Circulating oxidized LDL: determinants and association with brachial flow-mediated dilation

Zwan, Leonard P. van der; Teerlink, Tom; Dekker, Joost; Henry, Ronald M.A.; Stehouwer, Coen D.A.; Jakobs, Cornelis; Heine, Robert J.; Scheffer, P

doi:10.1194/jlr.p800030-jlr200

Cited by 40 publications

(39 citation statements)

References 33 publications

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“…Using the same ELISA kit as our study, it has been reported that ox-LDL significantly correlates with LDL-cholesterol [34,41]. In our study, we were unable to show this association in any of our three groups.…”

Section: Discussioncontrasting

confidence: 80%

“…It can be hypothesized that, alongside with the progression of the inflammatory status, ox-LDL increases with the chronicity of diabetes [33]. It was shown that ox-LDL is associated with hypercholesterolemia and high serum LDL-cholesterol [34,35]. Surprisingly, in our results, we found a significant increase in the ox-LDL level in parallel with a decrease in the LDL-cholesterol level in patients with prolonged diabetes.…”

Section: Discussioncontrasting

confidence: 64%

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Serum Oxidized‐LDL is Associated with Diabetes Duration Independent of Maintaining Optimized Levels of LDL‐Cholesterol

Nakhjavani

Khalilzadeh

Khajeali

et al. 2010

Lipids

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Oxidized low-density lipoprotein (ox-LDL) plays a key role in the progression of atherosclerosis and diabetes complications. The aim of this study was first, to evaluate the association between ox-LDL and diabetes duration, and second, to examine serum level of ox-LDL in patients with prolonged diabetes and a desirable LDL-cholesterol level. A total of 36 type-2 diabetic patients with a diabetes duration of more than 5 years, 36 newly diagnosed diabetic patients, and 36 age-, sex- and BMI-matched healthy participants were recruited. Healthy participants and newly diagnosed patients were not receiving any treatment. All patients with prolonged diabetes had desirable LDL-cholesterol levels (<100 mg/dL), according to the adult treatment panel-III guidelines. While LDL-cholesterol was significantly lower in patients with diabetes duration >5 years, in comparison to newly diagnosed patients (P < 0.01), ox-LDL was significantly higher in patients with prolonged diabetes (P < 0.001). The ox-LDL-to-LDL ratio was dramatically higher in patients with diabetes duration >5 years in comparison to newly diagnosed patients and healthy participants (P < 0.001). Ox-LDL was significantly associated with diabetes duration (r = 0.519, P = 0.001). In multivariate analysis, this association remained significant (beta = 0.501, P = 0.003) after adjustment for potential confounders. In conclusion, this study showed that the serum ox-LDL level increases with the length of diabetes, even though the patients' LDL-cholesterol level is maintained at a desirable level. Our findings highlight that possibly more attention should be focused on markers of oxidative stress in the management of lipids in diabetic patients.

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Section: Discussioncontrasting

confidence: 80%

Section: Discussioncontrasting

confidence: 64%

Serum Oxidized‐LDL is Associated with Diabetes Duration Independent of Maintaining Optimized Levels of LDL‐Cholesterol

Nakhjavani

Khalilzadeh

Khajeali

et al. 2010

Lipids

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“…In our study, we found that HDL-c was a negative determinant of oxLDL, likes Navab et al [20] der Zwan et al [33]. There are several hypotheses about two possible mechanisms could contribute to the protective role of HDL in the formation of oxLDL [10,13,15,23]; they are related to two HDLassociated enzymes that have been shown to inhibit LDL lipid-peroxidation in vitro: Paraoxonase 1 [16] and platelet-activating factor acetylhydrolase [35], respectively.…”

Section: Discussionsupporting

confidence: 61%

Oxidized LDL and its correlation with lipid profile and oxidative stress biomarkers in young healthy Spanish subjects

