Classes and subclasses of rat immunoglobulins: Interaction with the complement system and with staphylococcal protein A

Medgyesi, G; ̈st, G. Fu; Gergely, J.; Bazin, Hervé

doi:10.1016/0161-5890(78)90052-4

Cited by 122 publications

(24 citation statements)

References 27 publications

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“…Monoclonal antibody secreted by 9B 18 and 13ED 2a4 was found to belong to the IgG2a subclass, which does not bind staphylococcal Protein A (SPA) (Medgyesi et al, 1978). The IgG from 13NA 2c was of the IgG1 subclass, and thus did bind SpA.…”

Section: Resultsmentioning

confidence: 96%

“…Immunoglobulin was precipitated by addition of saturated (NH4)2SO ~ solution to a final concentration of 50 ~. In view of the lack of Protein A sites on the most frequent subclasses of rat I gG (Medgyesi et al, 1978), purification based on their presence was not routinely attempted. The immunoglobulin concentration was adjusted to about 1 mg/ml by ultrafiltration (Cx30, Millipore).…”

Section: Cells and Virusesmentioning

confidence: 99%

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Monoclonal Antibodies to Murine Retrovirus Protein p30

Chuat

Grce

Pilon

et al. 1985

Journal of General Virology

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SUMMARYHybrid cell lines were prepared by the fusion of mouse myeloma cells with the spleen cells of Wistar-Furth rats that had been immunized with a Moloney sarcoma virus (MoMuSV)-induced tumour, MFU. Two immunization protocols were designed. In the first, the animals received several injections of irradiated (10 000 rad) cells of a tumour cell line established in vitro, MFU-67. The rats received a booster injection 3 days prior to fusion. In the second protocol, immunization was the result of simple tumour growth, and no booster was given. Hybrids were tested by immunofluorescence for the production of immunoglobulins reacting with mouse cells acutely infected with MoMuSV. Over 20 ~ of reactive hybrids were observed in the tumour growth protocol, and about 10 ~o in the irradiated cell protocol when the last injection of the series was given 2 weeks before fusion. After 6 months, the proportion fell to 3~. Hybrid lines producing antibody to p30, the major core polypeptide of murine retroviruses, were obtained by cloning. Three of these were selected for closer study and were found to recognize three non-overlapping epitopes on p30. By direct and competitive binding in ELISA tests, the three epitopes were found to have very different distribution patterns among the various strains and isolates of murine retroviruses.

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Section: Resultsmentioning

confidence: 96%

Section: Cells and Virusesmentioning

confidence: 99%

Monoclonal Antibodies to Murine Retrovirus Protein p30

Chuat

Grce

Pilon

et al. 1985

Journal of General Virology

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“…24,25 Complement by IgG antibodies proceeds through the classic pathway, as demonstrated by C1 binding. 26 In other words, IgG forms the classic pathway convertase C4bC2a through C1 fixation and subsequently cleaves C3 into C3a and C3b. A greater number of complement-activating moieties in the mesangial area result in more C3 convertase formation, including the alternative pathway convertase C3bBb.…”

Section: Discussionmentioning

confidence: 99%

Glomerular IgG deposition predicts renal outcome in patients with IgA nephropathy

et al. 2016

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Glomerular IgG deposition is frequently observed in patients with IgA nephropathy. However, the association between glomerular IgG deposition and progression of IgA nephropathy is uncertain. Six hundred and twentyseven patients with biopsy-proven IgA nephropathy were recruited. Histological variables of the Oxford classification (Oxford-MEST) and the presence of glomerular IgG deposits were assessed. Renal progression defined as end-stage renal disease or 50% reduction in estimated glomerular filtration rate was analyzed using Kaplan-Meier methods and Cox regression analysis. Of the study population, 200 patients (31.9%) had glomerular IgG deposition on immunofluorescence staining. During a mean follow-up of 56.8 ± 37.5 months, the rate of renal progression was significantly higher in the IgA nephropathy patients with glomerular IgG deposition compared with the IgA nephropathy patients without glomerular IgG deposition (39.8 vs 12.3 per 1000 patientyears; Po 0.001). Of patients with IgG deposition, 178 (28.3%), 20 (3.2%), and 2 (0.3%) patients had mild, moderate, and marked glomerular IgG deposits, receptively. Kaplan-Meier analysis revealed that cumulative renal survival was significantly lower in IgA nephropathy patients with the higher intensity of glomerular IgG deposits (Po 0.001). In addition, Cox regression analysis revealed that moderate and marked glomerular IgG deposits significantly predicted renal outcome independent of Oxford-MEST and clinical variables (HR, 2.97; 95% CI, 1.01-8.77; P = 0.04). This study showed that that glomerular IgG deposition was independently associated with poor renal outcome in patient with IgA nephropathy.

