Circulating Oxidized LDL Is a Useful Marker for Identifying Patients With Coronary Artery Disease

Holvoet, Paul; Mertens, Ann; Verhamme, Peter; Bogaerts, Kris; Beyens, Guy; Verhaeghe, Raymond; Collen, Désiré; Muls, Erik; Werf, Frans Van de

doi:10.1161/01.atv.21.5.844

Cited by 474 publications

(352 citation statements)

References 37 publications

(26 reference statements)

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“…The trend would be established on including a large number of subjects. Recent studies by Holvoet et al have shown that CAD patients had a higher level of circulating ox-LDL as compared to controls and the sensitivity of this circulating ox-LDL was 76% for cases of CAD (16). It has also been seen that patients with CAD have elevated titre of autoantibodies against ox-LDL as compared to healthy…”

Section: Controls (17)mentioning

confidence: 99%

Oxidised LDL, HDL cholesterol, LDL cholesterol levels in patients of coronary artery disease

Ghosh

Mishra

Rao

et al. 2006

Indian J Clin Biochem

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Coronary artery disease is a major cause of morbidity and has various risk factors. Lipid profile i.e. low HDL-cholesterol, high LDL cholesterol, high total cholesterol, high triglycerides playing important role in its causation. Recently interest has been shown in the oxidized fraction of LDL as one of the risk factors. In the present study 60 age and sex matched normal healthy individuals were taken as controls and 60 patients of CAD were taken. Cholesterol was measured by enzymatic method, HDL cholesterol by phosphotungstate precipitation method. Serum levels of LDL fraction of cholesterol was measured by a new and simpler method of precipitation. Result was expressed as mol/L of diene conjugates. It was observed that LDL cholesterol, VLDL cholesterol, total cholesterol, total cholesterol:HDL cholesterol, LDL cholesterol:HDL cholesterol were significantly raised and HDL cholesterol was significantly low in patients. (p< 0.001). Though HDL cholesterol was significantly raised in females as compared to males in both the groups (p<0.001). Serum level of total cholesterol, oxidized LDL:HDL cholesterol were also raised significantly (p<0.05). The level of oxidized LDL showed an increasing trend in patients.

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Section: Controls (17)mentioning

confidence: 99%

Oxidised LDL, HDL cholesterol, LDL cholesterol levels in patients of coronary artery disease

Ghosh

Mishra

Rao

et al. 2006

Indian J Clin Biochem

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“…A monoclonal antibody (4E6) against a conformational epitope in the ApoB-100 moiety of LDL was used (Holvoet et al, 1998(Holvoet et al, , 2001). The interassay coefficient variation was p7%.…”

Section: Standard Assaysmentioning

confidence: 99%

Effects of marine n-3 fatty acid supplementation on lipoprotein subclasses measured by nuclear magnetic resonance in subjects with type II diabetes

et al. 2007

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Objective: To measure effects of fish oil supplements on lipoprotein subclasses by nuclear magnetic resonance (NMR) in subjects with type II diabetes and relate them to insulin sensitivity. Design: Two-armed, parallel, placebo-controlled, randomized. Subjects: Normotriglyceridemic subjects with type II diabetes without insulin treatment were given either fish oil (n ¼ 12, median intake 5.9 g/day total n-3 fatty acids (FA) (1.8 g 20:5n-3, 3.0 g 22:6n-3)) or corn oil (n ¼ 14, 8.5 g/day 18:2n-6 FA). Methods: Size and concentration of lipoproteins subclasses were measured by NMR, insulin sensitivity by hyperinsulinemic, isoglycemic clamps. Results: After 9 weeks, there were differences between those treated with fish and corn oil with respect to very low-density lipoprotein (VLDL) size (median À15 vs þ 0.6%, P ¼ 0.001), particle concentrations of large VLDL (À99 vs À4.1%, P ¼ 0.041) and small high-density lipoprotein (HDL) (À12 vs þ 10%, P ¼ 0.051). Compared with corn oil fish oil tended to increase HDL size and small low-density lipoprotein ( LDL) concentration (P ¼ 0.063 and 0.068, respectively, for differences between groups). There was no effect on oxidized LDL. Insulin sensitivity (glucose utilization) decreased in the fish oil group compared with the corn oil group (P ¼ 0.049). The decrease in insulin sensitivity did not correlate with the effects on lipoprotein subclasses. Conclusions: A high intake of n-3 FA exerts effects on several lipoprotein subclasses without obvious influence from changes in insulin sensitivity.

