CLA Reduces Inflammatory Mediators from A427 Human Lung Cancer Cells and A427 Conditioned Medium Promotes Differentiation of C2C12 Murine Muscle Cells

Oraldi, Manuela; Maggiora, Marina; Paiuzzi, Elena; Canuto, Rosa Angela; Muzio, Giuliana

doi:10.1007/s11745-012-3734-6

Cited by 17 publications

(7 citation statements)

References 49 publications

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“…Conventional wisdom holds that tumors release soluble factors that act on distal skeletal muscle tissue to cause atrophy and other metabolic derangements in cancer patients. We evaluated this proposition by testing the effects of murine and human lung cancer/tumor cell CM on C 2 C 12 myotube cultures, a common model to test for cancer/tumor cell-derived atrophic factors (11,37,38,40,46,53,70). Our studies included controls to account for dilution of media and factors released into media by growing nontumorigenic/cachexia-inducing cells and evaluated the effects of timing of CM application relative to the start of differentiation.…”

Section: Discussionmentioning

confidence: 99%

Effects of conditioned media from murine lung cancer cells and human tumor cells on cultured myotubes

Guigni

Velden

Kinsey

et al. 2020

American Journal of Physiology-Endocrinology and Metabolism

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Factors secreted from tumors/tumor cells are hypothesized to cause skeletal muscle wasting in cancer patients. We examined whether cancer cells secrete factors to promote atrophy by evaluating the effects of conditioned media (CM) from murine lung cancer cells and primary cultures of human lung tumor cells on cultured myotubes. We evaluated murine Lewis lung carcinoma (LLC) and KRASG12D cells, and primary cell lines derived from tumor biopsies from patients with lung cancer (hTCM; n = 6). In all experiments, serum content was matched across treatment groups. We hypothesized that CM from murine and human tumor cells would reduce myotube myosin content, decrease mitochondrial content, and increase mitochondrial reactive oxygen species (ROS) production. Treatment of myotubes differentiated for 7 days with CM from LLC and KRASG12D cells did not alter any of these variables. Effects of murine tumor cell CM were observed when myotubes differentiated for 4 days were treated with tumor cell CM and compared with undiluted differentiation media. However, these effects were not apparent if tumor cell CM treatments were compared with control cell CM or dilution controls. Finally, CM from human lung tumor primary cell lines did not modify myosin content or mitochondrial content or ROS production compared with either undiluted differentiated media, control cell CM, or dilution controls. Our results do not support the hypothesis that factors released from cultured lung cancer/tumor cells promote myotube wasting or mitochondrial abnormalities, but we cannot dismiss the possibility that these cells could secrete such factors in vivo within the native tumor microenvironment.

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Section: Discussionmentioning

confidence: 99%

Effects of conditioned media from murine lung cancer cells and human tumor cells on cultured myotubes

Guigni

Velden

Kinsey

et al. 2020

American Journal of Physiology-Endocrinology and Metabolism

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“…Adding human cytokines to murine-derived cell lines could be a limitation of our current study, however, this model is greatly need to study human immune responses to pathogen infections in a small animal model in a systematic and controlled manner [32]. This model was recently used to investigate the effect of the inflammatory mediators secreted from A427 Human Lung [33] serum creatine phosphokinase in choline-deficient humans in C2C12 [34]. Moreover, it is well-established that many protein functions between humans and mice are conserved (similarities between mouse and human genes range from about 70% to 90%) [35,36]; these data therefore set the scene for future larger scale studies to investigate the impact of age and the immune system on muscle cell function in human samples in vitro .…”

Section: Discussionmentioning

confidence: 99%

Activated Lymphocytes Secretome Inhibits Differentiation and Induces Proliferation of C2C12 Myoblasts