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“…The ligand of choice was rat IgE. Our source of rat IgE, the immunoglobulin fraction secreted from IR162 tumors, has been well characterized (Bazin et al, 1974;Medgyesi et al, 1978). The structure of the bi-antennary complex oligosaccharide carried by rat IgE, which binds with high affinity to PSL, has been published (Kornfeld et al, 1981) and corresponds to glycopeptide 1 obtained from human lactotransferrin (Debray et al, 1981).…”

Section: Rat Ige Has a Glycan Moiety That Binds Pslmentioning

confidence: 99%

Sugar-Binding Activity of Pea Lectin Expressed in White Clover Hairy Roots

et al. 1995

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Dénarié et al., 1992). Nodulation is regulated by a two-way exchange of plant and bacterial signal molecules (Fisher and Long, 1992;Spaink et al., 1993). Signal exchange begins in the rhizosphere. Rhizobia sense flavonoids secreted by seeds and by the host plant root, which activate the constitutively expressed nodD gene product (reviewed by Schlaman et al., 1992). This step is a determinant of host plant specificity in some Rhizobium-host plant combinations (Horvath et al., 1987; Spaink et al., 1987). Transcription of structural nod genes is under the control of NodD and results in production and secretion of specific lipochitin oligosaccharides (Nod factors), which act as host plant-specific inducers of nodule morphogenesis (reviewed by Dénarié and Cullimore, 1993;Spaink et al., 1993). Most probably, Nod factors constitute the primary determinants of host-plant specificity of nodulation.Root lectins are plant factors involved in nodulation. Lectins are nonenzymatic sugar-binding (g1yco)proteins characterized by sugar-binding specificity. For example, root PSL is Glc/Man specific (Díaz et al., 1990) and white clover root lectin is 2-deoxyglucose specific (Sherwood et al., 1984). In general, the interaction between RI uiciae and white clover does not proceed beyond root hair curling, and these bacteria are not able to penetrate host plant cells by infection thread formation (Yao and Vincent, 1969;Djordjevic et al., 1986;Huang et al., 1993). However, RI viciae is able to induce infection thread formation and nodulation in a significant percentage of white clover plants with roots transformed with the psl gene, as reported by Díaz et al. (1989Díaz et al. ( , 1995. This heterologous nodulation does not take place when a psl mutant, which encodes a non-sugar-binding protein, is used for transformation of white clover roots (Van Eijsden et al., 1992. These results strongly suggest that (a) PSL is functionally expressed in transgenic white clover roots, (b) conservation of the sugar-binding activity of PSL is necessary for infection of white clover by RI viciae, and (c) root lectin helps to break a host plant-specific barrier for nodulation. The relationship between Nod factor recognition by host plants and Rhizobium recognition by host lectin is not yet clear.PSL is an ellipsoidal, nonglycosylated dimeric lectin with two sugar-binding sites, each located near the top of two identical monomers (Einspahr et al., 1988). PSL is encoded by a single gene (Gatehouse et al., 1987;Kaminski et al., 1987) and is synthesized as a single translation product, a prolectin with a molecular mass of 28 kD. Posttranslational modifications result in a protein that dissociates into two p subunits (18 kD each) and two 01 subunits (6 kD each, Higgins et al., 1983)

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Classes and subclasses of rat immunoglobulins: Interaction with the complement system and with staphylococcal protein A

Cited by 122 publications

References 27 publications

Monoclonal Antibodies to Murine Retrovirus Protein p30

Monoclonal Antibodies to Murine Retrovirus Protein p30

Glomerular IgG deposition predicts renal outcome in patients with IgA nephropathy

Sugar-Binding Activity of Pea Lectin Expressed in White Clover Hairy Roots

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