circUSP34 accelerates osteosarcoma malignant progression by sponging miR‐16‐5p

Lou, Jingbing; Zhang, Hongliang; Xu, Jiuhui; Ren, Tingting; Huang, Yi; Tang, Xiaodong; Guo, Wei

doi:10.1111/cas.15147

Cited by 13 publications

(10 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To date, more and more circRNAs have been discovered to be related to pathophysiological events in OS. 17 , 19 , 20 For example, circDOCK1 promotes the tumorigenesis of OS through the miR‐339‐3p/IGF1R axis. 21 However, it remains unclear whether circUSP48 exerts its functional role in OS.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Silencing circUSP48 suppresses osteosarcoma progression by regulating the miR‐335/ smad nuclear interacting protein 1 pathway

Luo

Yang

Xiao-pin

et al. 2023

Clinical Laboratory Analysis

View full text Add to dashboard Cite

Background Circular RNAs (circRNAs) can have a critical function in the multi‐processes of osteosarcoma (OS). Nevertheless, whether circUSP48 is involved in OS progression remains unclear. Methods In the current work, the expression of circUSP48, miR‐335 and SNIP1 in OS cell lines and tissues were evaluated using qRT‐PCR. Then, Sanger sequencing, RNase R treatment and FISH assay were performed for circUSP48 validation. Furthermore, the function and potential mechanisms of circUSP48 in OS were investigated by performing loss‐of‐function experiments. Results Silencing circUSP48 could suppress proliferation, invasion as well as migration of OS cells in vitro, also inhibiting the growth of tumor in vivo. Importantly, circUSP48 promoted OS malignancy by sponging miR‐335 to upregulate SNIP1. Conclusion Overall, these findings suggested that circUSP48 acted as an oncogene in OS, which might become a new target for OS therapy.

show abstract

Section: Discussionmentioning

confidence: 99%

“…After injection, we monitored tumor volumes every 5 days. 4 weeks later, the tumors were weighed and stored for qRT‐PCR and immunohistochemical assay 17 …”

Section: Methodsmentioning

confidence: 99%

Silencing circUSP48 suppresses osteosarcoma progression by regulating the miR‐335/ smad nuclear interacting protein 1 pathway

Luo

Yang

Xiao-pin

et al. 2023

Clinical Laboratory Analysis

View full text Add to dashboard Cite

show abstract

“…For instance, circUBE2J2 reduced migration and proliferation of hepatocellular carcinoma cells via controlling miR-370-5p [ 26 ]. CircRNA ubiquitin-specific protease 34 (circUSP34) exacerbated cell malignant behaviors of osteosarcoma by inhibiting miR-16-5p [ 27 ]. CircRNA-09505 enhanced inflammatory reaction in rheumatoid arthritis through absorbing miR-6089 [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

Circ_0005526 contributes to interleukin-1β-induced chondrocyte injury in osteoarthritis via upregulating transcription factor 4 by interacting with miR-142-5p

Wahafu

Tuo

et al. 2022

Bioengineered

View full text Add to dashboard Cite

Circular RNAs (circRNAs) can regulate the progression of osteoarthritis (OA) via serving as competing endogenous RNAs (ceRNAs). This work was performed for functional research of circ_0005526 in Interleukin-1β (IL-1β)-induced OA injury. Circ_0005526, microRNA-142-5p (miR-142-5p) or transcription factor 4 (TCF4) expression was measured via reverse transcription-quantitative polymerase chain reaction assay. Cell analysis was performed by Cell Counting Kit-8 assay for cell viability, EdU assay for cell proliferation and flow cytometry for cell apoptosis. The protein level detection was conducted using western blot. Target analysis was carried out via dual-luciferase reporter assay and RNA pull-down assay. Circ_0005526 was upregulated in OA cartilage tissues and IL-1β-exposed chondrocyte cells. IL-1β inhibited cell viability and proliferation but enhanced cell apoptosis and inflammation, then these damages were attenuated after downregulation of circ_0005526. Circ_0005526 interacted with miR-142-5p, and circ_0005526 knockdown suppressed IL-1β-induced OA progression through upregulating miR-142-5p. TCF4 was regulated by circ_0005526 via targeting miR-142-5p. The function of circ_0005526 was also achieved by upregulation of TCF4. These results unraveled that circ_0005526 promoted IL-1β-induced chondrocyte injury in OA via suppressing miR-142-5p binding to TCF4.

show abstract

“…Several circRNAs have been reported to play an oncogenic role in OS progression through malignant phenotype promotion, including circOMA1, circUSP34, and circNT5C2 [ 12 , 28 , 29 ]. In this study, circDOCK1, a novel circRNA, was chosen by screening the GSE140256 dataset.…”

Section: Discussionmentioning

confidence: 99%

CircDOCK1 Regulates miR-186/DNMT3A to Promote Osteosarcoma Progression

Jin¹,

Jia²,

Chen³

et al. 2022

Biomedicines

View full text Add to dashboard Cite

Background: Circular RNAs (circRNAs), as a class of endogenous RNAs, are implicated in osteosarcoma (OS) progression. However, the functional properties of circDOCK1 in OS have been largely unexplored. The present study demonstrated the regulatory mechanism of circDOCK1 in OS. Methods: QRT-PCR and Western blots were used to determine the abundances of circDOCK1, miR-186, and DNMT3A. Cell counting kit-8 (CCK-8), 5-Ethynyl-2′-deoxyuridine (EdU), colony formation, Transwell, and wound healing assays were used to examine cellular multiplication, motility, and invasion. Luciferase reporter analysis, RNA immunoprecipitation (RIP), and pull-down assays were used to verify target relationships. Xenograft models were used to analyze in vivo function. Results: OS tissues and cells showed high levels of circDOCK1. By knocking down circDOCK1, cellular multiplication, motility, and invasion were suppressed. Furthermore, silencing circDOCK1 suppressed the growth of tumor xenografts. According to mechanistic studies, miR-186 targets DNA methyltransferases 3A (DNMT3A) directly and acts as a circDOCK1 target. Furthermore, circDOCK1 upregulated DNMT3A expression through sponging miR-186 to regulate the progression of OS. Conclusions: CircDOCK1 promotes OS progression by interacting with miR-186/DNMT3ADNMT3A, representing a novel therapeutic approach.

show abstract

circUSP34 accelerates osteosarcoma malignant progression by sponging miR‐16‐5p

Abstract: This is an open access article under the terms of the Creat ive Commo ns Attri butio n-NonCo mmerc ial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

Cited by 13 publications

References 49 publications

Silencing circUSP48 suppresses osteosarcoma progression by regulating the miR‐335/ smad nuclear interacting protein 1 pathway

Silencing circUSP48 suppresses osteosarcoma progression by regulating the miR‐335/ smad nuclear interacting protein 1 pathway

Circ_0005526 contributes to interleukin-1β-induced chondrocyte injury in osteoarthritis via upregulating transcription factor 4 by interacting with miR-142-5p

CircDOCK1 Regulates miR-186/DNMT3A to Promote Osteosarcoma Progression

Contact Info

Product

Resources

About