Cited2 Gene Controls Pluripotency and Cardiomyocyte Differentiation of Murine Embryonic Stem Cells through Oct4 Gene

Li, Qiang; Ramírez-Bergeron, Diana L.; Dunwoodie, Sally L.; Yang, Yu Chung

doi:10.1074/jbc.m112.378034

Cited by 25 publications

(36 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In contrast, the expression of Oct4 remained high in differentiating Cited2 ⌬/Ϫ ESCs (Fig. 6A), which is consistent with our previous report that Cited2 regulates expression of the Oct4 gene to control ESC differentiation during normoxia (22). Fgf5, a primitive epiblast marker for ESC differentiation, was induced in WT EBs at day 3.5 and the expression level was gradually reduced at EB days 4.5 and 5.5.…”

Section: As Did In Undifferentiated Cited2supporting

confidence: 81%

“…Cited2 was recently characterized as a pro-differentiation factor in the mouse ESCs (22,23). In this study, we report that mESCs with a deletion in the gene for Cited2 (Cited2 ⌬/Ϫ ) have swollen mitochondria, increased mitochondrial number, and reduced mitochondrial function as reflected by a reduced rate of glucose oxidation.…”

Section: Crebmentioning

confidence: 81%

“…During ESCs differentiating to cardiomyocytes, the rate of lactate production, normalized by time and total protein mass was reported to be reduced to 2-fold, supporting the notion that metabolic reprogramming is essential for proper ESC differentiation (19). We previously demonstrated that Cited2 ⌬/Ϫ ESCs exhibit defective cardiomyocyte and hematopoietic differentiation during normoxia (22). In WT and Cited2 ⌬/Ϫ EBs favoring cardiomyocyte differentiation, glucose oxidation was reduced by 30% in Cited2 ⌬/Ϫ EBs compared with the WT control (Fig.…”

Section: Increased Glycolytic Activity and Impaired Hypoxic Differentmentioning

confidence: 89%

“…Because Cited2 deletion did not affect pluripotency in undifferentiated ESCs from our previous study (22), we tested whether enhanced glycolysis in Cited2…”

Section: Enhanced Aerobic Glycolysis and Reduced Glucose Oxidation Inmentioning

confidence: 99%

“…ESCs were periodically selected with puromycin to maintain a population of undifferentiated ESC. ESCs were induced to differentiate via a methylcellulose-based method as described previously (22). For hypoxia experiments, ESCs were cultured or induced to differentiate in an incubator with an adjustable oxygen concentration, as described elsewhere (24).…”

Section: ⌬/ϫmentioning

confidence: 99%

See 4 more Smart Citations

Cited2, a Transcriptional Modulator Protein, Regulates Metabolism in Murine Embryonic Stem Cells

Hakimi

Liu

et al. 2014

Journal of Biological Chemistry

Self Cite

View full text Add to dashboard Cite

Background:The function of HIF-1, a master regulator of metabolism, is in part modulated by Cited2. The role of Cited2 in murine embryonic stem cell (mESC) glucose metabolism remains unknown. Results: Deletion of Cited2 in mESCs results in impaired mitochondria morphology, reduced glucose oxidation, increased glycolysis, and defective mESC differentiation. Conclusion: Cited2 coordinates glucose metabolism to regulate mESC differentiation. Significance: Cited2 is a potential target for metabolic reprogramming in mESCs.

show abstract

Section: As Did In Undifferentiated Cited2supporting

confidence: 81%

Section: Crebmentioning

confidence: 81%

Section: Increased Glycolytic Activity and Impaired Hypoxic Differentmentioning

confidence: 89%

“…Because Cited2 deletion did not affect pluripotency in undifferentiated ESCs from our previous study (22), we tested whether enhanced glycolysis in Cited2…”

Section: Enhanced Aerobic Glycolysis and Reduced Glucose Oxidation Inmentioning

confidence: 99%

Section: ⌬/ϫmentioning

confidence: 99%

See 3 more Smart Citations

Cited2, a Transcriptional Modulator Protein, Regulates Metabolism in Murine Embryonic Stem Cells

Hakimi

Liu

et al. 2014

Journal of Biological Chemistry

Self Cite

View full text Add to dashboard Cite

show abstract

Hepatitis C virus‐induced differential transcriptional traits in host cells after persistent infection elimination by direct‐acting antivirals in cell culture

Castro,

Calvo,

Oliveros

et al. 2024

Journal of Medical Virology

View full text Add to dashboard Cite

Chronic hepatitis C virus infection (HCV) causes liver inflammation and fibrosis, leading to the development of severe liver disease, such as cirrhosis or hepatocellular carcinoma (HCC). Approval of direct‐acting antiviral drug combinations has revolutionized chronic HCV therapy, with virus eradication in >98% of the treated patients. The efficacy of these treatments is such that it is formally possible for cured patients to carry formerly infected cells that display irreversible transcriptional alterations directly caused by chronic HCV Infection. Combining differential transcriptomes from two different persistent infection models, we observed a major reversion of infection‐related transcripts after complete infection elimination. However, a small number of transcripts were abnormally expressed in formerly infected cells. Comparison of the results obtained in proliferating and growth‐arrested cell culture models suggest that permanent transcriptional alterations may be established by several mechanisms. Interestingly, some of these alterations were also observed in the liver biopsies of virologically cured patients. Overall, our data suggest a direct and permanent impact of persistent HCV infection on the host cell transcriptome even after virus elimination, possibly contributing to the development of HCC.

show abstract

Hypoxia induces re-entry of committed cells into pluripotency

et al. 2013

View full text Add to dashboard Cite

Adult stem cells reside in hypoxic niches and embryonic stem cells (ESCs) are derived from a low oxygen environment. However, it is not clear whether hypoxia is critical for stem cell fate since for example human ESCs (hESCs) are able to self-renew in atmospheric oxygen concentrations as well. We now show that hypoxia can govern cell fate decision since hypoxia alone can revert hESC- or iPSC-derived differentiated cells back to a stem cell-like state, as evidenced by re-activation of an Oct4-promoter reporter. Hypoxia-induced “de-differentiated” cells also mimic hESCs in their morphology, long-term self-renewal capacity, genome wide mRNA and miRNA profiles, Oct4 promoter methylation state, cell surface markers TRA1-60 and SSEA4 expression and capacity to form teratomas. These data demonstrate that hypoxia can influence cell fate decisions and could elucidate hypoxic niche function.

show abstract

Cited2 Gene Controls Pluripotency and Cardiomyocyte Differentiation of Murine Embryonic Stem Cells through Oct4 Gene

Cited by 25 publications

References 60 publications

Cited2, a Transcriptional Modulator Protein, Regulates Metabolism in Murine Embryonic Stem Cells

Cited2, a Transcriptional Modulator Protein, Regulates Metabolism in Murine Embryonic Stem Cells

Hepatitis C virus‐induced differential transcriptional traits in host cells after persistent infection elimination by direct‐acting antivirals in cell culture

Hypoxia induces re-entry of committed cells into pluripotency

Contact Info

Product

Resources

About