Classical and molecular cytogenetic analysis in head and neck squamous cell carcinomas

Abstract

Head and neck carcinomas represent the sixth most frequent type of cancer in the world, and 90% are derived from squamous cells (HNSCC). In this study of 15 HNSCC cases, extensive aneuploidy was detected by G banding in most tumors. The most frequently observed numerical changes involved gain of a chromosome 22, and loss of chromosomes Y, 10, 17, and 19. The most frequent structural alteration was del(22)(q13.1). As compared to G-banding, fluorescence in situ hybridization (FISH) proved to be an effective technique for detecting aneuploidy. Interphase FISH with a chromosome 17 centromere probe disclosed a high frequency of monosomy for chromosome 17, in contrast with G-banding, by which clonal monosomy 17 was detected in only three of the tumors. Painting probes for chromosomes 5 and 16 were used to evaluate a selected series of HNSCC in which G-banding analysis had shown marker chromosomes. FISH analysis failed to confirm the origin of the marker chromosomes, but four out of five cases showed a significant loss of chromosomes 5. This difference between FISH and G-banding results may reflect the smaller number of metaphase analyzed as well as the criteria adopted for sorting these metaphases. Therefore results obtained solely by G-banding analysis should be considered with caution. Our data confirmed the involvement of chromosome 17 in head and neck squamous cell carcinomas.

Alterations in p16 and p53 genes and chromosomal findings in patients with lung cancer: Fluorescence in situ hybridization and cytogenetic studies

“…However, we have observed a high frequency of monosomy for chromosome 17 in LC patients. Veiga et al also show a high frequency of monosomy 17 by interphase FISH in only three of the tumors [28]. Our cytogenetic results, in addition to other previously reported findings, suggest that losses of chromosome 17 play a role in the pathogenesis of LC.…”

“…These chromosomes may affect the susceptibility to tumors. In other studies, the most frequently observed numerical changes involve the gain (chromosomes 7, 20, and 22) and loss (1, Y, 10, 17, and 19) of chromosomes, and the most common structural rearrangements are deletions of chromosomes 3, 17, and 9 [28,29].…”

Detección de aneuploidías del cromosoma 17 y deleción del gen TP53 en una amplia variedad de tumores sólidos mediante hibridación in situ fluorescente bicolor

“…Este porcentaje es muy alto comparado con los publicados por otros autores en diferentes tumores sólidos (25,(35)(36)(37)(38); sin embargo, está dentro del rango informado y concuerda con lo descrito en la literatura en lo referente a que este gen se encuentra alterado en cerca de 50% de todos los canceres en humanos (7,9,10,16,22).…”

Deciphering the Role of the Barr Body in Malignancy: An insight into head and neck cancer

“…38 Martin et al previously published a thorough description of specific genetic changes involving autosomes in oral squamous cell carcinoma cases, which included the loss of chromosomal segments 3p, 5q, 7q, 8p, 9p, 11q and 18q in addition to the gain of 3q, 5p, 7p, 8q and 11q …”