Clarias batrachus collagen extract increases fibroblast cell adhesion, migration and proliferation

Abstract

Collagen is an extracellular matrix protein with great importance in biomedical application. The search for collagen from various sources is intensified especially from marine source. This study was carried out to extract collagen from a Malaysian local fresh water fish, Clarias batrachus and characterized its biomedical potential In vitro. Collagen was extracted from C. batrachus skin using acetic acid method and identified using SDS-PAGE. MTT assay was performed to determine the effect of coated collagen on cell adhesion and proliferation of L929 skin fibroblast cells. Additionally, scratch assay was performed to examine the effect of collagen coating on fibroblast cell migration. Result showed that collagen extracted from C. batrachus was made up of collagen type I, which consists of two α chains (α-1 and α-2) and β chain. At 100 µg/cm 2 density, collagen coating significantly increased fibroblast cell adhesion, proliferation and migration compared to negative control (p< 0.05). As a conclusion, collagen extracted from C. batrachus increased cell adhesion, proliferation and migration of fibroblast cells and has potential to be used as an alternative source of collagen.

Abalone Collagen Extracts Potentiate Stem Cell Properties of Human Epidermal Keratinocytes

“…There are some disadvantages of these sources; for instance, forbidden usage due to religious restriction, and contamination of several transmitted diseases such as bovine spongiform encephalopathy (BSE) and transmissible spongiform encephalopathy (TSE), leading to a decrease in these use of collagen sources [26,33]. Therefore, marine collagen sources from fishes [34,35,36], sponges [37], squids [38], jellyfish [39], and others gain high interest due to no risk of transmitted diseases, their higher collagen content, low antigenicity, and a reduction in environmental waste [33].…”

“…It has been used in traditional medicine and found to have multiple biological activities, such as anti-oxidant, anti-inflammatory, and anti-cancer activities [40,41]. Many studies have found that marine collagen extracts could increase cell proliferation and the migration of skin cells [35,36,42,43]. However, no research on the effects of abalone collagen on human keratinocytes has been conducted.…”

Evaluation of the biocompatibility of fish skin collagen with the mesenchymal stem cells in in vitro cultures

“…37,38 Furthermore, some literature sources reported the suitability of African sharptooth catfish collagen in the acceleration of wound healing, and as a scaffold for tissue engineering. 39,40 The results of our research imply that collagen extracted from African sharptooth catfish and silver carp skins is biocompatible, in terms of ASCs growth, clonogenic potential, and differentiation in osteogenic and adipogenic lineages. Furthermore, fish collagen due to its anti-inflammatory and immune-modulatory properties, ability to stimulate cell adhesion, can improve wound resolution and closure, and has potential in bone and cartilage tissue engineering.…”

Advances in surface modifications of the silicone breast implant and impact on its biocompatibility and biointegration

“…Although close to 28 proteins have been identified to belong to the collagen family, most of the collagens in the body are types I, II and III collagens [279]. Type I collagen is the most used collagen for coating silicone implants partly due to it being the dominant collagen within connective tissue and has been linked to enhanced fibroblast proliferation [281][282][283]. Being non-toxic as well as being biodegradable makes type I collagen a suitable material for coating silicone implant surfaces.…”