Circulating PAMM, a novel antioxidant and anti-inflammatory protein, is elevated in acute SCI

Abstract

Background: Peroxiredoxin activated in M-CSF stimulated monocytes (PAMM) is a novel protein produced by adipocytes with putative redox regulatory and anti-inflammatory properties. Because acute spinal cord injury (SCI) is associated with oxidative stress and neuroinflammation and because PAMM can be detected in systemic circulation, we hypothesized that acute neuro-trauma might induce changes in circulating PAMM expression. Specifically, we hypothesized that PAMM levels might vary based on the presence or absence of acute, traumatic SCI. We therefore investigated circulating PAMM levels in adults with and without acute traumatic SCI. Methods:We studied 105 men and women (54 with SCI and 51 without SCI). Participants with SCI were admitted for acute rehabilitation within 1 month after injury. Serum samples were obtained during hospitalization and stored at − 80 °C until batch analysis. Total PAMM was quantified by ELISA assay (MyBiosource, Cat. No: MBS9327247) with a detection limit of 0.25 ng/ml. Separate multivariate models including age, BMI, and injury severity were assessed to determine significant clinical predictors of change in PAMM levels.Results: When adjusting for BMI, age, and gender, mean change in PAMM levels were greatest in participants with motor complete SCI compared to able-bodied (1.65 ng/ml versus 0.94 ng/ml, p = 0.003). This model explained 26% of the variation in change in circulating PAMM levels. Conclusions:Our results suggest that PAMM may be a novel biomarker of neurological injury or of native antiinflammatory responses to neurological injury. More work is needed to establish the role of PAMM and other adipocyte-derived factors in the acute response to neurotrauma.

Unveiling Human Proteome Signatures of Heart Failure with Preserved Ejection Fraction

“…The upregulation of peroxiredoxin-like 2A (PRXL2A, also known as PAMM, or FAM213A, Table 2 ) points to the negative regulation of inflammation in the myocardium of HFpEF patients. In addition, PRXL2A has been reported to act as a negative regulator of macrophage-induced inflammation [ 23 ] and is also involved in protection against cellular oxidative stress [ 24 , 25 ].…”

Anti-inflammatory and antioxidative effects of genistein in a model of spinal cord injury in rats

“…Oxidative stress is an ongoing process throughout both primary and secondary damage, and the severity and uncontrolled nature of oxidative stress are related to the magnitude of the damage [ 1 , 4 , 5 , 6 ]. Thus, markers of oxidative stress have been used to monitor SCI and response to treatment [ 7 , 8 , 9 ], and many studies have focused on resolving inflammation and antioxidant therapies for traumatic brain and SCI [ 6 , 10 ].…”