed that persistent infection with a higher likelihood of progression to high-grade lesions and invasive carcinoma can occur in the face of an ineffective immune response. In this context and considering that HPV infection is restricted to epithelial cells, the importance of the local immune response is highlighted, making the components present in the microenvironment crucial for lesion development or regression [33][34][35][36] . The role of GITR has been unclear until now, emphasizing the importance of the present study to clarify the immune response in the cervical microenvironment.…”