Circulating tumor cells detected with a microcavity array predict clinical outcome in hepatocellular carcinoma

Takahashi, Kazuto; Ofuji, Kazuya; Hiramatsu, Katsushi; Nosaka, Takuto; Naito, Tateaki; Matsuda, Hidetaka; Endo, Katsuya; Higuchi, Makie; Ohtani, Masahiro; Nemoto, Tomoyuki; Nakamoto, Yasunari

doi:10.1002/cam4.3790

Cited by 15 publications

(9 citation statements)

References 26 publications

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“…In contrast, positive-enrichment methods take advantage of specific biological and physical properties of CTCs to distinguish them from non-tumoral blood cells. Physical enrichment strategies are frequently based on centrifugation and microfiltration [ 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 ] to selectively isolate CTCs according to their differential density and size, respectively [ 51 ]. Among all the positive-enrichment methods, immunoaffinity is the most widely used but the optimal biomarker matching all CTC subpopulations is unknown.…”

Section: Liquid Biopsy and Ctcsmentioning

confidence: 99%

Circulating Tumor Cells in Hepatocellular Carcinoma: A Comprehensive Review and Critical Appraisal

Espejo-Cruz

González-Rubio

Olaya

et al. 2021

IJMS

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Hepatocellular carcinoma (HCC) is the fifth most common neoplasm and a major cause of cancer-related death worldwide. There is no ideal biomarker allowing early diagnosis of HCC and tumor surveillance in patients receiving therapy. Liquid biopsy, and particularly circulating tumor cells (CTCs), have emerged as a useful tool for diagnosis and monitoring therapeutic responses in different tumors. In the present manuscript, we evaluate the current evidence supporting the quantitative and qualitative assessment of CTCs as potential biomarkers of HCC, as well as technical aspects related to isolation, identification, and classification of CTCs. Although the dynamic assessment of CTCs in patients with HCC may aid the decision-making process, there are still many uncertainties and technical caveats to be solved before this methodology has a true impact on clinical practice guidelines. More studies are needed to identify the optimal combination of surface markers, to increase the efficiency of ex-vivo expansion of CTCs, or even to target CTCs as a potential therapeutic strategy to prevent HCC recurrence after surgery or to hamper tumor progression and extrahepatic spreading.

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Section: Liquid Biopsy and Ctcsmentioning

confidence: 99%

Circulating Tumor Cells in Hepatocellular Carcinoma: A Comprehensive Review and Critical Appraisal

Espejo-Cruz

González-Rubio

Olaya

et al. 2021

IJMS

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“…The chip is composed of a row of posts coated with antibodies. The larger cancer cells collide with the row of posts and stick on as the other cells flow through [33] The OncoCEE ® (Biocept, Inc.)…”

Section: Technology Detection Methods Description Referencesmentioning

confidence: 99%

“…The isolation and capture of CTCs based on biological characteristics of those heterogeneous cells utilize different biological markers expressed in the cells ( Table 2 ) [ 18 , 19 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 45 , 56 , 57 , 58 , 59 , 60 , 61 , 62 ]. The first data describing the detection and characterization of carcinoma cells in the systemic circulation (bone marrow) based on cytokeratin and MUC-1 glycoprotein expression in epithelial cancer cells, but not in healthy cells, were published 25 years ago [ 63 ].…”

Section: Ctc Isolation and Detection Techniquesmentioning

confidence: 99%

Circulating Tumor Cells from Enumeration to Analysis: Current Challenges and Future Opportunities

Yang

Giret

Côté

2021

Cancers

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Circulating tumor cells (CTCs) have been recognized as a major contributor to distant metastasis. Their unique role as metastatic seeds renders them a potential marker in the circulation for early cancer diagnosis and prognosis as well as monitoring of therapeutic response. In the past decade, researchers mainly focused on the development of isolation techniques for improving the recovery rate and purity of CTCs. These developed techniques have significantly increased the detection sensitivity and enumeration accuracy of CTCs. Currently, significant efforts have been made toward comprehensive molecular characterization, ex vivo expansion of CTCs, and understanding the interactions between CTCs and their associated cells (e.g., immune cells and stromal cells) in the circulation. In this review, we briefly summarize existing CTC isolation technologies and specifically focus on advances in downstream analysis of CTCs and their potential applications in precision medicine. We also discuss the current challenges and future opportunities in their clinical utilization.

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“…Several research groups have successfully developed microfluidic devices for CTC capture. 16,32,40,42,43 For example, Yin et al reported a pyramidal microcavity array (8 μm wide rectangular pores) for CTC capture, and the capture efficiency was about 80%. 42 Negishi et al presented a microcavity array (8 μm wide rectangular pores) that can separate CTCs with a capture efficiency of ∼95%.…”

Section: Introductionmentioning

confidence: 99%

Ultrathin silicon nitride membrane with slit-shaped pores for high-performance separation of circulating tumor cells

et al. 2022

Lab Chip

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Circulating tumor cells detected with a microcavity array predict clinical outcome in hepatocellular carcinoma

Abstract: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 15 publications

References 26 publications

Circulating Tumor Cells in Hepatocellular Carcinoma: A Comprehensive Review and Critical Appraisal

Circulating Tumor Cells in Hepatocellular Carcinoma: A Comprehensive Review and Critical Appraisal

Circulating Tumor Cells from Enumeration to Analysis: Current Challenges and Future Opportunities

Ultrathin silicon nitride membrane with slit-shaped pores for high-performance separation of circulating tumor cells

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