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“…30, 31 We have shown that dyslipidemia in association with obesity is an independent predictor of circulating oxidized LDL in persons without clinical evidence of cardiovascular disease. 6 Because the assay for oxidized LDL in blood is based on a mouse monoclonal antibody, oxidized LDL cannot be measured directly in mouse blood. Previously, the titer of autoantibodies against MDA-modified LDL has been used as a proxy for oxidized LDL in mice.…”

Section: Oxidation Of Ldl and Macrophage Accumulationmentioning

confidence: 99%

“…However, the increased oxidative stress in obese dyslipidemic mice is in agreement with the increased oxidation of LDL in obese people. 6 …”

Section: Limitations Of the Studymentioning

confidence: 99%

“…We have investigated the effect of hypercholesterolemia and insulin resistance on LDL oxidation because we have demonstrated increased LDL oxidation in obese subjects that are at high risk for cardiovascular disease. 6 We have particularly investigated the cholesterol acceptor and antioxidant function of high-density lipoprotein (HDL). 7 It is well-known that HDL protects against cardiovascular disease.…”

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confidence: 99%

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Increased Low-Density Lipoprotein Oxidation and Impaired High-Density Lipoprotein Antioxidant Defense Are Associated With Increased Macrophage Homing and Atherosclerosis in Dyslipidemic Obese Mice

et al. 2003

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Background-Obesity-associated dyslipidemia in humans is associated with increased low-density lipoprotein (LDL) oxidation. Mice with combined leptin and LDL receptor deficiency are obese and show severe dyslipidemia and insulin resistance. We investigated the association between oxidation of apolipoprotein B-containing lipoproteins, high-density lipoprotein (HDL) antioxidant defense, and atherosclerosis in these mice. Methods and Results-LDL receptor knockout (LDLR Ϫ/Ϫ ), leptin-deficient (ob/ob), double-mutant (LDLR Ϫ/Ϫ ;ob/ob), and C57BL6 mice were fed standard chow. Double-mutant mice had higher levels of non-HDL (PϽ0.001) and HDL (PϽ0.01) cholesterol and of triglycerides (PϽ0.001). They also had higher oxidative stress, evidenced by higher titers of autoantibodies against malondialdehyde-modified LDL (PϽ0.001). C57BL6 and ob/ob mice had no detectable lesions. Lesions covered 20% of total area of the thoracic abdominal aorta in double-mutant mice compared with 3.5% in LDLR Ϫ/Ϫ mice (PϽ0.01). Higher macrophage homing and accumulation of oxidized apolipoprotein B-100 -containing lipoproteins were associated with larger plaque volumes in the aortic root of double-mutant mice (PϽ0.01). The activity of the HDL-associated antioxidant enzymes paraoxonase and lecithin:cholesterol acyltransferase (LCAT) (ANOVA; PϽ0.0001 for both) was lower in double-mutant mice. Adenovirus-mediated LCAT gene transfer in double-mutant mice increased plasma LCAT activity by 64% (PϽ0.01) and reduced the titer of autoantibodies by 40% (PϽ0.01) and plaque volume in the aortic root by 42% (PϽ0.05) at 6 weeks. Conclusions-Dyslipidemia and insulin resistance in obese LDL receptor-deficient mice are associated with increased oxidative stress and impaired HDL-associated antioxidant defense, evidenced by decreased paraoxonase and LCAT activity. Transient LCAT overexpression was associated with a reduction of oxidative stress and atherosclerosis.

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Circulating Oxidized LDL Is a Useful Marker for Identifying Patients With Coronary Artery Disease

Cited by 474 publications

References 37 publications

Oxidised LDL, HDL cholesterol, LDL cholesterol levels in patients of coronary artery disease

Oxidised LDL, HDL cholesterol, LDL cholesterol levels in patients of coronary artery disease

Effects of marine n-3 fatty acid supplementation on lipoprotein subclasses measured by nuclear magnetic resonance in subjects with type II diabetes

Increased Low-Density Lipoprotein Oxidation and Impaired High-Density Lipoprotein Antioxidant Defense Are Associated With Increased Macrophage Homing and Atherosclerosis in Dyslipidemic Obese Mice

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