Class I aldolases: Substrate specificity, mechanism, inhibitors and structural aspects

Crystal structure of the reduced Schiff‐base intermediate complex of transaldolase B from escherichia coli: Mechanistic implications for class I aldolases

“…The mechanism proposed here for transaldolase shares several features with the enzymatic mechanisms that have been proposed for F-1,6-P aldolase (Littlechild & Watson, 1993;Gefflaut et al, 1995). Residues corresponding to Asp 17 and Glu 96 are also found at the active site of F-1,6-P aldolases, Asp 33, and Glu 197, respectively.…”

“…Site-directed mutagenesis of residue Asp 33 in F-1,6-P aldolase results in a large drop in catalytic activity, and it had been proposed that this residue might act as proton acceptor from the C4 hydroxyl of the substrate during catalysis (Morris & Tolan, 1993). Mechanistic proposals for the catalytic role of Glu 187 in F-1,6-P aldolase include the maintenance of a neutral electrostatic potential at the active site (Littlechild & Watson, 1993) or participation in the dehydration step of the carbinolamine (Gefflaut et al, 1995).…”

Highly Selective Inhibitors of Class II Microbial Fructose Bis-phosphate Aldolases

“…Surprisingly, in the same time, the preparation and evaluation of dozens of nonselective inhibitors of FBA have been reported. 10 We reasoned that an "ideal" selective inhibitor should have the following characteristics:…”

The crystal structure and identification of NQM1/YGR043C, a transaldolase from Saccharomyces cerevisiae

“…1 Different from the members of the class II adolase family, transaldolase is characterized by the formation of a covalent Schiff base intermediate between a lysine residue within the active site and the substrate. [2][3][4] As a key enzyme in the pentose phosphate pathway, transaldolase catalyzes the reversible transfer of a dihydroxyacetone moiety from fructose 6-phosphate to erythrose 4-phosphate and forms sedoheptulose 7-phosphate and glyceraldehyde 3-phosphate, respectively. 4-6 The vital functions of this enzyme are reported to balance the levels of NADPH and reactive oxygen intermediates (ROI) in the pentose phosphate pathway of glucose metabolism 7 and also to maintain the mitochondrial transmembrane potential and sperm fertility.…”