Circulating tumour cells: The Good, the Bad and the Ugly

Bates, Mark; Mohamed, Bashir M.; Ward, Mark P.; Kelly, Tanya; O’Connor, Roisin M.; Malone, Victoria; Brooks, Robert D.; Brooks, Doug A.; Selemidis, Stavros; Martin, Cara; O’Toole, Sharon; O’Leary, John

doi:10.1016/j.bbcan.2023.188863

Cited by 18 publications

(6 citation statements)

References 134 publications

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“…20 This conventional definition has inevitably ignored CD45 positive cells with cancer cell characteristics. 2,9 To determine whether CD45 + CTCs exist in the circulation of cancer patients, we first isolated CTCs from the peripheral blood of colorectal cancer (CRC) patients using Cellab Thomas I CTCs processing workstation (Fig. 1a).…”

Section: Resultsmentioning

confidence: 99%

“…8 The conventional definition of CTCs in human is a circulating cell expressing a tumor antigen (typically the epithelial cell adhesion molecule (EpCAM) or cytokeratin (CK) for epithelial cancers) but not expressing the panleukocyte antigen CD45. 2,9 CD45, also known as protein tyrosine phosphatase receptor type C (PTPRC) or leucocyte common antigen (LCA), is expressed on almost all nucleated blood cells. It plays a crucial role in regulating the signaling thresholds of immune cells.…”

Section: Introductionmentioning

confidence: 99%

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Circulating tumor cells shielded with extracellular vesicle-derived CD45 evade T cell attack to enable metastasis

Yang,

Wang,

et al. 2024

Sig Transduct Target Ther

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Circulating tumor cells (CTCs) are precursors of distant metastasis in a subset of cancer patients. A better understanding of CTCs heterogeneity and how these CTCs survive during hematogenous dissemination could lay the foundation for therapeutic prevention of cancer metastasis. It remains elusive how CTCs evade immune surveillance and elimination by immune cells. In this study, we unequivocally identified a subpopulation of CTCs shielded with extracellular vesicle (EVs)-derived CD45 (termed as CD45+ CTCs) that resisted T cell attack. A higher percentage of CD45+ CTCs was found to be closely correlated with higher incidence of metastasis and worse prognosis in cancer patients. Moreover, CD45+ tumor cells orchestrated an immunosuppressive milieu and CD45+ CTCs exhibited remarkably stronger metastatic potential than CD45− CTCs in vivo. Mechanistically, CD45 expressing on tumor surfaces was shown to form intercellular CD45-CD45 homophilic interactions with CD45 on T cells, thereby preventing CD45 exclusion from TCR-pMHC synapse and leading to diminished TCR signaling transduction and suppressed immune response. Together, these results pointed to an underappreciated capability of EVs-derived CD45-dressed CTCs in immune evasion and metastasis, providing a rationale for targeting EVs-derived CD45 internalization by CTCs to prevent cancer metastasis.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Circulating tumor cells shielded with extracellular vesicle-derived CD45 evade T cell attack to enable metastasis

Yang,

Wang,

et al. 2024

Sig Transduct Target Ther

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“…Subsequently, they interact with a diverse array of cell types within the circulation [31]. Ultimately, these cells adhere to endothelial membranes and extravasate to distant organs, instigating the development of secondary tumors [32]. Physicochemical characteristics or cell surface molecules are commonly employed for the isolation of CTCs from normal blood cells [33].…”

Section: Definition and Characteristics Of Ctcsmentioning

confidence: 99%

Exploring the Immunological Profile in Breast Cancer: Recent Advances in Diagnosis and Prognosis through Circulating Tumor Cells

Kotsifaki,

Maroulaki,

Armakolas

2024

IJMS

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This review offers a comprehensive exploration of the intricate immunological landscape of breast cancer (BC), focusing on recent advances in diagnosis and prognosis through the analysis of circulating tumor cells (CTCs). Positioned within the broader context of BC research, it underscores the pivotal role of the immune system in shaping the disease’s progression. The primary objective of this investigation is to synthesize current knowledge on the immunological aspects of BC, with a particular emphasis on the diagnostic and prognostic potential offered by CTCs. This review adopts a thorough examination of the relevant literature, incorporating recent breakthroughs in the field. The methodology section succinctly outlines the approach, with a specific focus on CTC analysis and its implications for BC diagnosis and prognosis. Through this review, insights into the dynamic interplay between the immune system and BC are highlighted, with a specific emphasis on the role of CTCs in advancing diagnostic methodologies and refining prognostic assessments. Furthermore, this review presents objective and substantiated results, contributing to a deeper understanding of the immunological complexity in BC. In conclusion, this investigation underscores the significance of exploring the immunological profile of BC patients, providing valuable insights into novel advances in diagnosis and prognosis through the utilization of CTCs. The objective presentation of findings emphasizes the crucial role of the immune system in BC dynamics, thereby opening avenues for enhanced clinical management strategies.

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“…CTM that are enriched with platelets have been shown to confer enhanced survival and metastatic advantages compared to individual CTCs and hold immense promise as target entities for various clinical interventions aimed at improving patient outcomes. 18 There is a great need for effective and specific CTM isolation techniques to better clarify the mechanisms underlying the function of different CTM subtypes in tumor metastasis. Microfluidic chip technology possesses obvious advantages in its ability to isolate unique cell populations in a rapid and efficient manner.…”

Section: Introductionmentioning

confidence: 99%

Hypoxia stimulates CTC-platelet cluster formation to promote breast cancer metastasis

Zhou,

Zhu,

Shen

et al. 2024

iScience

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Circulating tumour cells: The Good, the Bad and the Ugly

Cited by 18 publications

References 134 publications

Circulating tumor cells shielded with extracellular vesicle-derived CD45 evade T cell attack to enable metastasis

Circulating tumor cells shielded with extracellular vesicle-derived CD45 evade T cell attack to enable metastasis

Exploring the Immunological Profile in Breast Cancer: Recent Advances in Diagnosis and Prognosis through Circulating Tumor Cells

Hypoxia stimulates CTC-platelet cluster formation to promote breast cancer metastasis

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