Citral Protects Human Endothelial Cells Against Hydrogen Peroxide-induced Oxidative Stress

Safaeian, Leila; Sajjadi, Seyed Ebrahim; Montazeri, Hossein; Ohadi, Farzaneh; Javanmard, Shaghayegh Haghjoo

doi:10.4274/tjps.galenos.2019.71602

Cited by 10 publications

(4 citation statements)

References 27 publications

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“…Results revealed FRAP IC50 for the compound equal to 1619.6 µM, whereas gallic acid IC50 was equal to 123.1 µM. Interesting results were also obtained by Safaeian et al who decided to determine intra-and extra-cellular FRAP values for citral using a commercial kit [16]. The analysis was performed for 40 min, and the results revealed that pretreatment with citral significantly increased the FRAP value in intracellular fluids at concentrations of 1.25, 2.5, 5, and 10 µg/mL.…”

Section: Discussionmentioning

confidence: 92%

Ability of Selected Monoterpenes to Reduce Fe(III) Ions Being Pro-Neurodegenerative Factors: Tests Based on a FRAP Reaction Extended to 48 Hours

Wojtunik-Kulesza,

Oniszczuk

2024

IJMS

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Monoterpenes are secondary plant metabolites, and such volatile compounds have antioxidant, antibacterial, antiviral, and enzyme inhibitory properties. These compounds are also able to reduce the potentially pro-neurodegenerative trace metal ions that can be sources of free radicals. One basic method used to evaluate the ability of chemical compounds to reduce Fe(III) is FRAP. To date, most studies based on a FRAP assay were performed within several dozen minutes. However, taking into account the diversity of compounds, it is justified to observe their activity over a much longer period of time. The present study aimed to observe the activity of isopulegol, γ-terpinene, α-terpinene, linalool, carvone, citral, and α-phellandrene over a 48 h period. Our study indicates that the lengthened reaction period enhanced activity from several dozen to several hundred percent. The obtained results also revealed an explicit high correlation of the increase in the activity of compounds with the increase in monoterpene concentration. Due to the hydrophobic character of monoterpenes, the FRAP method was modified by the addition of Tween 20. The highest activity was obtained for α-terpinene and γ-terpinene.

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Section: Discussionmentioning

confidence: 92%

Ability of Selected Monoterpenes to Reduce Fe(III) Ions Being Pro-Neurodegenerative Factors: Tests Based on a FRAP Reaction Extended to 48 Hours

Wojtunik-Kulesza,

Oniszczuk

2024

IJMS

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“…This kind of assay did not clearly identify the cause of the effects observed on tubule formation in vitro and seemed to be not completely in line with results obtained in vivo and with some literature data reporting that ARPE-19 cells release pro-angiogenic factors when exposed to oxidative insult, leading in turn to angiogenesis [ 57 ]. On the other hand, indirect effects of H 2 O 2 on HUVECs cells have been reported to depend on the cell model and on the concentration and duration of exposure [ 58 , 59 ].…”

Section: Discussionmentioning

confidence: 99%

Antioxidant Properties of Cerium Oxide Nanoparticles Prevent Retinal Neovascular Alterations In Vitro and In Vivo

et al. 2022

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In this study, we investigated whether cerium oxide nanoparticles (CeO2-NPs), a promising antioxidant nanomaterial, may contrast retinal vascular alterations induced by oxidative damage in vitro and in vivo. For the in vivo experiments, the light damage (LD) animal model of Age-Related Macular Degeneration (AMD) was used and the CeO2-NPs were intravitreally injected. CeO2-NPs significantly decreased vascular endothelial growth factor (VEGF) protein levels, reduced neovascularization in the deep retinal plexus, and inhibited choroidal sprouting into the photoreceptor layer. The in vitro experiments were performed on human retinal pigment epithelial (ARPE-19) cells challenged with H2O2; we demonstrated that CeO2-NPs reverted H2O2-induced oxidative stress-dependent effects on this cell model. We further investigated the RPE–endothelial cells interaction under oxidative stress conditions in the presence or absence of CeO2-NPs through two experimental paradigms: (i) treatment of human umbilical vein endothelial cells (HUVECs) with conditioned media from ARPE-19 cells, and (ii) coculture of ARPE-19 and HUVECs. In both experimental conditions, CeO2-NPs were able to revert the detrimental effect of H2O2 on angiogenesis in vitro by realigning the level of tubule formation to that of the control. Altogether, our results indicate, for the first time, that CeO2-NPs can counteract retinal neovascularization and may be a new therapeutic strategy for the treatment of wet AMD.