Al-Shanti

Durcan²,

Al-Dabbagh³

et al. 2014

Cell Physiol Biochem

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Background/Aims: ageing is associated with a marked decline in immune function which may contribute to the local environment that can influence the regenerative process of skeletal muscle cells. Methods: Herein, we focused on determining the effect of an activated immune system secretome on myoblast differentiation and proliferation as possible means to attenuate adverse effects of muscle aging. C2C12 myoblasts were used as model to assess the impact of lymphocyte conditioned media (CM) following anti-CD3/IL-2 activation. Results: Myoblasts cultured with activated lymphocytes CM exhibited reduced morphological and biochemical differentiation (98±20, p<0.005) and increased entry to the S Phase of the cell cycle (61%±7, p<0.001), when compared with myoblasts cultured with non-activated lymphocytes CM. Associated with increased proliferation and reduced differentiation, muscle specific transcription factors MyoD and myogenin were significantly reduced in C2C12 treated with activated lymphocytes CM vs control CM, respectively (myoD: 0.5±0.12 fold reduction P<0.005); myogenin: 0.38±0.08 fold reduction; p<0.005). Moreover, key protein of proliferation pERK1/2 increased (46±11U/ml, p<0.05) whereas mediator of differentiation pAkt decreased (21±12U/ml, p<0.05) in C2C12 treated with activated vs. non-activated CM. Conclusion: our data demonstrate that, following activation, secretome of the immune system cells elicit marked regulatory effects on skeletal muscle growth and differentiation; enhancing the former with the loss of the latter.

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“…Dentre estes efeitos, destaca-se a sua atividade antitumoral (BEDEL et al, 2017), já observada em diversos tecidos como o pulmão (ORALDI et al, 2013), estomacal (BAE et al, 2013), câncer de endométrio ( WANG et al, 2013), colón (PIERRE et al, 2013), prostático (SONG et al, 2006, sendo que os tumores da glândula mamária parecem ser mais sensíveis a intervenção desse composto (MAJUMDER et al, 2002;KORONOWICZ et al, 2017). Estudos evidenciam que o CLA atua sobre o crescimento e desenvolvimento tumoral em seus diversos estágios (iniciação, promoção, progressão e metástase), por meio da diminuição ou retenção da multiplicação celular, atuando ainda no aumento da morte celular programada (apoptose) e, ainda, sobre a necrose (FIELD; SCHLEY, 2004).…”

Section: Efeitos Benéficos Do Cla Na Saúde Humanaunclassified

Importância Do Ácido Linoleico Conjugado (Cla) Em Produtos De Origem Animal

Lima¹,

Leite²,

Moura³

et al. 2018

Rev. Agro. Amb.

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O termo CLA descreve um conjunto de isômeros geométricos do ácido linoleico com propriedades bioativas distintas, que têm duas duplas ligações separadas por apenas uma ligação simples. Assim, este trabalho trata-se de uma revisão bibliográfica baseada na literatura especializada, fundamentado através de consulta a artigos científicos selecionados em diferentes bases de dados com o objetivo de dar enfoque à importância do ácido linoleico conjugado (CLA) em produtos de origem animal e seus benefícios à saúde humana. Nesse contexto, em alimentos de origem animal é possível encontrar esse composto natural, principalmente na gordura do leite, carne e em alimentos naturalmente enriquecidos com este ácido e seus isômeros. O CLA tem propriedades bioativas distintas e é originário da biohidrogenação incompleta dos ácidos linoleico e linolênico realizada pelas bactérias ruminais ou da desaturação do trans-11 na glândula mamária e no intestino delgado. Os benefícios biológicos do CLA e dos isômeros cis-9 e trans-11, como um componente natural da gordura do leite, tem efeito anticarcinogênico, incluindo carcinomas na epiderme, tecido mamário e gastrintestinal. Dietas suplementadas com gorduras e a relação concentrado: volumoso, assim como a efetividade da fibra do volumoso podem aumentar a produção de leite, diminuir a produção de gordura e alterar seu perfil em ácidos graxos. Pesquisas avaliando os níveis de CLA nos produtos de origem animal e o seu efeito benéfico sobre a saúde e qualidade de vida do homem têm apresentado resultados satisfatórios, embora muitas vezes controversos, sendo necessários novos estudos para comprovar os resultados e a segurança na utilização desse composto, para que possa se determinar a sua real eficácia.

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CLA Reduces Inflammatory Mediators from A427 Human Lung Cancer Cells and A427 Conditioned Medium Promotes Differentiation of C2C12 Murine Muscle Cells

Cited by 17 publications

References 49 publications

Effects of conditioned media from murine lung cancer cells and human tumor cells on cultured myotubes

Effects of conditioned media from murine lung cancer cells and human tumor cells on cultured myotubes

Activated Lymphocytes Secretome Inhibits Differentiation and Induces Proliferation of C2C12 Myoblasts

Importância Do Ácido Linoleico Conjugado (Cla) Em Produtos De Origem Animal

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