Circulating zonulin levels in newly diagnosed Chinese type 2 diabetes patients

Zhang, D.; Zhang, L.; Zheng, Yujiao; Yue, Fangzhi; Russell, Ryan; Zeng, Yue

doi:10.1016/j.diabres.2014.08.017

Cited by 79 publications

(98 citation statements)

References 25 publications

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“…Increased serum zonulin levels, by weakening the cytoskeletal structure between intestinal cells, are suggested to lead to IR and to also affect the other aspects of the metabolic syndrome in obese individuals through an increase in mucosal absorption surface (5). Studies investigating the relationship between zonulin, which has an effect on increased intestinal permeability, and IR, have yielded varying results (3,5,27).…”

Section: Discussionmentioning

confidence: 99%

“…Circulating zonulin levels are considered to be a useful marker of intestinal permeability. Recently, higher circulating zonulin levels were reported in obese adult subjects as compared to the non-obese and also in adults with glucose intolerance as compared to those with normal glucose tolerance (3,5). Significantly higher serum zonulin levels were also reported in obese subjects with biopsy-confirmed nonalcoholic fatty liver disease (NAFLD) than in obese subjects without NAFLD (4).…”

Section: Introductionmentioning

confidence: 99%

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The Relationship between Serum Zonulin Level and Clinical and Laboratory Parameters of Childhood Obesity

Küme¹,

Acar²,

Tuhan³

et al. 2017

Jcrpe

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Objective:To investigate the relationship between zonulin levels and clinical and laboratory parameters of childhood obesity.Methods:The study included obese children with a body mass index (BMI) >95th percentile and healthy children who were of similar age and gender distribution. Clinical (BMI, waist circumferences, mid-arm circumference, triceps skinfold, percentage of body fat, systolic blood pressure, diastolic blood pressure) and biochemical (glucose, insulin, lipid levels, thyroid function tests, cortisol, zonulin and leptin levels) parameters were measured.Results:A total of 43 obese subjects (23 males, mean age: 11.1±3.1 years) and 37 healthy subjects (18 males, mean age: 11.5±3.5 years) were included in this study. Obese children had significantly higher insulin, homeostasis model assessment of insulin resistance, triglyceride, total cholesterol, low-density lipoprotein cholesterol, high-density lipoprotein cholesterol (HDL-C), zonulin and leptin levels than healthy children (p<0.05), while glucose levels were not different (p>0.05). Comparison of the obese children with and without insulin resistance showed no statistically significant differences for zonulin levels (p>0.05). Zonulin levels were found to negatively correlate with HDL-C and positively correlate with leptin levels, after adjusting for age and BMI.Conclusion:To the best of our knowledge, this is the first study investigating the relationship between circulating zonulin level (as a marker of intestinal permeability) and insulin resistance and leptin (as markers of metabolic disturbances associated with obesity) in childhood obesity. The results showed that zonulin was significantly higher in obese children when compared to healthy children, a finding indicating a potential role of zonulin in the etiopathogenesis of obesity and related disturbances.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Relationship between Serum Zonulin Level and Clinical and Laboratory Parameters of Childhood Obesity

Küme¹,

Acar²,

Tuhan³

et al. 2017

Jcrpe

View full text Add to dashboard Cite

show abstract

“…Third, we only included women in our study. To our best knowledge, there are no differences of serum zonulin between men and women [2, 60]. However, more and larger studies are needed to investigate whether our study results are transferable to men.…”

Section: Discussionmentioning

confidence: 88%

Gut microbiota, dietary intakes and intestinal permeability reflected by serum zonulin in women

et al. 2018

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PurposeIncreased gut permeability causes the trespass of antigens into the blood stream which leads to inflammation. Gut permeability reflected by serum zonulin and diversity of the gut microbiome were investigated in this cross-sectional study involving female study participants with different activity and BMI levels.Methods102 women were included (BMI range 13.24–46.89 kg m−2): Anorexia nervosa patients (n = 17), athletes (n = 20), normal weight (n = 25), overweight (n = 21) and obese women (n = 19). DNA was extracted from stool samples and subjected to 16S rRNA gene analysis (V1–V2). Quantitative Insights Into Microbial Ecology (QIIME) was used to analyze data. Zonulin was measured with ELISA. Nutrient intake was assessed by repeated 24-h dietary recalls. We used the median of serum zonulin concentration to divide our participants into a “high-zonulin” (> 53.64 ng/ml) and “low-zonulin” (< 53.64 ng/ml) group.ResultsThe alpha-diversity (Shannon Index, Simpson Index, equitability) and beta-diversity (unweighted and weighted UniFrac distances) of the gut microbiome were not significantly different between the groups. Zonulin concentrations correlated significantly with total calorie-, protein-, carbohydrate-, sodium- and vitamin B12 intake. Linear discriminant analysis effect size (LEfSe) identified Ruminococcaceae (LDA = 4.163, p = 0.003) and Faecalibacterium (LDA = 4.151, p = 0.0002) as significantly more abundant in the low zonulin group.ConclusionButyrate-producing gut bacteria such as Faecalibacteria could decrease gut permeability and lower inflammation. The diversity of the gut microbiota in women does not seem to be correlated with the serum zonulin concentration. Further interventional studies are needed to investigate gut mucosal permeability and the gut microbiome in the context of dietary factors.Electronic supplementary materialThe online version of this article (10.1007/s00394-018-1784-0) contains supplementary material, which is available to authorized users.