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“…These two markers are responsible for the different scents of CO and LO. The phytochemical constituents of both oils possess antioxidant activity, which reduces oxidative stress; citral is one example (Safaeian et al, 2020). Thus, the safety of both oils as antioxidant agents needs to be explored further to support their use.…”

Section: Phytochemical Constituents Of Co and Lomentioning

confidence: 99%

Cytoprotective Properties of Citronella Oil (Cymbopogon nardus (L.) Rendl.) and Lemongrass Oil (Cymbopogon citratus (DC.) Stapf) through Attenuation of Senescent-Induced Chemotherapeutic Agent Doxorubicin on Vero and NIH-3T3 Cells

Salsabila

Wardani

Hasanah

et al. 2023

Asian Pac J Cancer Prev

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Objective: This study aimed to determine the cytoprotective potentials of citronella ( Cymbopogon nardus (L.) Rendl.) essential oil (CO) and lemongrass ( Cymbopogon citratus (DC.) Stapf) essential oil (LO). Methods: The essential oils from citronella and lemongrass were obtained by steam-water distillation, then analyzed using Gas Chromatography-Mass Spectrophotometry (GC-MS) to determine the chemical constituents. The antioxidant activity of CO and LO was compared using a total antioxidant capacity kit. The viability of normal kidney epithelial cells Vero and fibroblast NIH-3T3 as the cell models were tested using a trypan blue exclusion assay. The effect of cellular senescence inhibition on both cell models was measured using senescence-associated β-galactosidase (SA-β-gal) staining. The mechanism of action of CO and LO in the protection of cellular damage against doxorubicin was also confirmed through 2’,7’–dichlorofluorescin diacetate (DCFDA) staining to discover the ability to decrease reactive oxygen species (ROS) levels and a gelatin zymography assay to observe the activity of matrix metalloproteinases (MMPs). Results: The major marker components of CO and LO were citronellal and citral, respectively. Both oils showed low cytotoxic activity against Vero and NIH-3T3 cells, with IC 50 values of over 40 µg/mL. LO exhibited higher antioxidant capacity than CO, but there was no effect on the intracellular ROS level of both oils on Vero and NIH-3T3 cells. However, CO and LO decreased cellular senescence induced by doxorubicin exposure on both cells, as well as suppressed MMP-2 expression. Conclusion: Both CO and LO decrease the cellular senescence and MMP-2 expression with less cytotoxic effects on normal cells independently from their antioxidant capacities. The results were expected to support the use of CO and LO as tissue protective and anti-aging agents in maintaining the body’s cellular health against chemotherapeutics or cellular damaging agents.

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Citral Protects Human Endothelial Cells Against Hydrogen Peroxide-induced Oxidative Stress

Cited by 10 publications

References 27 publications

Ability of Selected Monoterpenes to Reduce Fe(III) Ions Being Pro-Neurodegenerative Factors: Tests Based on a FRAP Reaction Extended to 48 Hours

Ability of Selected Monoterpenes to Reduce Fe(III) Ions Being Pro-Neurodegenerative Factors: Tests Based on a FRAP Reaction Extended to 48 Hours

Antioxidant Properties of Cerium Oxide Nanoparticles Prevent Retinal Neovascular Alterations In Vitro and In Vivo

Cytoprotective Properties of Citronella Oil (Cymbopogon nardus (L.) Rendl.) and Lemongrass Oil (Cymbopogon citratus (DC.) Stapf) through Attenuation of Senescent-Induced Chemotherapeutic Agent Doxorubicin on Vero and NIH-3T3 Cells

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