Circulation and in vivo distribution of duck hepatitis A virus types 1 and 3 in infected ducklings

Lin, Shaoli; Cong, Richao; Zhang, Ruihua; Chen, Junhao; Xia, Ling; Xie, Zhixun; Wang, Yu; Zhu, Yanli; Jiang, Shijin

doi:10.1007/s00705-015-2648-z

Cited by 49 publications

(38 citation statements)

References 26 publications

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“…DHAV‐3 accounts for an increasing proportion of pathogens in South Korea (Cha, Roh, Kang, Kim, & Jang, ; Kim et al., ), mainland China (Liu et al., ) and Vietnam (Roan et al., ). Additionally, outbreaks with co‐infections of DHAV‐1 and DHAV‐3 have recently been reported in mainland China and South Korea (Lin et al., ; Soliman et al., ). Beginning in 2013, two officially approved live‐attenuated vaccines against DHAV‐1 strains (CH60 and A66) have been widely used to vaccinate breeder ducks in China; however, investigations into the epidemiology of DHAV infection have lacked since that time.…”

Section: Introductionmentioning

confidence: 99%

Molecular epidemiology of duck hepatitis a virus types 1 and 3 in China, 2010-2015

Wen

Zhu

Cheng

et al. 2017

Transbound Emerg Dis

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Duck hepatitis A virus (DHAV) is the most common aetiologic agent of duck virus hepatitis (DVH), causing substantial economic losses in the duck industry worldwide. In China, officially approved DHAV-1 live-attenuated vaccines have been used widely to vaccinate breeder ducks since 2013. However, following the reports of DVH outbreaks, it has become necessary to assess the epidemiological situation of this virus in China. We conducted molecular epidemiological analyses of 32 DHAV field isolates while analysing the samples from ducks suspected of having hepatitis collected from commercial duck farms in China between May 2010 and December 2015. Considerable changes were observed in the epidemiology of DHAV-1 and DHAV-3 in China over time. A higher number of DHAV-1 strains were isolated during 2010-2012, coinciding with the widespread use of officially approved DHAV-1 live vaccine strains beginning in 2013. In contrast, a higher rate of DHAV-3 causing DHAV infections was observed between 2013 and 2015. Phylogenetic analyses based on the full-length VP1 gene were performed on these field isolates and using reference strains available in GenBank. DHAV-1 field isolates were evaluated in two groups: one group closely related to prototype strains and circulating in China between 2010 and 2012 and another group exhibiting genetic and serological differences from prototype strains. All DHAV-3 strains isolated in this study were grouped as monophyletic, which has become the predominant viral type, particularly in Shandong and Sichuan provinces, since 2013. In conclusion, these data provide updated information on the genetic and serological diversity of DHAV-1 and DHAV-3, and our findings may serve as a foundation for the prevention of, and vaccine development for, DHAV in China.

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Section: Introductionmentioning

confidence: 99%

Molecular epidemiology of duck hepatitis a virus types 1 and 3 in China, 2010-2015

Wen

Zhu

Cheng

et al. 2017

Transbound Emerg Dis

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“…Given that DHAV-3 is most prevalent in duck industry in East and South Asia (Fu et al, 2008; Soliman et al, 2015; Doan et al, 2016; Lin et al, 2016), the resistance breeding against DHAV-3 was started in 2014 in our laboratory by using 88 families of a Pekin duck specialized line (Z8) as a tested flock (Zhang et al, 2016). As a first step toward the resistance breeding, the authors investigated the resistance and susceptibility of Pekin duck strain Z8, a lean-type line of Pekin duck, to DHAV-3 by using experimental infections.…”

Section: Introductionmentioning

confidence: 99%

Host Differences Affecting Resistance and Susceptibility of the Second Generation of a Pekin Duck Flock to Duck Hepatitis A Virus Genotype 3

