Cis-Regulatory Elements Determine Germline Specificity and Expression Level of an Isopentenyltransferase Gene in Sperm Cells of Arabidopsis

Zhang, Jinghua; Yuan, Tong; Duan, Xiaomeng; Wei, Xiaoping; Shi, Tao; Li, Jia; Russell, Scott D.; Gou, Xiaoping

doi:10.1104/pp.15.01510

Cited by 8 publications

(10 citation statements)

References 47 publications

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“…3A). The full-length CaMV 35S promoter and the minimal CaMV 35S promoter are not active in Arabidopsis pollen (Wilkinson et al, 1997;Zhang et al, 2016). Sperm-cell-specific expression was observed in mature pollen in 10 out of 10 transgenic lines, indicating that this region of DNA was sufficient to activate germline transcription (Fig.…”

Section: A Small Region Of the Duo1 Promoter Is Sufficient For Germlimentioning

confidence: 99%

“…Other germline CREs, which direct sperm-cell-specific expression, were identified in the promoter of the PzIPT1 gene (Zhang et al, 2016). Disruption of two 6-bp Male Gamete Selective Activation (MGSA) motifs located near the transcription start site inactivated PzIPT1 promoter activity in sperm.…”

Section: Relationship With Other Known Male Germline-cresmentioning

confidence: 99%

“…In this model, the binding of the regulatory factor GRSF to a silencer element represses expression in nonmale germline cells, such that transcriptional activation in germ cells results from derepression. However, mutation of the putative GRSF binding site in the DUO1 promoter did not alter germ cell specificity , and the PzIPT1 promoter does not contain the GRSF binding site (Zhang et al, 2016). Further, the activity profiles of the DUO1 (Fig.…”

Section: Relationship With Other Known Male Germline-cresmentioning

confidence: 99%

“…MGSA motifs were found in other spermcell-expressed genes and shown to be required for the activity of six sperm-cell-expressed genes in Arabidopsis. However, unlike ROD1 these MGSA motifs activate transcription only at late stages of pollen development (Zhang et al, 2016). Therefore, they are unlikely to play critical roles in establishing male germline fate but could promote the expression of genes required for sperm cell maturation through responses to the plant hormone cytokinin (Zhang et al, 2016).…”

Section: Relationship With Other Known Male Germline-cresmentioning

confidence: 99%

“…More recently, a CRE that directs sperm-cell-specific expression in Arabidopsis was identified in the promoter of the Plumbago zeylanica isopentenyltransferase (PzIPT1) gene (Zhang et al, 2016). This study identified a regulatory region consisting of duplicated 6-bp Male Gamete Selective Activation (MGSA) motifs, located near the transcription start site.…”

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confidence: 99%

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A Conserved cis-Regulatory Module Determines Germline Fate through Activation of the Transcription Factor DUO1 Promoter

et al. 2016

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The development of the male germline within pollen relies upon the activation of numerous target genes by the transcription factor DUO POLLEN1 (DUO1). The expression of DUO1 is restricted to the male germline and is first detected shortly after the asymmetric division that segregates the germ cell lineage. Transcriptional regulation is critical in controlling DUO1 expression, since transcriptional and translational fusions show similar expression patterns. Here, we identify key promoter sequences required for the germline-specific regulation of DUO1 transcription. Combining promoter deletion analyses with phylogenetic footprinting in eudicots and in Arabidopsis accessions, we identify a cis-regulatory module, Regulatory region of DUO1 (ROD1), which replicates the expression pattern of DUO1 in Arabidopsis (Arabidopsis thaliana). We show that ROD1 from the legume Medicago truncatula directs male germline-specific expression in Arabidopsis, demonstrating conservation of DUO1 regulation among eudicots. ROD1 contains several short conserved cis-regulatory elements, including three copies of the motif DNGTGGV, required for germline expression and tandem repeats of the motif YAACYGY, which enhance DUO1 transcription in a positive feedback loop. We conclude that a cis-regulatory module conserved in eudicots directs the spatial and temporal expression of the transcription factor DUO1 to specify male germline fate and sperm cell differentiation.

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Section: A Small Region Of the Duo1 Promoter Is Sufficient For Germlimentioning

confidence: 99%

Section: Relationship With Other Known Male Germline-cresmentioning

confidence: 99%

Section: Relationship With Other Known Male Germline-cresmentioning

confidence: 99%

Section: Relationship With Other Known Male Germline-cresmentioning

confidence: 99%

mentioning

confidence: 99%

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A Conserved cis-Regulatory Module Determines Germline Fate through Activation of the Transcription Factor DUO1 Promoter

et al. 2016

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Identification of Cis-Regulatory Modules that Function in the Male Germline of Flowering Plants

Peters

Aidley

Cadzow

et al. 2017

Methods in Molecular Biology

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The male germline of flowering plants develops within the vegetative cell of the male gametophyte and displays a distinct transcriptional profile. Key to understanding the development of this unique cell lineage is determining how gene expression is regulated within germline cells. This knowledge impacts upon our understanding of cell specification, differentiation, and plant fertility. Here, we describe methods to identify cis-regulatory modules (CRMs) that act as key regulatory regions in the promoters of germline-expressed genes. We detail the complimentary techniques of phylogenetic footprinting and the use of fluorescent reporters in pollen for the identification and verification of CRMs.

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Cytokinin regulates female gametophyte development by cell cycle modulation in Arabidopsis thaliana

et al. 2022

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Cis-Regulatory Elements Determine Germline Specificity and Expression Level of an Isopentenyltransferase Gene in Sperm Cells of Arabidopsis

Cited by 8 publications

References 47 publications

A Conserved cis-Regulatory Module Determines Germline Fate through Activation of the Transcription Factor DUO1 Promoter

A Conserved cis-Regulatory Module Determines Germline Fate through Activation of the Transcription Factor DUO1 Promoter

Identification of Cis-Regulatory Modules that Function in the Male Germline of Flowering Plants

Cytokinin regulates female gametophyte development by cell cycle modulation in Arabidopsis thaliana

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