et al. 2010

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The biological effects of oxidized LDL (oxLDL) may contribute to initiation and progression of the atherosclerotic process, and the association between cardiovascular disease and oxidation of LDL has been largely demonstrated. The objectives of this study were to establish the reference values of oxidative stress biomarkers in a young healthy Spanish population to determine the concentration of oxLDL and its relationship with lipid profile and with these biomarkers. oxLDL, F(2)-isoprostanes, protein carbonyls (PC), and 8-hydroxy-2'-deoxyguanosine (8-OHdG) were determinate by ELISA in 72 healthy subjects. Antioxidant enzymes, superoxide dismutase (SOD), glutathione peroxidase (GPx), and glutathione reductase (GR) were carried out on a Hitachi 912 analyzer; lipid profile were assayed using automated systems (Cobas 711, Roche Diagnostics). All statistics were analyzed by using SPSS for Windows 15.0. SPSS Inc, Chicago, IL, USA. (Normal mean reference values): oxLDL (63.23 +/- 16.23 U/L), (Male/Female 68.06 +/- 17.69/58.39 +/- 13.6 U/L), F(2)-isoprostanes (2.26 +/- 0.9 microg/g creatinine), PC (0.34 +/- 0.15 nmol/mg), 8-OHdG (23.27 +/- 10.58 ng/ml), SOD (931.97 +/- 271.09 U/g Hb), GR (46.56 +/- 11.68 U/L), GPx (27.58 +/- 6.89 U/gHb (Male/Female 25.91 +/- 5.03/29.2 +/- 8.07 U/L)). OxLDL (63.23 U/L) was significantly (p < 0.05) positively correlated with BMI (22.53 Kg/m(2)), total cholesterol (175.79 mg/dl), triglycerides (87.58 mg/dl), LDL cholesterol (96.25 mg/dl), and uric acid (4.78 mg/dl), while negatively correlated with HDL-cholesterol (62.25 mg/dl). We have found different correlation between oxLDL and isoprostanes by gender with the rest of parameters. Normal reference values have been found significantly different for oxLDL and GPx by gender. Oxidized LDL is correlated with lipid profile but not with the oxidative stress biomarkers. Urinary isoprostanes are positively correlated with triglycerides and negatively with GR and GPx.

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“…16 Circulating plasma oxidized low-density lipoprotein (LDL) was determined by competitive enzyme-linked immunosorbent assay (Mercodia, Uppsala, Sweden). 17 Hemoglobin A1c (HbA1c) was analyzed by ion-exchange high-performance liquid chromatography (reference range, 4.3 to 6.1%) on a modular monitoring system (Bio-Rad, Veenendaal, The Netherlands). Glucose was measured enzymatically (Roche, Mannheim, Germany), and insulin was determined by a 2-site immunoradiometric assay (Medgenix Diagnostics, Fleurus, Belgium).…”

Section: Biochemical Analysesmentioning

confidence: 99%

Hyperglycemia and Oxidative Stress Strengthen the Association Between Myeloperoxidase and Blood Pressure

et al. 2010

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Abstract-Scavenging of the vasodilator nitric oxide by myeloperoxidase activity in the vasculature may contribute to hypertension. Because hydrogen peroxide is a cosubstrate of myeloperoxidase, hyperglycemia-induced oxidative stress may strengthen the relationship between myeloperoxidase and blood pressure. We investigated this relationship and its modification by hyperglycemia and oxidative stress in a population-based cohort of elderly subjects with normal glucose metabolism (nϭ267), impaired glucose metabolism (nϭ189), and type 2 diabetes (nϭ290 Many studies have demonstrated that systemic MPO levels predict risk throughout the spectrum of cardiovascular diseases. 4 -8 MPO catalyzes the production of hypochlorous acid and a range of other highly reactive species. These MPOderived reactive substances may damage the arterial wall, thereby reducing its elasticity. In addition, by several mechanisms, MPO reduces the bioavailability of the endogenous vasodilator nitric oxide. 3,9 Together, these mechanisms may lead to an increase in blood pressure. Because hydrogen peroxide is an obligate cosubstrate of MPO, the activity of MPO in the vasculature may be enhanced by increased local production of reactive oxygen species. Vascular production of superoxide and its dismutation product hydrogen peroxide has been shown to be stimulated by high glucose concentrations, resulting in increased activity of MPO. 10 We therefore hypothesize that the relationship between MPO and blood pressure is stronger on a background of (hyperglycemiainduced) oxidative stress.The aims of our study were to assess the relationship between plasma levels of MPO and blood pressure and to test the hypothesis that this relationship is strengthened by (hyperglycemia-induced) oxidative stress. Methods SubjectsThe present study was conducted in the Hoorn Study 11 follow-up examination conducted in 2000 and the Hoorn Screening Study, 12 both of which are population-based studies in a white population. From the 822 participants, we excluded subjects with missing data on primary variables of interest, leaving 746 subjects, of whom 267

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Circulating oxidized LDL: determinants and association with brachial flow-mediated dilation

Cited by 40 publications

References 33 publications

Serum Oxidized‐LDL is Associated with Diabetes Duration Independent of Maintaining Optimized Levels of LDL‐Cholesterol

Serum Oxidized‐LDL is Associated with Diabetes Duration Independent of Maintaining Optimized Levels of LDL‐Cholesterol

Oxidized LDL and its correlation with lipid profile and oxidative stress biomarkers in young healthy Spanish subjects

Hyperglycemia and Oxidative Stress Strengthen the Association Between Myeloperoxidase and Blood Pressure

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