et al. 2017

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Earlier work suggested the possibility to anti duck hepatitis A virus genotype 3 (DHAV-3) using the resistance breeding strategy. Here, we report the creation of the second generations of a resistant Pekin duck flock (designated Z8R2) and a highly susceptible Pekin duck flock (designated Z8S2) and the investigation of their responses to DHAV-3. Experimental infection with DHAV-3 at 7 days of age resulted in a high mortality (66.3%) in 11 susceptible Z8S2 families and an extremely low mortality rate (2.67%) in 32 Z8R2 families, indicating that Z8R2 exhibits strong resistance to DHAV-3, while Z8S2 is highly susceptible to the virus. Detection of DHAV-3 in the liver between 1 and 60 hours post inoculation (hpi) suggests that DHAV-3 can be replicated rapidly and efficiently in the liver of Z8S2, whereas the replication of the virus in the liver of Z8R2 is suppressed greatly. High levels of serum biochemical markers (e.g., ALT, AST, ALP and GGT) were detected in Z8S2 at 24 hpi, which were significantly higher than those in Z8R2. Analysis of transcripts in the liver revealed that the expression levels of several pattern recognition receptors (PRRs) (e.g., TLR4/7, RIG-1 and MDA5) and cytokines (e.g., IL-2, IL-6, IL-8, IFN-α, and IFN-γ) in Z8S2 were significantly higher than those in Z8R2 at 12 and 24 hpi. Together these findings suggest that Z8R2 and Z8S2 Pekin ducks, which were derived from the same Z8 line, exhibit disparate pathogenic outcomes following DHAV-3 infection. Therefore, it is possible to select a Pekin duck flock resistant to DHAV-3 employing the strategy described here. It is likely that the high viral load and the strong inflammatory response correlate with the high susceptibility of Z8S2 Pekin ducks to DHAV-3.

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“…Our previous research had revealed that duck hepatitis A virus (DHAV) type 1 (DHAV-1) and type 3 (DHAV-3) were detected with high viral loads in all the tested organs (liver, spleen, thymus, pancreas, brain, thymus, kidney and BF) of clinical infected Cherry Valley ducklings [44]. In this study, high viral loads of DAstV-1 was firstly identified in all the different tested organs (liver, spleen, kidney, thymus, BF, heart and small intestines) of the infected ducklings, indicating that DAstV-1 also had extensive tissue tropism.…”

Section: Discussionmentioning

confidence: 99%

A novel method to rescue and culture duck Astrovirus type 1 in vitro

Zhang

Lan

et al. 2019

Virol J

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Background Reverse genetics systems enable the manipulation of viral genomes and therefore serve as robust reverse genetic tools to study RNA viruses. A DNA-launched rescue system initiates the transcription of viral genomic cDNA from eukaryotic promoter in transfected cells, generating homogenous RNA transcripts in vitro and thus enhancing virus rescue efficiency. As one of the hazardous pathogens to ducklings, the current knowledge of the pathogenesis of duck astrovirus type 1 (DAstV-1) is limited. The construction of a DNA-launched rescue system can help to accelerate the study of the virus pathogenesis. However, there is no report of such a system for DAstV-1. Methods In this study, a DNA-launched infectious clone of DAstV-1 was constructed from a cDNA plasmid, which contains a viral cDNA sequence flanked by hammerhead ribozyme (HamRz) and a hepatitis delta virus ribozyme (HdvRz) sequence at both terminals of the viral genome. A silent nucleotide mutation creating a Bgl II site in the ORF2 gene was made to distinguish the rescued virus (rDAstV-1) from the parental virus (pDAstV-1). Immunofluorescence assay (IFA) and western blot were conducted for rescued virus identification in duck embryo fibroblast (DEF) cells pre-treated with trypsin. The growth characteristics of rDAstV-1 and pDAstV-1 in DEF cells and the tissue tropism in 2-day-old ducklings of rDAstV-1 and pDAstV-1 were determined. Results The infectious DAstV-1 was successfully rescued from baby hamster kidney (BHK-21) cells and could propagate in DEF cells pre-treated with 1 μg/ml trypsin. Upon infection of DEF cells pre-treated with trypsin, DAstV-1 mRNA copies were identified after serial passaging, and the result showed that rDAstV-1 and pDAstV-1 shared similar replication kinetics. Animal experiment showed that the rDAstV-1 had an extensive tissue tropism, and the virus was capable of invading both the central and the peripheral immune organs in infected ducklings. Conclusions An improved DNA-launched reverse genetics system for DAstV-1 was firstly constructed. Infectious virus recovered from BHK-21 cells could propagate in DEF cells pre-treated with trypsin. This is the first report of the successful in vitro cultivation of DAstV-1. We believe this valuable experimental system will contribute to the further study of DAstV-1 genome function and pathogenesis.

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Circulation and in vivo distribution of duck hepatitis A virus types 1 and 3 in infected ducklings

Cited by 49 publications

References 26 publications

Molecular epidemiology of duck hepatitis a virus types 1 and 3 in China, 2010-2015

Molecular epidemiology of duck hepatitis a virus types 1 and 3 in China, 2010-2015

Host Differences Affecting Resistance and Susceptibility of the Second Generation of a Pekin Duck Flock to Duck Hepatitis A Virus Genotype 3

A novel method to rescue and culture duck Astrovirus type 1 in vitro

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