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“…When measured from circulation, zonulin has been linked with insulin resistance (Moreno-Navarrete et al, 2012), type 2 diabetes (Zhang et al, 2014), higher BMI (Moreno-Navarrete et al, 2012) and non-alcoholic fatty liver disease (Pacifico et al, 2014). The link between circulating zonulin and intestinal zonulin remains somewhat elusive, since circulating zonulin might not originate only from the gut (Wang et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

Probiotic With or Without Fiber Controls Body Fat Mass, Associated With Serum Zonulin, in Overweight and Obese Adults—Randomized Controlled Trial

et al. 2016

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BackgroundThe gut microbiota is interlinked with obesity, but direct evidence of effects of its modulation on body fat mass is still scarce. We investigated the possible effects of Bifidobacterium animalisssp. lactis 420 (B420) and the dietary fiber Litesse® Ultra polydextrose (LU) on body fat mass and other obesity-related parameters.Methods225 healthy volunteers (healthy, BMI 28–34.9) were randomized into four groups (1:1:1:1), using a computer-generated sequence, for 6 months of double-blind, parallel treatment: 1) Placebo, microcrystalline cellulose, 12 g/d; 2) LU, 12 g/d; 3) B420, 1010 CFU/d in microcrystalline cellulose, 12 g/d; 4) LU + B420, 12 g + 1010 CFU/d. Body composition was monitored with dual-energy X-ray absorptiometry, and the primary outcome was relative change in body fat mass, comparing treatment groups to Placebo. Other outcomes included anthropometric measurements, food intake and blood and fecal biomarkers. The study was registered in Clinicaltrials.gov (NCT01978691).FindingsThere were marked differences in the results of the Intention-To-Treat (ITT; n = 209) and Per Protocol (PP; n = 134) study populations. The PP analysis included only those participants who completed the intervention with > 80% product compliance and no antibiotic use. In addition, three participants were excluded from DXA analyses for PP due to a long delay between the end of intervention and the last DXA measurement. There were no significant differences between groups in body fat mass in the ITT population. However, LU + B420 and B420 seemed to improve weight management in the PP population. For relative change in body fat mass, LU + B420 showed a − 4.5% (− 1.4 kg, P = 0.02, N = 37) difference to the Placebo group, whereas LU (+ 0.3%, P = 1.00, N = 35) and B420 (− 3.0%, P = 0.28, N = 24) alone had no effect (overall ANOVA P = 0.095, Placebo N = 35). A post-hoc factorial analysis was significant for B420 (− 4.0%, P = 0.002 vs. Placebo). Changes in fat mass were most pronounced in the abdominal region, and were reflected by similar changes in waist circumference. B420 and LU + B420 also significantly reduced energy intake compared to Placebo. Changes in blood zonulin levels and hsCRP were associated with corresponding changes in trunk fat mass in the LU + B420 group and in the overall population. There were no differences between groups in the incidence of adverse events.DiscussionThis clinical trial demonstrates that a probiotic product with or without dietary fiber controls body fat mass. B420 and LU + B420 also reduced waist circumference and food intake, whereas LU alone had no effect on the measured outcomes.

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Circulating zonulin levels in newly diagnosed Chinese type 2 diabetes patients

Cited by 79 publications

References 25 publications

The Relationship between Serum Zonulin Level and Clinical and Laboratory Parameters of Childhood Obesity

The Relationship between Serum Zonulin Level and Clinical and Laboratory Parameters of Childhood Obesity

Gut microbiota, dietary intakes and intestinal permeability reflected by serum zonulin in women

Probiotic With or Without Fiber Controls Body Fat Mass, Associated With Serum Zonulin, in Overweight and Obese Adults—Randomized Controlled Trial

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